Prooxidant-antioxidant homeostasis in the incubed sperm of boars at feeding lactates of microelements

Усенко, С. О.; Shostya, A. M.; Stoianovskyi, V. G.; Birta, Gabriella; Kuzmenko, L. M.; Slynko, V. G.

doi:10.31548/dopovidi2020.02.017

Cited by 2 publications

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“…Однак зі збільшенням кількості згодовуваної міді (20%) відбувається прискорення процесів пероксидації у спермі кнурівплідників, що проявляється у підвищенні вмісту метаболітів -дієнових кон'югантів і ТБК-активних сполук та водночас інтенсивним використанням глутатіону та активацією СОД. Про близькі особливості формування ПАГ у спермі цього виду тварин після вживання великих доз мікроелемента повідомляли С. О. Усенко та В. O. Рокотянська [14,19]. Це пов'язано з тим, що мідь у формі вільного металу є каталізатором при утворенні супероксидних та гідроксильних радикалів (реакція Габера-Вейса) [15].…”

Section: обговоренняunclassified

Prooxidant-antioxidant homeostasis and reproductive capacity of boars under the influence of copper citrate

Siabro¹,

str.²

2021

Bìol. Tvarin

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Peroxide oxidation processes play a leading role in ensuring the motility, survival and fertilizing ability of sperm. A special role is given to limiting antioxidants (vitamins, amino acids, microelements). Therefore, the development of standardized feeding programs to provide antioxidant nutrition is one of the effective methods of reproductive biotechnology. The aim of the study was to determine the effect of copper citrate on the quality of sperm production and the formation of prooxidant-antioxidant homeostasis in sperm of boars. The experiment used adult boars of a large white breed, analogs in age, live weight and quality of sperm products. Experimental groups were fed copper citrate above the norm by 10% and 20%. It has been determined that feeding combined feed to boars with the addition of this compound in an amount of 10% above norm probably increases the weight of ejaculate by 12.5% (P<0.05), the sperm motility and survival by 6.5% (P<0.01) and 13.5% (P<0.001), respectively. Such changes in sperm occur against the background of an increase in SOD activity by 80.6% (P<0.05), a decrease in catalase by 43.5% (P<0.05), a slowing down of peroxidation processes — a decrease in diene conjugates and TBA-active compounds. The additional introduction to the diet of copper citrate by 20% more than normal increases the concentration of spermatozoa by 13.2% (P<0.01), the number of live spermatozoa by 20.7% (P<0,01), with a simultaneous decrease in their survival, due to the acceleration of peroxidation processes — an increase in the content of diene conjugates, TBA-active compounds and DAA and a decrease in reduced glutathione. It has been found out that the fertilizing ability of sperm significantly depended on the amount of fed microelement. Sows inseminated with sperm of boars receiving copper supplement in the diet by 10%, had higher fertility rates by 7.1%, multifertility by 3.6%, and a litter weight at weaning by 8.8%. The additional administration of copper citrate reduced the fertility of sperm by 20%, as the fertility rate of sows of III group was the lowest and was 7.7% and 14.3% lower compared to I and II groups. A similar trend occurred in terms of high fertility, a litter weight at birth and weaning. Therefore, the additional feeding of a small amount of copper has a positive effect on the functional activity of sperm and the processes of normal fertilization, growth and development of embryos and newborn piglets by optimizing the formation of prooxidant-antioxidant homeostasis.

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Section: обговоренняunclassified

Prooxidant-antioxidant homeostasis and reproductive capacity of boars under the influence of copper citrate

Siabro¹,

str.²

2021

Bìol. Tvarin

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“…(Danchuk, 2018) доведено вплив окремих типів вищої нервової діяльності (ВНД) на формування прооксидантноантиоксидантого гомеостазу у свиней різного віку за дії різних стресових чинників. Зміна даних гомеоста-тичних констант істотно впливає на якість спермопродукції, функціональну активність сперміїв та здатність їх до запліднення (Usenko et al, 2020). Коригування даних процесів шляхом додавання в корм сполук антиоксидантної дії дозволяє оптимізувати їх відтворювальну функцію.…”

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Prooxidant-antioxidant homeostasis in boars depending on the types of higher nervous activity

et al. 2020

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The peculiarities of formation of prooxidant-antioxidant homeostasis (PAG) in boars of different types of higher nervous activity have been studied. Using motor-nutritional techniques, it has been opposed adult boars of the Large White breed aged 24–36 months according to the main types of higher nervous activity (HNA). Four groups of 3 heads each were formed. The first group includes animals of a strong balanced lively (mobile) type; group II includes a strong balanced calm (inert) type; group III includes a strong unbalanced (unrestrained) type; group IV includes a weak type of higher nervous activity. Sperm was received from boars manually, taking into account the total time of ejaculation, dividing the ejaculate into 4 fractions – F1 – the first, F2 – the second, F3 – the third, F4 – the fourth. PAGs in blood and ejaculates of boars were evaluated by the intensity of peroxidation processes (xanthine oxidase, diene conjugates, TBA-active compounds) and antioxidant defense systems (superoxide dismutase, catalase, reduced glutathione, ascorbic acid, vitamin A and vitamin E). Sperm contained higher amounts of diene conjugates and TBA-active complexes (P < 0.05) compared to blood. The second tissue relative to the first one is characterized by a higher level of antioxidant defense: catalase activity, concentration of ascorbic acid, vitamin A and vitamin E. The process of PAG formation in the tissues of boars depends on the type of higher nervous activity (HNA). Thus, in individuals of a strong balanced lively and calm type, the course of peroxidation processes is somewhat slower because of a lower concentration of diene conjugates and TBA-active complexes. It was determined the fact that in blood and sperm of boars of strong unbalanced and weak types of HNA the course of peroxidation is more intense, the antioxidant defense system is at a lower level that is connected with less superoxide dismutase activity (P < 0.05), concentration of ascorbic acid (P < 0.001), vitamin A (P < 0.01–0.001), and vitamin E (P < 0.001). In the second and third fractions of ejaculate of boars, the PAG is shifted in the direction of probable acceleration of peroxidation processes, especially in strong unbalanced and weak types of HNA because of a higher concentration of diene conjugates, dehydroascorbic acid. Animals of a strong balanced lively and calm types of HNA are characterized by a higher level of antioxidant defence – catalase activity, superoxide dismutase, reduced glutathione, ascorbic acid and vitamin E. In the first and fourth fractions of ejaculate, peroxidation processes are slower and there is no superoxide dismutase activity and the amount of vitamin A and vitamin E.

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Prooxidant-antioxidant homeostasis in the incubed sperm of boars at feeding lactates of microelements

Cited by 2 publications

References 5 publications

Prooxidant-antioxidant homeostasis and reproductive capacity of boars under the influence of copper citrate

Prooxidant-antioxidant homeostasis and reproductive capacity of boars under the influence of copper citrate

Prooxidant-antioxidant homeostasis in boars depending on the types of higher nervous activity

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