Propagation of Proteaceae

Malan, D.G.

doi:10.17660/actahortic.1992.316.5

Cited by 23 publications

(15 citation statements)

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“…In particular, it has a poor physiological capacity for adventitious root formation. Conventional propagation methods used to propagate P. cynaroides are slow and inconsistent and typically have low success rates (Malan, 1992). Advances in the in vitro establishment of P. cynaroides nodal explants (Wu and du Toit, 2004) and apical buds (Thillerot et al, 2006) have been made.…”

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Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Wu¹,

Lin²

2012

horts

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The effects of light quality emitted by light-emitting diodes (LEDs) on the growth and morphogenesis, and concentrations of endogenous phenolic compounds of Protea cynaroides L. plantlets in vitro, were investigated. Plantlets were cultured under four light treatments: conventional fluorescent lamps (control), red LEDs (630 nm), blue LEDs (460 nm), and red + blue LEDs (1:1 photosynthetic photon flux). Four phenolic compounds extracted from the plantlets were analyzed: 3,4-dihydroxybenzoic acid, gallic acid, caffeic acid, and ferulic acid. The highest rooting percentage was observed in plantlets cultured under red LEDs (67%) compared with 7% under conventional white fluorescent light, 13% under blue LEDs, and 13% under red + blue LEDs. The highest number of roots per plantlet was also found under red LEDs, whereas a significantly lower number of roots per plantlet was obtained under the other light treatments. Furthermore, red light promoted the formation of new leaves in P. cynaroides plantlets. However, the highest leaf dry weight (53.8 mg per plantlet) was found in plantlets irradiated by the combination of red and blue LEDs. Phenolic analyses showed that the lowest concentrations of 3,4-dihydroxybenzoic acid (4.3 mg·g−1), gallic acid (7.0 mg·g−1), and ferulic acid (7.4 mg·g−1) were detected in plantlets exposed to red light, whereas those irradiated by white fluorescent light contained the highest concentration. A significant inverse correlation (r = –0.419) was established between 3,4-dihydroxybenzoic acid and rooting percentage. Strong inverse correlations were also established between 3,4-dihydroxybenzoic acid and number of roots per plantlet (r = –0.768) as well as between ferulic acid and number of roots per plantlet (r = –0.732). These results indicate that the stimulation of root formation in P. cynaroides plantlets under red LEDs is the result of the low endogenous concentrations of 3,4-dihydroxybenzoic acid and ferulic acid.

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confidence: 99%

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Wu¹,

Lin²

2012

horts

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“…On average, terminal cuttings had a higher rooting percentage (53.8%) compared to subterminal cuttings (32.6%) and produced on average 1.94 roots per cutting with a longest root of 16.56 mm compared to 0.79 roots and 12.55 mm, respectively, for subterminal cuttings (Table 3). Terminal cuttings are generally believed to give the highest rooting percentage and are usually recommended for propagation of most cuttings, although some commercial nurseries also use subterminal cuttings with good results (Brits 1986, Harré 1988, Malan 1992. From this study, it was clear that terminal cuttings rooted better than subterminal cuttings and, thus, would be ideal to use for rooting purposes.…”

Section: Short Communicationmentioning

confidence: 75%

Effect of species, cutting position and exogenous rooting substances on rooting of honeybush (Cyclopiaspp.) cuttings

Mbangcolo

Reinten

Agenbag

2013

South African Journal of Plant and Soil

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Effect of the cutting position, terminal and subterminal, and rooting substance on the rooting of cuttings of two Cyclopia species (Cyclopia genistoides and C. intermedia) taken in winter, spring or summer was studied under controlled glasshouse conditions. Cuttings taken in summer, irrespective of the species, rooted better than cuttings taken either in winter or spring. This poor rooting performance of cuttings in winter and spring resulted in no significant treatment effects and for this reason only results of cuttings taken in summer are discussed. Cyclopia genistoides rooted significantly better than C. intermedia as measured by rooting percentage, number of roots per cutting, length of longest root and mean root length. Terminal cuttings from C. genistoides also increased rooting percentage and number of roots compared to subterminal cuttings, but not so in C. intermedia. Longest roots were produced by terminal cuttings irrespective of species. Treatment with different exogenous rooting substances as a main factor did not have any significant effect on rooting in this study.

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“…The need for a study on the action of antioxidant treatments on the in vitro culture of this plant arises from the Proteaceae being rich in phenolic compounds (Malan, 1992;Watad et al, 1992b;Rugge, 1995;Wu 794 E. Suárez et al / Span J Agric Res (2010) and du Toit, 2004). After the dissection of explants, these compounds may be oxidized by different enzymes (polyphenoloxidases and tyrosinases) which are synthesized and/or liberated due to injury during excision and sterilization of tissues (Torres, 1989).…”

Section: Discussionmentioning

confidence: 99%

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

Suárez

Pérez-Francés

Rodríguez-Pérez

2010

Span. j. agric. res.

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Leucadendron 'Safari Sunset' is one of the most valuable Proteaceae cultivar, due to its economic importance as cutflower. In vitro tissue culture of this cultivar and others may offer a massive cloned plant production that permit to satisfy the market demand. Multinodal explants of Leucadendron 'Safari Sunset' were established in vitro on a half strength Murashige and Skoog medium supplemented with 2.2 µM benzylaminopurine (BAP). Various antioxidant treatments were used to reduce the presence of phenolic compounds in culture. No important differences in growth and multiplication between these treatments were detected during in vitro establishment and multiplication, but explants cultured on a medium with 1 g L -1 of polyvinylpyrrolidone (PVP) developed a higher number of buds in the establishment phase. Proliferation of axillary shoots was achieved with 0.88 µM BAP, being slightly higher on a medium with 2.2 µM BAP although the buds were deformed, showing abnormal leaves. The use of phytagel in this phase increased proliferation rate and shoot growth. Low percentages rooting were obtained when microcuttings were cultured on the same basal medium supplemented with several indole-3-butyric acid (IBA) concentrations. The best rooting percentage (37.5%) was obtained with 44.1 µM IBA. This work shows that micropropagation of L. 'Safari Sunset' is possible with multinodal explants, although is necessary to optimize the protocol for commercial applications.Additional key words: antioxidant treatment, in vitro tissue culture, ornamental plants, phenolic compounds, Proteaceae. ResumenEmpleo de explantos multinodales para la micropropagación de Leucadendron 'Safari Sunset' Leucadendron 'Safari Sunset' es uno de los cultivares más importantes de Proteaceae debido a su importancia económica como flor cortada. El cultivo in vitro de este cultivar y otros, puede ofrecer una producción masiva de clones que permita satisfacer la demanda del mercado. Se establecieron in vitro explantos multinodales de L. 'Safari Sunset' en un medio Murashige y Skoog, con los macronutrientes a la mitad de su concentración, y bencilaminopurina (BAP) 2,2 µM. Para reducir la presencia de compuestos fenólicos en los cultivos, se emplearon diferentes tratamientos antioxidantes. No se observaron diferencias importantes en cuanto al crecimiento y multiplicación de las yemas, entre los antioxidantes empleados durante el establecimiento y multiplicación in vitro, sin embargo, se obtuvo un mayor nú-mero de yemas (25 ± 2,82) en los explantos sembrados en un medio con 1 g L -1 de polivinilpirrolidona (PVP). La multiplicación de las yemas se logró con BAP 0,88 µM. La tasa de proliferación fue ligeramente superior al adicionar al medio de cultivo BAP 2,2 µM, pero en este medio se desarrollaron yemas deformes, con hojas anormales. El empleo de fitagel incrementó la tasa de multiplicación y crecimiento de los vástagos. Se obtuvieron bajos porcentajes de enraizamiento al cultivar los microesquejes en el medio basal suplementado con diferentes concentraci...

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Propagation of Proteaceae

Cited by 23 publications

References 0 publications

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Effect of species, cutting position and exogenous rooting substances on rooting of honeybush (Cyclopiaspp.) cuttings

Use of multinodal explants for micropropagation of Leucadendron ‘Safari Sunset’

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