Propagation of the Immune Response by Cell Transfer

Wakefield, June D.; Nr, Rose

doi:10.1159/000230290

Cited by 1 publication

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“…The concentration of leukocytes averages roughly 1 x 10' cells/ml in an immunized animal and is about half as great when adjuvant alone is employed. The major difference, as compared to methods described by others (e.g., [18][19][20][21]), is the large volume of fluid obtained and the relatively high concentration of lymphocytes. In addition, antibodies as well as cells can be isolated from the ascites.…”

Section: Discussionmentioning

confidence: 96%

Transfer of idiotype suppression and idiotypes by leukocytes from ascitic fluids

Satō

Nisonoff

1977

Eur J Immunol

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A procedure is described for producing ascites containing both high concentrations of antibodies and immunologically active leukocytes. In mice that were immunologically suppressed with respect to a cross-reactive idiotype, the ascitic cells were found to be very active in adoptively transferring the suppressed state to mildly irradiated, syngeneic recipients and simultaneously transferring the capacity to produce the "private" idiotype characteristic of the donor's anti-p-azophenylarsonate antibodies. The participation of both T and B cells is therefore suggested. As few as 2 x 10(6) cells were sufficient for suppression and for inducing the production of the private idiotype in the recipient, in concentrations comparable to that of the donor mouse. A single donor can thus be used to colonize many recipients with the private idiotype, without sacrifice of the donor. The method should be useful in structural studies of antibodies of a given specificity with different idiotypes, and for investigations of the mechanism of idiotype suppression, as well as other studies in cellular immunology.

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Section: Discussionmentioning

confidence: 96%