“…Historically, apices, internodes, nodes, young leaves, and axillary buds were used as explants for regeneration of B. pendula and B. pubescens (Huhtinen, Yahyaoglu, 1974;Chalupa, 1981;Srivastava, Steinhauer, 198l;Simola, 1985;Ryynanen, Ryynanen, 1986). Currently, in vitro studies of birch continue to be carried out and focus largely on improvement of micropropagation conditions (Kontsevaya, 2009) and on investigation as to how environmental factors may influence rooting (Bojarczuk, 2000;Wynne et al, 2002) and general plant morphology (Ryynänen et al, 2005).…”