Propagation Protocol of the Medicinal Plant – Aloe Vera Using Tissue Culture

Abstract

Aloe vera is one of the most popular cactus-type plants in the global market due to its widespread uses in pharmaceutical, cosmetic, food, and decorative purposes. The present study derived callus cultures from the Aloe vera plant leaves, then reproduced on agar-solidified MS medium from June to December 2021 at the University of Mosul, Iraq. Results revealed that the MS medium + 3.0 mg L-1 benzyl adenine (BA) proved suitable for induction of leaf callus up to 85%, while the MS medium supplement with 1.0 and 2.0 mg L-1 BA reached 70%. The MS medium with 1.0 mg L-1 BA showed the best results for growing apical shoots of A. vera plants and producing vegetative branches. The formation of roots emerged within two weeks after placing them on the rooting medium. The shoots regenerated from the growing apices and were rooted easily in agar-solidified MS medium. The obtained plants attained successful acclimatization in terms of their growth and length, afterward, transferred to the peat-moss mixture.

Advancing the Production and Health benefits of Aloe Barbadensis Miller Through Biotechnology Enabled Integration of Pathology and Breeding Approaches

“…The epidermis and the algae are present beneath the thick coating covering the leaves. Later is separated Journal of Survey in Fisheries Sciences 11 (1) 49-56 2024 51 between lower tissue and higher chlorenchyma; as the rosette ages, the subsequent leaves become grey-green and contain fewer whitish dots. Every 7-8 weeks, the plant can be collected by removing 3-5 leaves from each plant.…”

Genetic Relationship Among Some Genera of the Family Cactaceae

“…The random amplified polymorphic DNA (RAPD) markers have become more popular for analyzing the genetic diversity of different species. Nowadays, molecular markers have shown effectiveness in determining the genetic diversity among and within species (Charcosset and Moreau, 2004;Al-Nema and Abdullah, 2023;Mohsin et al, 2023). These molecular markers might be employed as supplementary approaches to morphological descriptors to show their promise (Demey et al, 2003).…”