Propagation in Tissue Cultures of Cytopathogenic Agents from Patients with Measles.

Enders, John F.; Peebles, T. C.

doi:10.3181/00379727-86-21073

Cited by 586 publications

(203 citation statements)

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“…In 1941 (i) an examination of cases from an epidemic of primary infant virus pneumonitis showed the existence of cytoplasmic inclusion bodies within epithelial ceils of the lungs. Later it was shown that morphologically similar inclusion bodies (both nuclear and cytoplasmic) can be found in distemper infections of clogs and ferrets (2,5,14), and in tissue culture cells infected with measles virus (5,8). A few instances of inclusion material appearing like that found in measles and distemper infections have come from a variety of pathological sources (14,4).…”

Section: Introductionmentioning

confidence: 99%

Fine Structure of Cellular Inclusions in Measles Virus Infections

Kallman

Adams

Williams

et al. 1959

The Journal of Cell Biology

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Cells which are infected with measles virus have been known for some time to contain inclusion material that is distinguishable from normal cellular components by application of traditional staining methods and observation in the light microscope. The fine structure of the inclusion material contained in HeLa cells infected with Edmonston strain of measles virus has been examined in the electron microscope. Two steps have been found necessary in this study: (1) the recognition by phase-contrast microscopy of the living cell of bodies that are defined as inclusion material when the cells are classically stained; and (2) the recognition in the electron microscope of inclusion-body material that had previously been identified in the living cell.The fine structure of the nuclear and cytoplasmic inclusion material in osmiumtreated cells was found to consist mainly of randomly arrayed filaments of low electron density. Dense, highly ordered arrays of filaments were found near the center of the nuclear inclusions, sometimes as a two-dimensional, nearly orthogonal arrangement. If the size of the measles virus is taken to be around 100 m/z in diameter, the strands seen in the inclusions cannot be fully formed virus.

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Section: Introductionmentioning

confidence: 99%

Fine Structure of Cellular Inclusions in Measles Virus Infections

Kallman

Adams

Williams

et al. 1959

The Journal of Cell Biology

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“…MV was first isolated by Enders & Peebles (1954) using primary human kidney cell cultures. One of the isolates (Edmonston strain) has been passaged afterwards in many laboratories using monkey kidney cell lines such as Vero and CV-1 cells, and used for analysis as a standard strain of MV.…”

Section: Introductionmentioning

confidence: 99%

Increased binding activity of measles virus to monkey red blood cells after long-term passage in Vero cell cultures

Shibahara

Hotta

Katayama

et al. 1994

Journal of General Virology

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Recent field isolates of measles virus (MV) obtained by using B95-8 cells have been reported not to agglutinate African green monkey red blood cells (AGM-RBC). Vero cell-adapted, plaque-forming strains derived from three field isolates at the third passage in Vero cell cultures (T8Ve-3, T11Ve-3 and N13Ve-3) also exhibited markedly decreased binding activity, as determined by infectivity-absorption and haemadsorption tests. On the other hand, binding activity of the respective strains at the twentieth passage

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“…Starting with the demonstration that MV could be propagated in human and simian cell cultures (Enders and Peebles, 1954), a considerable amount of data has been published on MV production. MV amplification has been described in various cell lines and with various culture modes.…”

Section: Measles Virus Productionmentioning

confidence: 99%

“…The isolated strain has been derived from the throat washings of an 11 years old measles patient named David Edmonston and was therefore named Edmonston strain (Enders and Peebles, 1954). This measles virus strain was adapted to cell culture and Science Publications AJBB attenuated by serial tissue culture passages resulting in less virulent virus strains, e.g., Edmonston B.…”

Section: Application Of Attenuated Measles Virusmentioning

confidence: 99%

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Key Parameters of Measles Virus Production for Oncolytic Virotherapy

Weiss

Salzig

Mühlebach³

et al. 2012

American Journal of Biochemistry and Biotechnology

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Attenuated measles virus has revealed selective tumor cell killing and is currently tested in clinical trials for the therapy of cancer patients. The amount of infectious particles per dose needed for oncolytic therapy can be more than a million times higher compared to vaccination. This requires highly effective production processes which guarantee the measles virus quantities needed for its use in cancer therapy. Referring to measles virus production itself, several factors are influencing process parameters and subsequent virus yields. These factors are medium optimization, feeding of nutrients, an optimal multiplicity of infection, localization of produced virus particles, temperature sensitivity and time of harvest. This review summarizes the available data concerning measles virus production in cell culture and factors influencing the virus yield.

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Propagation in Tissue Cultures of Cytopathogenic Agents from Patients with Measles.

Cited by 586 publications

References 0 publications

Fine Structure of Cellular Inclusions in Measles Virus Infections

Fine Structure of Cellular Inclusions in Measles Virus Infections

Increased binding activity of measles virus to monkey red blood cells after long-term passage in Vero cell cultures

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

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