Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells

Nakauchi, Mina; Nagata, Noriyo; Takayama, Ikuyo; Saito, Shinji; Kubo, Hideyuki; Kaida, Atsushi; Oba, Kunihiro; Odagiri, Takato; Kageyama, Tsutomu

doi:10.3390/v11030216

Cited by 18 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others reported kinocilia on the apical surface of 3D tissue models on transwell inserts, Matrigel, or collagen gels using HBEC3-KT. 11,19,26 However, only little information on cilia functionality and surface coverage has been available. The SIS shows a connective tissue-like architecture made of mostly collagen and elastin.…”

Section: Discussionmentioning

confidence: 99%

Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

Lodes

Seidensticker

Perniss

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

Three-dimensional respiratory tissue models have been generated using, for example, human primary airway epithelial cells (hAEC) or respective cell lines. To investigate ciliopathies, such as primary ciliary dyskinesia, the presence of functional kinocilia in vitro is an essential prerequisite. Since access to hAEC of healthy donors is limited, we aimed to identify a respiratory epithelial cell line that is capable to display functional kinocilia on at least 60% of the apical surface. Thus, we cultured four different human respiratory cell lines with human primary airway fibroblasts under airlift conditions, characterized the morphology, and analyzed ciliary function. Only one of the tested cell lines showed beating kinocilia; however, <10% of the whole surface was covered and ciliary beating was undirected. Positive control tissue models using hAEC and fibroblasts displayed expected directed ciliary beating pattern around 11 Hz. Our data show that the available cell lines are not suitable for basic and applied research questions whenever functional kinocilia are required and that, rather, hAEC-or human induced pluripotent stem cell-derived tissue models need to be generated.To study ciliopathies or Bordetella pertussis infection in vitro, three-dimensional respiratory tissue models with functional kinocilia covering at least 60% of the model's surface are mandatory. We cultured four respiratory cell lines on a fibroblastloaded biological scaffold and showed that none of them met this requirement. In contrast, primary airway cell-derived models sufficiently reflected the mucociliary phenotype. To further search for an alternative to primary respiratory cells, investigations on other cell lines should be conducted or even new cell lines have to be generated. IntroductionT hree-dimensional (3D) cell cultures of the respiratory epithelium/mucosa afford research on-for examplethe impact of airborne pollutants, host-pathogen interactions, and drug permeation. 1-5 As source for respiratory epithelial cells, primary cells, induced pluripotent stem cells (iPSC), or cell lines can be used. Both primary cells and iPSC provide the opportunity to generate personalized tissue models, for example, to study individual drug responses or drug efficacy. Moreover, they show a high in vitro-in vivo correlation. These models feature a pseudostratified epithelial morphology, barrier properties, basal cells, mucus-producing goblet cells, and ciliated cells facilitating mucociliary clearance. [6][7][8][9]

show abstract

Section: Discussionmentioning

confidence: 99%

Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

Lodes

Seidensticker

Perniss

et al. 2020

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Abbreviations used GINA: Global Initiative for Asthma RV: Rhinovirus were previously able to identify species-specific antibody responses to RV-C-derived peptides, a species that has been difficult to propagate. 18 For the latter reason, we also know less about pathogenic differences between RV-A and RV-C strains in the etiology of asthma exacerbations because no infection models for RV-C are available. 19,20 However, it has been found that early multiple allergic sensitization in the first years of life is an important predictor of asthma.…”

Section: Follow-up Visitmentioning

confidence: 99%

Toward personalization of asthma treatment according to trigger factors

Niespodziana

Borochova

Pazderova

et al. 2020

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

Disclosure of potential conflict of interest K. Niespodziana receives grant support (grant no. P29398) from the Austrian Science Foundation (FWF). K. F. Chung has received honoraria for participating in advisory board meetings of GlaxoSmithKline (GSK), AstraZeneca, Novartis, Merck, Boehringer Ingelheim, and Teva regarding treatments for asthma and chronic obstructive pulmonary disease and has also been renumerated for speaking engagements. A. Custovic reports personal fees from Novartis,

show abstract

“…The continuous invasion and high diversity of rhinoviruses have been observed [ 138 ]. Rhinovirus activity has been detected in countries of all continents: Spain [ 139 ], Brazil [ 140 , 141 , 142 ], Argentina [ 143 ], France [ 125 ], Italy [ 10 , 144 ], the United Kingdom [ 116 ], Russia [ 145 ], Finland [ 146 , 147 ], Canada [ 148 ], the US [ 3 , 102 , 137 , 149 , 150 , 151 ], Australia [ 152 ], Vietnam [ 153 ], China [ 154 , 155 ], Hong Kong [ 156 ], Japan [ 157 ], Burundi [ 158 ], KwaZulu-Natal (a province of South Africa) [ 159 ], Tanzania [ 90 ], and many others, including Antarctica [ 160 ].…”

Section: Epidemiologymentioning

confidence: 99%

COVID-19 Shuts Doors to Flu but Keeps Them Open to Rhinoviruses

Kiseleva

Ksenafontov

2021

Biology

View full text Add to dashboard Cite

It is well known that rhinoviruses are distributed across the globe and are the most common cause of the common cold in all age groups. Rhinoviruses are widely considered to be harmless because they are generally perceived as respiratory viruses only capable of causing mild disease. However, they may also infect the lower respiratory tract, inducing chronic obstructive pulmonary disease and exacerbations of asthma, bronchiolitis, etc. The role of rhinoviruses in pathogenesis and the epidemiological process is underestimated, and they need to be intensively studied. In the light of recent data, it is now known that rhinoviruses could be one of the key epidemiological barriers that may influence the spread of influenza and novel coronaviruses. It has been reported that endemic human rhinoviruses delayed the development of the H1N1pdm09 influenza pandemic through viral interference. Moreover, human rhinoviruses have been suggested to block SARS-CoV-2 replication in the airways by triggering an interferon response. In this review, we summarized the main biological characteristics of genetically distinct viruses such as rhinoviruses, influenza viruses, and SARS-CoV-2 in an attempt to illuminate their main discrepancies and similarities. We hope that this comparative analysis will help us to better understand in which direction research in this area should move.

show abstract

Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells

Cited by 18 publications

References 30 publications

Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

Investigation on Ciliary Functionality of Different Airway Epithelial Cell Lines in Three-Dimensional Cell Culture

Toward personalization of asthma treatment according to trigger factors

COVID-19 Shuts Doors to Flu but Keeps Them Open to Rhinoviruses

Contact Info

Product

Resources

About