Properties of a Cucumber Mosaic Virus Strain Naturally Infecting Chrysanthemum in India

Srivastava, K. M.; Raj, S. K.; Singh, B. P.

doi:10.1094/pd-76-0474

Cited by 30 publications

(11 citation statements)

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“…Serological tests Ouchterlony's double diffusion tests using crude antigen from naturally infected R. communis plants were performed as described earlier (Srivastava et al 1992). The wells were charged with antigen and antiserum with antiserum of CMV (PVAS 242a, ATCC, Rockville, MD, USA) and incubated overnight at room temperature (25±2°C) in a humid chamber and viewed on a light box.…”

Section: Methodsmentioning

confidence: 99%

First report of association of Cucumber mosaic virus with blister and leaf distortion of castor bean (Ricinus communis) in India

et al. 2010

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The association of Cucumber mosaic virus (CMV) with blister and leaf distortion symptoms of castor bean (Ricinus communis L.) has been detected by Ouchterlony's double diffusion tests and Western blot immunoassay using antiserum of CMV and confirmed by reverse transcription polymerase chain reaction (RT-PCR) using the coat protein gene specific primers of CMV which resulted in positive amplification of the expected size ∼650 bp band. The sequence analysis of RT-PCR amplicon from R. communis revealed 96-98% identities and close phylogenetic relationships with several isolates of the IB subgroup of CMV. The virus associated with blister and leaf distortion symptoms of castor bean was therefore identified as an isolate of CMV. The subgroup was also confirmed by RFLP analysis using a defined member of the IB subgroup of CMV. This is the first record of the natural occurrence of CMV on R. communis.

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Section: Methodsmentioning

confidence: 99%

First report of association of Cucumber mosaic virus with blister and leaf distortion of castor bean (Ricinus communis) in India

et al. 2010

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“…Somerville et al, (1987) observed local chlorotic lesions on cotyledonary leaves and epinasty on young true leaves of cucurbit hosts after mechanical inoculation with CMV-Py potato isolate and CMV-C pepper isolate of CMV. Srivastava et al, (1992) reported mosaic, vein clearing and necrotic local lesions type of symptoms on Cucumis sativus (Long Green) as a result of mechanical inoculation with a Chrysanthemum strain of CMV. All these findings along with our experimental outcome suggest that CMV can be mechanically transferred from infected potato to cucurbits.…”

Section: Aphid Population Dynamicsmentioning

confidence: 99%

Occurrence of Cucumber mosaic virus on Potato and its Transmission to Muskmelon under Potato-Cucurbit Cropping Pattern Followed in Punjab, India

Ghorai¹,

Kang²,

Sharma³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Broad host range of CMV from subgroup I and II have been identified in India, which includes: Chrysanthemum [45]; Ocimum sanctum and Zinnia elegans [34]; Amaranthus tricolor, Datura innoxia and Hyoscyamus muticus [44]; Gerbera [53]; Vanilla [28]; Pelargonium [52]; Piper longum and Piper betel [19]; Rouvolfia serpentine [35]; Jatropha curcas [36]; Catharanthus roseus [40]; Daucas carrota [1]; Banana [24,29]; Lycopersicum esculentum [15,25,47]; Gladiolus [13]. Although many CMV strains were isolated from India, but incomplete information is available at the genome level about these isolates.…”

Section: Introductionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India.

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Properties of a Cucumber Mosaic Virus Strain Naturally Infecting Chrysanthemum in India

Cited by 30 publications

References 0 publications

First report of association of Cucumber mosaic virus with blister and leaf distortion of castor bean (Ricinus communis) in India

First report of association of Cucumber mosaic virus with blister and leaf distortion of castor bean (Ricinus communis) in India

Occurrence of Cucumber mosaic virus on Potato and its Transmission to Muskmelon under Potato-Cucurbit Cropping Pattern Followed in Punjab, India

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

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