Propanols

Papa, Anthony J.

doi:10.1002/14356007.a22_173

Cited by 26 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2-Propanol is used primarily as a solvent in inks and surfactants. Further applications include its uses as an antiseptic alcohol, as a reaction solvent for cellulose carboxymethyl ether, in the production of cosmetic base materials and pesticide carriers, and for removal of water from gasoline tanks in cars ( Papa, 2011 ). Banerjee et al (2014) used a lactose-inducible expression system to control ASO construct, CO as substrate in uncontrolled batch experiments, and achieved up to 15 mM of 2-propanol.…”

Section: Discussionmentioning

confidence: 99%

Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis

et al. 2016

View full text Add to dashboard Cite

Synthesis gas (syngas) fermentation by anaerobic acetogenic bacteria employing the Wood–Ljungdahl pathway is a bioprocess for production of biofuels and biocommodities. The major fermentation products of the most relevant biocatalytic strains (Clostridium ljungdahlii, C. autoethanogenum, C. ragsdalei, and C. coskatii) are acetic acid and ethanol. A comparative metabolic and genomic analysis using the mentioned biocatalysts might offer targets for metabolic engineering and thus improve the production of compounds apart from ethanol. Autotrophic growth and product formation of the four wild type (WT) strains were compared in uncontrolled batch experiments. The genomes of C. ragsdalei and C. coskatii were sequenced and the genome sequences of all four biocatalytic strains analyzed in comparative manner. Growth and product spectra (acetate, ethanol, 2,3-butanediol) of C. autoethanogenum, C. ljungdahlii, and C. ragsdalei were rather similar. In contrast, C. coskatii produced significantly less ethanol and its genome sequence lacks two genes encoding aldehyde:ferredoxin oxidoreductases (AOR). Comparative genome sequence analysis of the four WT strains revealed high average nucleotide identity (ANI) of C. ljungdahlii and C. autoethanogenum (99.3%) and C. coskatii (98.3%). In contrast, C. ljungdahlii WT and C. ragsdalei WT showed an ANI-based similarity of only 95.8%. Additionally, recombinant C. ljungdahlii strains were constructed that harbor an artificial acetone synthesis operon (ASO) consisting of the following genes: adc, ctfA, ctfB, and thlA (encoding acetoacetate decarboxylase, acetoacetyl-CoA:acetate/butyrate:CoA-transferase subunits A and B, and thiolase) under the control of thlA promoter (PthlA) from C. acetobutylicum or native pta-ack promoter (Ppta-ack) from C. ljungdahlii. Respective recombinant strains produced 2-propanol rather than acetone, due to the presence of a NADPH-dependent primary-secondary alcohol dehydrogenase that converts acetone to 2-propanol. Furthermore, the ClosTronTM system was used to construct an adhE1 integration mutant. These results provide extensive insights into genetic features of industrially relevant bacterial biocatalysts and expand the toolbox for metabolic engineering of acetogenic bacteria able to ferment syngas.

show abstract

Section: Discussionmentioning

confidence: 99%

Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Following the evaluation, adjustments to the construction of DCLs are made and often involve: replacement of some of the constituents within DCLs, alteration of the reversible chemistry used, a change in the external stimuli applied. For instance, in Hanai et al (2007) authors report the use of metabolic engineering in designing a synthetic pathway in E. coli for the production of isopropanol (Papa, 2005), a secondary alcohol that is used, among other ways, in pharmaceutical applications. The successful strain was developed through expression of a variety of combinations of genes from a selected list of known strains.…”

Section: Methodsmentioning

confidence: 99%

Alternative methods of processing bio-feedstocks in formulated consumer product design

2014

View full text Add to dashboard Cite

In this work new methods of processing bio-feedstocks in the formulated consumer products industry are discussed. Our current approach to formulated products design is based on heuristic knowledge of formulators that allows selecting individual compounds from a library of available materials with known properties. We speculate that most of the compounds (or functions) that make up the product to be designed can potentially be obtained from a few bio-sources. In this case, it may be possible to design a sequence of transformations required to convert feedstocks into products with desired properties, analogous to a metabolic pathway of a complex organism. We conceptualize some novel approaches to processing bio-feedstocks with the aim of bypassing the step of a fixed library of ingredients. Two approaches are brought forward: one making use of knowledge-based expert systems and the other making use of applications of metabolic engineering and dynamic combinatorial chemistry.

show abstract

“…This firm is focused on mixotrophic fermentation (at pilot scale) using sugars and syngas/CO to produce mainly acetone and isopropanol, although it also envisions a larger portfolio of products derived from acetyl‐CoA . Acetone is widely employed as a solvent and as an intermediate to methyl methacrylate and bisphenol A, both of which are used in polymers’ manufacturing, while isopropanol is mainly a solvent in inks and surfactants . Besides White Dog Labs, LanzaTech has also demonstrated the production of acetone and isopropanol…”

Section: Biochemical Routes Using C1 Feedstocksmentioning

confidence: 99%

Gas fermentation of C1 feedstocks: commercialization status and future prospects

Teixeira

Moutinho

Romão‐Dumaresq

2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

The increasing emissions of carbon dioxide, methane and carbon oxide (collectively referred as C1 compounds) are likely to configure a major contribution to global warming and other environmental issues. The implementation of carbon capture and storage (CCS) is considered a crucial strategy to prevent global warming, but the overall costs of currently available CCS technologies are still prohibitive for its large‐scale deployment. Using microorganisms capable of assimilating C1 compounds for producing value‐added products could be an important driver for mitigating emissions and minimizing their deleterious consequences, while simultaneously deriving additional economic benefits from these compounds. This review summarizes the main microorganisms and metabolic routes being investigated, with special focus on both the products targeted and the current industrial initiatives. There are a number of companies investing in these routes and in some instances commercial deployment was identified. Despite the variety of commercially‐appealing products, genetic manipulation of microorganisms to maximize yields and the design of technologies capable of efficiently using the gaseous feedstocks are major challenges yet to be overcome to fully unlock the potential of C1 microbiological routes. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Propanols

Cited by 26 publications

References 13 publications

Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis

Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis

Alternative methods of processing bio-feedstocks in formulated consumer product design

Gas fermentation of C1 feedstocks: commercialization status and future prospects

Contact Info

Product

Resources

About