Properties of a bovine collagen type I membrane for guided bone regeneration applications

Abstract

Dental implant treatment requires an available bone volume in the implantation site to ensure the implant’s mechanical stability. When the bone volume is insufficient, one must resort to surgical means such as guided bone regeneration (GBR). In GBR surgery, bone grafts and membranes are used. The objective of this work is to manufacture and characterize the in vitro and in vivo properties of resorbable collagen type I membranes (Green Membrane®) for GBR. Membrane surface morphology was characterized by SEM and roughness was measured using an interferometric noncontact 3D system. In vivo skin sensitization and toxicity tests have been performed on Wistar rats. Bone defects were prepared in 24 adult male rats, filled with biomaterials (Blue Bone® and Bio Oss®) and covered with collagen membranes to maintain the mechanical stability of the site for bone regeneration. The incisions were closed with simple stitches; and 60 days after the surgery, the animals were euthanized. Results showed that the analyzed membrane was homogeneous, with collagen fiber webs and open pores. It had no sign of cytotoxicity and the cells at the insertion site showed no bone morphological changes. There was no tissue reaction and no statistical difference between Blue Bone® and Bio Oss® groups. The proposed membrane has no cytotoxicity and displays a biocompatibility profile that makes it suitable for GBR.

Ultrastructural and Physicochemical Characterization of a Non-Crosslinked Type 1 Bovine Derived Collagen Membrane

“…Brum et al [ 7 ] analyzed the cytotoxic, ultrastructural characteristics of a 1-mm thick non-reticulated collagen membrane indicated for guided bone regeneration surgery. The authors observed that the morphology of the membrane promotes an increase in the differentiation of osteocytes [ 7 ].…”

“…Brum et al [ 7 ] analyzed the cytotoxic, ultrastructural characteristics of a 1-mm thick non-reticulated collagen membrane indicated for guided bone regeneration surgery. The authors observed that the morphology of the membrane promotes an increase in the differentiation of osteocytes [ 7 ]. A systematic review and meta-analysis work on guided bone regeneration concluded that the association of the membrane improves the performance of the biomaterial and promotes an increase in the survival of implants [ 27 ].…”

“…Collagen membranes may be crosslinked or non-crosslinked. Collagen fiber organization is the most efficient way to improve the efficiency of non-crosslinked membranes [ 7 ]. A series of analyses can be used to investigate the properties of non-crosslinked collagen membranes and their biological effects [ 8 ].…”

Combining Sandblasting, Alkaline Etching, and Collagen Immobilization to Promote Cell Growth on Biomedical Titanium Implants

“…Collagen accounts for roughly 95% of the organic matrix in bone tissue and plays important roles in a variety of osteoblast activities [ 19 ], such as tissue repair through the activation of platelets via the integrin α2β1-binding domain [ 20 ]. These molecules also function as mediators of osteoblast cell responses [ 21 ], including in proliferation and migration during the healing process [ 22 , 23 , 24 ] as well as in secretion of the extracellular matrix (ECM) and osteoblast differentiation [ 21 ].…”

Osteopontin and Vascular Endothelial Growth Factor-Immunoreactivity in Critical Bone Defects Matrix Production: A Nano-Hydroxyapatite/Beta-Tricalcium Phosphate and Xenogeneic Hydroxyapatite Comparison

“…Recently, nanotechnology has been improving the bioactive properties of hydroxyapatite in relation to its crystals, resulting in a better response in association with nanocrystals than with thicker crystals [6]. The crystallinity is the property responsible for the biomaterial remaining for more or less time in the body, and with these advances in the way the crystals are manufactured, it is possible to control not only the reabsorption time but also the replacement of the material more efficiently [7][8][9].…”