Propagación in vitro de Annona deceptrix (westra) H. Rainer (Annonaceae) una especie en peligro de extinción en Ecuador

Abstract

This work aims to develop a protocol for the in vitro propagation of Annona deceptrix (westra) H. Rainer. In addition, it measures survival in ex-vitro conditions. Nodal shoot segments were used as explants, disinfected with different NaClO concentrations and exposure times, and then precultured in the Murashige and Skoog (MS) basal culture medium. Woody Plant Medium (WPM) was used for the multiplication and rooting phase, with different types and concentrations of phytoregulators. In the establishment phase, the explants responded better with the 1% NaClO treatment for 15 minutes, where the highest survival percentage was observed at 72.04%. For the variable number of shoots and stem diameter, the treatment with BAP (1 mgL-1) + (GA3 0.25 gL-1) + (WPM) reached the best average with a value of 2.00 and 2.35, respectively. Mm, respectively. For root induction, the best response was obtained when 3mgL-1 IBA (T3) was added to the culture medium, reaching an average root length of 5.75 cm after 60 days of culture in the medium. The rooted Vitro plants of an approximate height of 5 cm were brought to the mesh house for their acclimatization; in this phase, the survival rate was evaluated during the first week of acclimatization, and the plant's growth fifteen days after sowing in trays containing a peat-based substrate, where T3 had an average survival of 70% and higher plant height at 60 days, indicating that this treatment promoted faster seedling development under conditions exvitro. Keywords: Annona deceptrix, propagation, in vitro rooting, ex-vitro acclimatization, growth regulators.