Properties and mechanism of the antimicrobial peptide APT produced by Lactobacillus ALAC-4

Wang, Shuai; Zhang, Yujiao; Mandlaa,; Sun, Ziyu; Chen, Zhongjun

doi:10.1016/j.lwt.2022.113713

Cited by 12 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, specimens are attached to metallic stubs using carbon stickers and sputter-coated with gold for 30 s by the Lon Sputtering Apparatus (HITACH MC1000, Tokyo, Japan). At last, the samples were observed by SEM (HITACHI Regulus 8100, 1 kV, Tokyo, Japan) [ 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities

Song,

Wang,

Liu

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

In recent years, there has been a growing interest in antimicrobial peptides as innovative antimicrobial agents for combating drug-resistant bacterial infections, particularly in the fields of biofilm control and eradication. In the present study, a novel cationic antimicrobial peptide, named LC-AMP-F1, was derived from the cDNA library of the Lycosa coelestis venom gland. The sequence, physicochemical properties and secondary structure of LC-AMP-F1 were predicted and studied. LC-AMP-F1 was tested for stability, cytotoxicity, drug resistance, antibacterial activity, and antibiofilm activity in vitro compared with melittin, a well-studied antimicrobial peptide. The findings indicated that LC-AMP-F1 exhibited inhibitory effects on the growth of various bacteria, including five strains of multidrug-resistant bacteria commonly found in clinical settings. Additionally, LC-AMP-F1 demonstrated effective inhibition of biofilm formation and disruption of mature biofilms. Furthermore, LC-AMP-F1 exhibited favorable stability, minimal hemolytic activity, and low toxicity towards different types of eukaryotic cells. Also, it was found that the combination of LC-AMP-F1 with conventional antibiotics exhibited either synergistic or additive therapeutic benefits. Concerning the antibacterial mechanism, scanning electron microscopy and SYTOX Green staining results showed that LC-AMP-F1 increased cell membrane permeability and swiftly disrupted bacterial cell membranes to exert its antibacterial effects. In summary, the findings and studies facilitated the development and clinical application of novel antimicrobial agents.

show abstract

Section: Methodsmentioning

confidence: 99%

LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities

Song,

Wang,

Liu

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The antimicrobial peptide called APT with 40.32 KDa from L. plantarum ALAC-4 was discovered to have strong antifungal activity by degrading cell wall and membrane in the pH range of 4-4.5, and the activity was lowered by the proteinase treatment ( Chen et al, 2018 ). It was also found to decrease phosphorus demand and respiratory enzyme activity in Candida albicans ( Wang et al, 2022 ).…”

Section: Biological Control Of Fusaria By Lactic Acid Bacteriamentioning

confidence: 99%

Fusarium biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria

Krishnan,

Nampoothiri,

Suresh

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Mycotoxins produced by Fusarium species are secondary metabolites with low molecular weight formed by filamentous fungi generally resistant to different environmental factors and, therefore, undergo slow degradation. Contamination by Fusarium mycotoxins in cereals and millets is the foremost quality challenge the food and feed industry faces across the globe. Several types of chemical preservatives are employed in the mitigation process of these mycotoxins, and they help in long-term storage; however, chemical preservatives can be used only to some extent, so the complete elimination of toxins from foods is still a herculean task. The growing demand for green-labeled food drives to evade the use of chemicals in the production processes is getting much demand. Thus, the biocontrol of food toxins is important in the developing food sector. Fusarium mycotoxins are world-spread contaminants naturally occurring in commodities, food, and feed. The major mycotoxins Fusarium species produce are deoxynivalenol, fumonisins, zearalenone, and T2/HT2 toxins. Lactic acid bacteria (LAB), generally regarded as safe (GRAS), is a well-explored bacterial community in food preparations and preservation for ages. Recent research suggests that LAB are the best choice for extenuating Fusarium mycotoxins. Apart from Fusarium mycotoxins, this review focuses on the latest studies on the mechanisms of how LAB effectively detoxify and remove these mycotoxins through their various bioactive molecules and background information of these molecules.

show abstract

Fermentation of waste water from agar processing with Bacillus subtilis by metabolomic analysis

Wu,

Duan,

Lin

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Properties and mechanism of the antimicrobial peptide APT produced by Lactobacillus ALAC-4

Cited by 12 publications

References 36 publications

LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities

LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities

Fusarium biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria

Fermentation of waste water from agar processing with Bacillus subtilis by metabolomic analysis

Contact Info

Product

Resources

About