The production of gladiolus cut flowers of good quality largely relies on corm reserves and size; however, little attention has been paid to the understanding of the nutrient requirements for optimum quality of such organs. In the present study, it was evaluated the dynamics of biomass and the content of macronutrients in the mother and daughter corms as affected by the initial size of the mother corm. The biomass of the mother corm decreased after the transplant whereas, after flower harvest, that of the daughter corm increased. The content of N, K, Mg and S had a similar tendency as that of biomass, however, once the daughter corm appeared there was an increase in the content of these elements. A portion of P, Ca, Mg and S present in the mother corm was remobilized towards the daughter corm, nonetheless, in order to meet its demands, the daughter corm has to be supplemented through fertilization with 63.6, 52.9, y 38.0 kg ha-1 N, 9.2, 8.4 y 5.8 kg ha-1 P and 11.2, 11.3, and 7.7 kg ha-1 K for daughter corms developed from mother corms of 3.8 g (3.5 cm diameter), 2.5 g (3.0 cm diameter) and 1.8 g (2.5 cm diameter), respectively. Calcium has to be supplemented at 2.9-5.0 kg ha-1 , whereas Mg and S at 0.9 and 1.6 kg ha-1, respectively.