Propagation of Tulip in Vitro

Kuijpers, Anne-Marie; Langens-Gerrits, Merel

doi:10.17660/actahortic.1997.430.49

Cited by 20 publications

(10 citation statements)

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“…Generally, chilled explants formed adventitious bulblets on media containing 0.5 mgL -1 TDZ + 0.3 mg L -1 NAA kept in the dark (Figure 4E). Our observation confirms previous findings of other studies that the induction of tulip bulblets occurred in darkness after seed cultivation at 5°C during 10-12 wk period (Kuijpers and Langens-Gerrits, 1997). A -embryo at the stage of excision (52-54 DAA); B -differentiation of embryo organs after 10 days of cultivation on Variant 1 medium; C, D -the formation of dropper (dr) and cotyledon leaf (cl); E -the initial stages of bulbs formation (b) on Variant 1B medium after exposure to light and temperature of 20°C followed by chilling at 4°C for 12 wk; F -appearance of fasciated cotyledon leaves developed under influence of high temperature and TDZ addition to the initial medium (Variant 2B); G -haustorium-like region in the base of cotyledon leaf; H -shoot cultures initiated after 8 wk maintenance on multiplication medium.…”

Section: The Pathways Of Embryo Development In Vivo and In Vitrosupporting

confidence: 93%

“…A period of low temperature is required to interrupt the embryo dormancy, induce germination and initiate bulb primordium (Khodorova and Boitel-Conti, 2013). Furthermore, it was reported that the in vitro formation of bulblets from tulip was induced by cultivation at 20°C for 14 to 18 wk (Maslanka and Bach, 2014), while in another study the regenerated shoots were able to form bulblets only after cold treatment for 12 wk (Kuijpers and Langens-Gerrits, 1997).…”

Section: Introductionmentioning

confidence: 93%

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The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions

Yurievna

Vladimirovna

2019

Ornam. Hortic.

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Section: The Pathways Of Embryo Development In Vivo and In Vitrosupporting

confidence: 93%

Section: Introductionmentioning

confidence: 93%

The application of different reproduction techniques for rare species waterlily tulip (Tulipa kaufmanniana Regel.) propagation under ex situ conditions

Yurievna

Vladimirovna

2019

Ornam. Hortic.

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“…But it was generally lower than that noted in explants derived from non-cooled bulbs. Replacement of the most commonly used cytokinins (BAP and 2iP) with zeatin (1 mg l -1 ) enhanced regeneration capacity (Hulscher et al 1992;Kuijpers and Langens-Gerrits 1997). Moreover, the latter authors found that application of methyljasmonate or paclobutrazol during the initial stage improved meristem formation in regenerants.…”

Section: Introductionmentioning

confidence: 94%

Effects of thidiazuron and paclobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication

Podwyszyńska

Marasek

2011

Acta Soc Bot Pol

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The effects of TDZ and paclobutrazol on the primary regeneration on tulip flower stalk explants of six cultivars and subsequent shoot multiplication were examined. Explants, flower stalk slices, were excised from cooled and subsequently forced bulbs. The explants were incubated for two months in darkness on medium containing NAA and cytokinins, 2iP and BAP, as control, or TDZ (0.5-4 mg l -1 ) and paclobutrazol (0.05-0.4 mg l -1 ). Then, the regenerating explants were subcultured on medium with TDZ and NAA applied at low concentrations. Different regeneration capabilities were found depending on cultivar and growth regulators. The percentage of explants forming leaf-like structures ranged, on the control medium, from 80% in Blue Parrot and Prominence to below 30% in Apeldoorn and Mirjoran. TDZ, applied at optimum for each cultivar concentration, greatly increased the regeneration potential up to 70-100%. Paclobutrazol, added to the TDZ-containing medium, significantly enhanced the response of explants, resulting in high numbers of leaf-like structures formed per explant (13.7--22.8). The structures developed gradually into characteristic forms: the growing up cotyledonary leaf, the probable root primordium formed at its base, the growing downwards stolon and the shoot meristem developed finely on its tip. It is suggested that such primary regeneration may have a nature of somatic embryogenesis. Then, the adventitious shoots developed and formed clusters, which were divided into 2-3 smaller ones every two months. The growth regulators, used at initial stage, markedly influenced subsequent shoot multiplication. Thus, the most intensive shoot formation was noted with TDZ at concentrations of 0.5-2 mg l -1 and paclobutrazol of 0.05-0.1 mg l -1 .

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“…al. 1992;Kuijpers and Langens-Gerrits, 1997;Le Nard and Chanteloube, 1992;Podwyszyńska, 2003) or immature embryos isolated from mature seeds (Famelaer, 1996) has been reported, but the propagation factor and the growth rate were still low. The most convenient way of regeneration is through somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%