Propagation of saffron (<i>Crocus sativus</i> L.) through tissue culture: a review

Tahiri, Abdelghani; Mazri, Mouaad Amine; Karra, Youssef; Aabd, Naima Ait; Bouharroud, Rachid; Mimouni, Abdelaziz

doi:10.1080/14620316.2022.2078233

Cited by 12 publications

(6 citation statements)

References 92 publications

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“…The absence of callus in the basal parts of the regenerants is very fruitful in adaptation because the vascular system of the root and the vascular system of the plantlet are better connected. Although the effect of different basal media on the regenerative ability of in vitro cultured plants has been investigated in numerous studies (Kairuz et al 2021;Tahiri et al 2022;Pullaiah et al 2022), there have been no reports on the effectiveness of basal media in bulbous plants under in vitro conditions. The use of ve different media in this study showed that some media, including N6, B5, and WPM, limited the ability of explants.…”

Section: Discussionmentioning

confidence: 99%

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

Zare

Rabiei

Mohammadi

2023

Preprint

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Allium jesdianum Boiss. & Buhse. is a pharmaceutically valuable plant that is threatened with extinction due to over-exploitation of wild plants. In vitro culture of plants is a favorable alternative for the conservation of endangered plant species. To establish an efficient protocol for micropropagation of Allium jesdianum, the effects of plant growth regulators, basal medium, and light were studied in this experiment. Basal parts of collected bulbs from Zagros Mountains in Iran were surface sterilized and placed on five different basal media with different concentrations of IBA, NAA, BAP and kinetin and kept under dark and light conditions. The results showed that among the exogenously applied PGRs, IBA at concentrations of 1 and 2 mg/l was the most convincing PGR for shoot and bulblet regeneration. The best results were obtained with CLC and MS media under light. Higher NH4+ also increased regeneration. Our experiment may have implications for plant growth and conservation, as well as for in vitrobreeding and drug development.

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Section: Discussionmentioning

confidence: 99%

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

Zare

Rabiei

Mohammadi

2023

Preprint

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“…Many papers that have reported on in vitro saffron propagation come from several countries [5,7,[38][39][40][41] and some reviews [42][43][44] have collected all the information on this fundamental method for propagating the Crocus spp. [2], especially in C. sativus, to develop callus or new shoot buds and obtain new microcorms formation.…”

Section: In Vitro Propagation Of Saffronmentioning

confidence: 99%

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Tarraf,

İzgü,

Şimşek

et al. 2024

Horticulturae

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The propagation of Crocus sativus L. relies exclusively on corm multiplication. As underground storage organs, corms are susceptible to a wide range of pathogens, environmental stresses, and diseases, making traditional propagation methods often ineffective with the loss of valuable material. In vitro propagation offers an alternative for the saffron culture under controlled conditions. In particular, the innovative application of the Temporary Immersion System (TIS) represents a technological advancement for enhancing biomass production with a reduction in operational costs. The current study utilized the Plantform™ bioreactor to propagate in vitro saffron corms from the ‘Abruzzo’ region (Italy), integrating machine learning models to assess its performance. The evaluation of saffron explants after 30, 60, and 90 days of culture showed a marked improvement in growth and microcorm production compared to conventional in vitro culture on semisolid medium, supported by the machine learning analysis. Indeed, the Random Forest algorithm revealed a predictive accuracy with an R2 value of 0.81 for microcorm number, showcasing the capability of machine learning models to forecast propagation outcomes effectively. These results confirm that applying TIS in saffron culture could lead to economically viable, large biomass production within a controlled environment, irrespective of seasonality. This study represents the first endeavor to use TIS technology to enhance the in vitro propagation of saffron in conjunction with machine learning, suggesting an innovative approach for cultivating high-value crops like saffron.

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“…Saffron production relies on traditional, labor-intensive methods [8], creating job opportunities, especially for women and young individuals [9]. Despite its adaptability to marginal land and low-input farming systems [10], challenges such as a short flowering season, modest yields, manual harvesting, and limited global distribution impact farmers' income [9].…”

Section: Introductionmentioning

confidence: 99%

Energy, Environmental, and Economic Sustainability of Saffron Cultivation: Insights from the First European (Italian) Case Study

Mehmeti,

Candido,

Canaj

et al. 2024

Sustainability

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Saffron (Crocus sativus L.) stands as a valuable agricultural commodity, witnessing an increasing market inclination toward environmentally sustainable and eco-friendly products. The current literature on the environmental impact and profitability of saffron cultivation is limited, underscoring a notable gap in comprehending the sustainability aspects of this crop. This study utilized a comprehensive multi-model approach to assess the sustainability of annual saffron cultivation, representing the first global detailed evaluation, conducted within a European context (Southern Italy). Energy analysis, physical and monetized life cycle assessment (LCA), and life cycle costing (LCC) were used for a cradle-to-farm gate assessment. One hectare of cultivated saffron, one saffron production yield (stigma, corm, and flower), and 1 kg of stigma yield were used as functional units. The total energy input was 65,073 MJ ha−1, being 33% direct, 67% indirect, 72% renewable, and 28% non-renewable. The majority (55%) of energy is derived from corm production. For 1 kg of saffron the energy efficiency, specific energy, and productivity were 2.98, 4.64 MJ kg−1, and 0.22 kg MJ−1, respectively, while these values dropped significantly for 1 kg of stigma. The multi-indicator LCA analysis using the ReCiPe 2016 model revealed significant contributions to various environmental impact categories. Results align with prior research, pinpointing fertilization and mechanical operations as the primary drivers of diverse environmental impacts. A noticeable carbon intensity was estimated, with a relevant contribution from corm production and human labor, aspects overlooked in previous LCA studies. Saffron cultivation maintains economic viability, with production costs at EUR 98,435 per ha−1 and a net return margin of EUR 172,680 per ha−1, bolstered by the high market price and by-product revenue. Monetization of LCA results revealed that external costs were EUR 15,509 per ha−1, being only 14% of the total cost. Investments in improving yield and resource efficiency have the potential to increase the eco-efficiency of saffron cultivation.

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Propagation of saffron (Crocus sativus L.) through tissue culture: a review

Cited by 12 publications

References 92 publications

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Energy, Environmental, and Economic Sustainability of Saffron Cultivation: Insights from the First European (Italian) Case Study

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Propagation of saffron (Crocus sativus L.) through tissue culture: a review

Cited by 12 publications

References 92 publications

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. &amp; Buhse

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. &amp; Buhse

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Energy, Environmental, and Economic Sustainability of Saffron Cultivation: Insights from the First European (Italian) Case Study

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Product

Resources

About

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse

The effect of plant growth regulators, basal medium and light on micropropagation of Allium jesdianum Boiss. & Buhse