Properties and binding forms of cadmium and nickel in protein extracts from bean seeds (Phaseolus vulgaris L.)

Lange-Hesse, Kirsten; Dunemann, Lothar; Schwedt, Georg

doi:10.1007/bf00322934

Cited by 21 publications

(2 citation statements)

References 19 publications

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“…In recent decades many studies have described the chemical forms and qualitative binding properties of heavy metals in food. These studies were mainly focused on the properties and binding forms of heavy metals in bean seeds (1)(2)(3), Lentinue edodes (4), and some other food products (5). Some studies on the quantitative distribution and chemical forms of heavy metals in rice grains were also described (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

THE PATTERNS OF Cd-BINDING PROTEINS IN RICE AND WHEAT SEED AND THEIR STABILITY

Wong

Yang

2002

Journal of Environmental Science and Health, Part A

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The protein-binding forms of cadmium in polluted rice and wheat seeds and their stability were investigated using the methods of Sephadex chromatography. Three absorption peaks (F-I, F-II and F-III) were identified in Tris-HCl extraction of rice and wheat on Sephadex G 75. The Cd in the protein extracts from rice and wheat seeds was distributed mainly in the fractions of F-I and F-III. The apparent molecular weights of Cd-binding proteins for F-I and F-III were 54.5 and 5.5 KD, respectively. The components of amino acid for the protein bound with heavy metals were different. There were high contents of glutamic acid, cysteine, valine, isoleucine, leucine and tyrosine in the protein extracts of rice and wheat. After cooking, the Cd-binding proteins were destroyed. High molecular weight protein-binding form (54.5 KD) was broken into low molecular weight complex (5.5 KD) or tiny peptide chain. Simultaneously, Cd bound with protein was released, or mainly bound with protein of smaller molecular size. Enzyme treatment (pepsin and trypsin) also caused a destruction of Cd binding protein and a change in the distribution of Cd in the eluent. The concentrations of Cd in the elution of first and third peak decreased markedly, and the Cd distribution was observed in the elution after third peak (F-III).

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Section: Introductionmentioning

confidence: 99%

THE PATTERNS OF Cd-BINDING PROTEINS IN RICE AND WHEAT SEED AND THEIR STABILITY

Wong

Yang

2002

Journal of Environmental Science and Health, Part A

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“…The effects of Ni on the uptake of other nutrients have already been studied in adult plants and seedlings (Cataldo et al, 1978;Gabbrielli et al, 1991;Kovačević et al, 1999), however there have been few studies of germinating seeds. The assimilation, concentration and distribution of heavy metals in seeds have been explored in species used by the food industry (Lange-Hesse et al, 1994;He et al, 2000;Mengchang et al, 2000), but there have been few studies of the seeds of serpentine species (Psaras and Manetas, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of Three Nickel Salts on Germinating Seeds of Grevillea exul var. rubiginosa, an Endemic Serpentine Proteaceae

Léon¹,

Rabier²,

Notonier³

et al. 2005

Annals of Botany

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Flame and Graphite Furnace Atomic Absorption Spectrometry in Environmental Analysis

Sperling¹

2000

Encyclopedia of Analytical Chemistry

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Atomic absorption spectrometry (AAS) is one of the most often used techniques for the quantitative determination of elements in environmental materials at trace and ultratrace levels. AAS is an optical atomic spectrometric technique based on the measurement of the specific absorption originating from free nonionized atoms in the gas phase. To transfer the analyte to free atoms, different types of atomizer are in use, the flame and the graphite furnace types being the most often used. Typical detection limits of flame atomic absorption spectrometry (FAAS) are of the order of 1–100 µg L −1 , making it a perfect tool for the determination of minor and trace elements, at least for contaminated samples. Graphite furnace atomic absorption spectrometry (GFAAS), offering detection limits which are about a factor of 20–200 lower than for FAAS, is the standard method for many trace elements, especially for background values, and for unpolluted samples, such as fresh water and biological materials. AAS in its conventional configuration is a single‐element technique, which has to be used in a sequential mode when more than one element has to be determined. However, there are commercial instruments available that can be used for the determination of 6–8 elements simultaneously.

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Properties and binding forms of cadmium and nickel in protein extracts from bean seeds (Phaseolus vulgaris L.)

Cited by 21 publications

References 19 publications

THE PATTERNS OF Cd-BINDING PROTEINS IN RICE AND WHEAT SEED AND THEIR STABILITY

THE PATTERNS OF Cd-BINDING PROTEINS IN RICE AND WHEAT SEED AND THEIR STABILITY

Effects of Three Nickel Salts on Germinating Seeds of Grevillea exul var. rubiginosa, an Endemic Serpentine Proteaceae

Flame and Graphite Furnace Atomic Absorption Spectrometry in Environmental Analysis

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