Propagation of fern (Nephrolepis) through tissue culture

Abstract

Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into entire plantlets. An overall multiplication factor of 30-40 was attained within three months.

In vitro proliferation and triterpenoid characteristics of licorice (Glycyrrhiza uralensis Fischer, Leguminosae) stolons

“…Shoot elongation, initiated in the dark, increased the number of explants (micronodes). Clonal propagation from stolon culture has been reported in fern (Padhya et al 1982) and potato (Dragicevic et al 2008). The present study is the first report of clonal propagation of licorice plants (G. uralensis) using stolon culture, and our stolon culture M. Kojoma et al…”

Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)

“…According to Stokes (1984), in vitro propagation of pteridophytes species can require prolonged periods of time, which sometimes might extend to several years, so that the frond and plant size can reach the uniformity demanded by the market of ornamental plants. During in vitro regeneration of ferns species, some factors affect the multiplication efficiency and many aspects have been examined, such as explant donor source (Atmane et al 2000;Whittier & Braggins 2000), nutrient type (Padhya & Mehta 1982;Fernández et al 1999) and physical culture conditions (Lin & Yang 1999). Sporophyte fragments have been used as explants source, which are usually the most practical and viable form of micropropagation of ornamental species in commercial scale .…”

In Vitro Conservation of Some Threatened and Economically Important Ferns Belonging to the Indian Subcontinent

“…The most prominent example of mass multiplication of an economically important fern is N. exaltata [18] in which callus induction was reported from provascular tissue of terminal and lateral buds of stolon tips. Some other reports are available on other species of Nephrolepis, N. cordifolia [19][20][21], and N. exaltata [14,[22][23][24][25], on morphogenetic studies pertaining to the micropropagation on a small scale. In vitro-regenerated plants had to be acclimatized in an inorganic salt solution for about two weeks in order to get 100 percent transplantation success in soil.…”