Propagation of elite rescue dogs by somatic cell nuclear transfer

Oh, Hyun Ju; Choi, Jin Nam; Kim, Min Jung; Jo, Yeonhwa; Choi, Yong-Sung; Lee, Byeong Chun

doi:10.1111/asj.12402

Cited by 11 publications

(12 citation statements)

References 31 publications

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“…Artificial activation of the reconstructed embryos was induced, and, immediately after activation, the embryos were transferred into oviducts of recipient dogs. Consequently, the resulting cloned puppy had the same nuclear and mitochondrial genetic information as the cell donor and oocyte donor, respectively [ 8 29 37 42 ]. Since that first cloned dog, a variety of cloned dogs have been produced, and it has been reported that dog cloning using the SCNT technique can be successfully performed regardless of the sex (male [ 36 ] and female [ 25 ]), age (fetus [ 18 ], young adult [ 36 ], and old [ 24 ]), or breed (Afghan hound [ 25 36 ], miniature poodle [ 24 ], beagle [ 18 29 ], Labrador Retriever [ 35 43 ], Pekingese [ 46 ], German Shepherd [ 30 42 ], Sapsaree [ 23 ], and Gyeongju Donggyeong dog [ 8 ]) of the cell donor.…”

Section: Introductionmentioning

confidence: 99%

Health and temperaments of cloned working dogs

Kim

Hwang³

et al. 2018

J Vet Sci

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Dogs serve human society in various ways by working at tasks that are based on their superior olfactory sensitivity. However, it has been reported that only about half of all trained dogs may qualify as working dogs through conventional breeding management because proper temperament and health are needed in addition to their innate scent detection ability. To overcome this low efficiency of breeding qualified working dogs, and to reduce the enormous costs of maintaining unqualified dogs, somatic cell nuclear transfer has been applied in the propagation of working dogs. Herein, we review the history of cloning working dogs and evaluate the health development, temperaments, and behavioral similarities among the cloned dogs. We also discuss concerns about dog cloning including those related to birth defects, lifespan, and cloning efficiency.

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Section: Introductionmentioning

confidence: 99%

Health and temperaments of cloned working dogs

Kim

Hwang³

et al. 2018

J Vet Sci

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“…The canine SCNT technique has been notably improved since Snuppy, the first cloned dog, was born in 2005 (Lee et al., ). This achievement made canine SCNT applicable to various fields such as conservation of endangered species (Choi et al, ; Jang et al, ; Kim et al, ; Oh et al., ), elite working dogs (Choi et al., ; Oh et al., , ) and animal model researches (Hong et al., ; Kim et al., , ).…”

Section: Application Of Canine Scntmentioning

confidence: 99%

“…Cloning dogs by SCNT is a highly effective way of producing dogs with exceptional skills, as they are identical to those of cell donor dogs except the mitochondrial genome. For instance, the elite cloned dogs with high ability for drug detection in airport/quarantine areas or rescuing human in disaster fields have been successfully generated (Kim et al, , ; Oh et al., , ). Furthermore, their ability to sniffing drugs was evaluated (Choi et al., ), and all of the cloned dogs with identical genomic backgrounds were selected as a drug detection dogs.…”

Section: Application Of Canine Scntmentioning

confidence: 99%

“…The Y‐maze test (Sanders et al, ), with some modifications, was selected in order to evaluate similarities in learning and memory between cloned and noncloned dogs. Another behavioural research was conducted in cloned dogs to evaluate the aptitudes of puppies as service dogs (Oh et al., ). Through the 10 subtests for puppy aptitude test, they exhibited a similar tendency for the test with the same behaviour type, although these cloned dogs did not show a perfectly identical score for test.…”

Section: Introductionmentioning

confidence: 99%

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Dog cloning—no longer science fiction

Lee

Kim

et al. 2018

Reprod Domestic Animals

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Contents Since the generation of world's first cloned dog, Snuppy, in 2005, somatic cell nuclear transfer (SCNT) in dogs has been widely applied for producing several kinds of dogs with specific objectives. Previous studies have demonstrated that cloned dogs show normal characteristics in growth, blood parameters and behavioural aspect. Also, canine SCNT technique has been applied to propagate working dogs with excellent abilities in fields such as assistance of disabled people, drugs detection and rescue activity. Because dogs have similar habituation properties and share many characteristics including anatomic and physiological aspects with humans, they are also primary candidates for human disease models. Recently, transgenic dogs that express red fluorescent protein gene constitutively and green fluorescent protein gene conditionally have been generated. In addition, transgenic dogs with an overexpression of peroxisome proliferator‐activated receptor‐alpha in specific muscles were generated to enhance physical performance. In 2017, Snuppy was recloned with markedly increased pregnancy and delivery rates compared to the statistics from when Snuppy was first cloned. Such striking improvements in the cloning of dogs using SCNT procedures suggest that dog cloning could be applied in many fields of biomedical science for human diseases research, and the application of cloning is no longer science fiction.

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“…Studies on canine embryos are scarce because of difficulties in collecting in vivo‐produced embryos and the inability to produce canine embryos in vitro (Chastant‐Maillard et al., ). So far, no puppy has been obtained from an in vitro‐produced embryo; however, life births after cloning of somatic cells have been repeatedly reported in domestic dogs (Hossein et al., ; Lee et al., ; Oh et al., ) as well as in coyotes and wolves (Hwang et al., ; Kim et al., ).…”

Section: Art In Dogs Cats and Other Carnivores – State Of The Artmentioning

confidence: 99%

Research on reproduction is essential for captive breeding of endangered carnivore species

Jewgenow

Braun

Dehnhard

et al. 2016

Reprod Domestic Animals

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Assisted reproductive technology (ART) has great potential for conservation, but its successful application in captive breeding programmes of endangered species is often compromised by limited background on species' biology. Although carnivore species benefit from knowledge obtained in domesticated species (dogs, cats and ferrets), the focus of research is different. In pet animals, research in reproduction has mainly been focused on ovarian function and contraception, although substantial progress has also been made in the field of in vitro embryo production, transgenic embryos and cloning to aid relevant medical models. In endangered species, however, research should focus on characterizing reproductive traits (cyclicity and seasonality) to unravel species-specific endocrine principles of reproduction physiology. Based on this knowledge, it is crucial to enhance the ability to manipulate female reproductive cycles, especially those of embryo recipients. Furthermore, research conducted on molecular and cellular mechanisms of gamete and embryo development, as well as on cryopreservation protocols of gametes and embryos, is required for successful implementation of advanced ART to wild carnivores. This review will provide a summary on the state of the art with focus on ART contributing to conservation breeding of endangered carnivores.

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Propagation of elite rescue dogs by somatic cell nuclear transfer

Cited by 11 publications

References 31 publications

Health and temperaments of cloned working dogs

Health and temperaments of cloned working dogs

Dog cloning—no longer science fiction

Research on reproduction is essential for captive breeding of endangered carnivore species

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