Propagation of the Fern Adiantum Capillus-Veneris Through Tissue Culture of the Circinate Part of Young Leaves

Salomé, M.; Pais, S.; Casal, M.

doi:10.17660/actahortic.1987.212.109

Cited by 5 publications

(2 citation statements)

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“…Although there are several literatures on the direct SE of A. capillus-veneris , few of them have presented sufficient histological evidence to describe this process (Salomé et al, 1985; Amaki and Higuchi, 1991; Somer et al, 2010). In addition, the genes required to regulate callus and GGB induction and development are not well defined in A. capillus-veneris .…”

Section: Introductionmentioning

confidence: 99%

“…As a leptosporangiate fern, Adiantum capillus-veneris has become the subject of many cytological, developmental, physiological, and phylogenetic studies (Pryer et al, 2001 ; Wada, 2007 ; Xie et al, 2008 ; Li et al, 2013 ). Although there are several literatures on the direct SE of A. capillus-veneris , few of them have presented sufficient histological evidence to describe this process (Salomé et al, 1985 ; Amaki and Higuchi, 1991 ; Somer et al, 2010 ). In addition, the genes required to regulate callus and GGB induction and development are not well defined in A. capillus-veneris .…”

Section: Introductionmentioning

confidence: 99%

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Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro: New Insights into the Evolution of Reproductive Organs in Land Plants

Li¹,

Han

Fang

et al. 2017

Front. Plant Sci.

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An efficient in vitro regeneration system via somatic embryogenesis (SE) was developed for a fern species Adiantum capillus-veneris. Adventitious shoots, green globular bodies (GGBs) and calli were obtained with the maximal induction rate on the Murashige and Skoog (MS) medium of low concentrations of 6-benzyladenine (BA) (0–1.0 mg/L), 2.0 mg/L BA without 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 2,4-D and 0.5–1.0 mg/L 6-BA, respectively. Cyto-morphological and histological changes in the shoot development via calli and GGBs were examined. For a better understanding of these developmental events, expression patterns of six genes, AcLBD16, AcAGL, AcBBM, AcWUS, AcRKD, and AcLEC1, were characterized during SE. AcBBM and AcLEC1 were ubiquitously expressed in direct SE (adventitious shoots and GGBs) the maximal expression of AcBBM in mature GGBs, and the high expression of AcLEC1 in GGB initiation and adventitious shoots. During the indirect SE, AcLBD16, AcLEC1, AcRKD, and AcWUS were highly expressed in mature calli. Additionally, phylogenetic analyses showed that AcWUS, AcBBM, AcLBD, AcAGL, AcRKD, and their homologs of other green plants formed monophyletic clades, respectively. Some of these gene families, however, diversified rapidly with the occurrence of embryophytes, suggesting that embryogenesis-associated genes could experience a rapid evolution with the colonization of plants to terrestrial environments. Expression and phylogenetic analyses of those embryogenesis-associated genes by the aid of in vitro regeneration system of A. capillus-veneris provide new insights into the evolution of reproductive organs in land plants.

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Section: Introductionmentioning

confidence: 99%