Properties of a major β-glucosidase-BGL1 from Aspergillus niger NII-08121 expressed differentially in response to carbon sources

Singhania, Reeta Rani; Sukumaran, Rajeev K.; Rajasree, Kuni Parambil; Mathew, A. G.; Gottumukkala, Lalithadevi; Pandey, Ashok

doi:10.1016/j.procbio.2011.04.006

Cited by 59 publications

(22 citation statements)

References 17 publications

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“…were able to produce b-glucosidase with different isoform profiles and a higher level of b-glucosidase than produced by Trichoderma viride on pre-treated sugar cane bagasse (Adsul et al, 2004). The presence of b-glucosidase isoforms in fungi depending on different SSF substrates has already been reported (Singhania et al, 2011). Xylanase (EC 3.2.1.8) hydrolyses b-1,4-xylan,the most abundant component of hemicellulose, into xylose.…”

Section: Discussionmentioning

confidence: 92%

Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Grujić

Dojnov

Potočnik

et al. 2015

International Biodeterioration & Biodegradation

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Section: Discussionmentioning

confidence: 92%

Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Grujić

Dojnov

Potočnik

et al. 2015

International Biodeterioration & Biodegradation

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“…The result of this study agrees with some earlier reports in which many commercial β -glucosidases have been reported to exhibit optimum activity at acidic pH regions. Singhania and Karnchanatat et al reported similar optimum pH of 5.0 for β -glucosidase from A. niger NII 08121 and Daldinia eschscholzii , respectively [36, 37]. β -glucosidases from various species of Penicillium have been reported to have optimum pH range of 4.0–6.0 [38–40].…”

Section: Resultsmentioning

confidence: 99%

Production and Characterization of Highly Thermostableβ-Glucosidase during the Biodegradation of Methyl Cellulose byFusarium oxysporum

Olajuyigbe

Nlekerem

Ogunyewo

2016

Biochemistry Research International

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Production of β-glucosidase from Fusarium oxysporum was investigated during degradation of some cellulosic substrates (Avicel, α-cellulose, carboxymethyl cellulose (CMC), and methylcellulose). Optimized production of β-glucosidase using the cellulosic substrate that supported highest yield of enzyme was examined over 192 h fermentation period and varied pH of 3.0–11.0. The β-glucosidase produced was characterized for its suitability for industrial application. Methyl cellulose supported the highest yield of β-glucosidase (177.5 U/mg) at pH 6.0 and 30°C at 96 h of fermentation with liberation of 2.121 μmol/mL glucose. The crude enzyme had optimum activity at pH 5.0 and 70°C. The enzyme was stable over broad pH range of 4.0–7.0 with relative residual activity above 60% after 180 min of incubation. β-glucosidase demonstrated high thermostability with 83% of its original activity retained at 70°C after 180 min of incubation. The activity of β-glucosidase was enhanced by Mn2+ and Fe2+ with relative activities of 167.67% and 205.56%, respectively, at 5 mM and 360% and 315%, respectively, at 10 mM. The properties shown by β-glucosidase suggest suitability of the enzyme for industrial applications in the improvement of hydrolysis of cellulosic compounds into fermentable sugars that can be used in energy generation and biofuel production.

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“…For instances, multiplicity of β-glucosidase has been demonstrated in T. reesei [170,171]. A. niger NII-08121/MTCC 7956 was found to express four β-glucosidase isoforms when grown on lactose or cellulose, whilst 2 isoforms were expressed on wheat bran or rice straw as carbon source [172]. Sonia et al isolated thirteen thermophilic and thermotolerant fungi from composting soil and found that twenty-eight β-glucosidase isoforms were expressed when corn cob was used as carbon source.…”

Section: Multiplicity Of β-Glucosidasementioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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Cellulose is the most abundant biomaterial in the biosphere and the major component of plant biomass.Cellulase is an enzymatic system required for conversion of renewable cellulose biomass into free sugar for subsequent use in different applications. Cellulase system mainly consists of three individual enzymes namely: endoglucanase, exoglucanase and β-glucosidases. β-Glucosidases are ubiquitous enzymes found in all living organisms with great biological significance. β-Glucosidases have also tremendous biotechnological applications such as biofuel production, beverage industry, food industry, cassava detoxification and oligosaccharides synthesis. Microbial β-glucosidases are preferred for industrial uses because of robust activity and novel properties exhibited by them. This review aims at describing the various biochemical methods used for screening and evaluating β-glucosidases activity from microbial sources. Subsequently, it generally highlights techniques used for purification of β-glucosidases. It then elaborates various biochemical and molecular properties of this valuable enzyme such as pH and temperature optima, glucose tolerance, substrate specificity, molecular weight, and multiplicity. Furthermore, it describes molecular cloning and expression of bacterial, fungal and metagenomic β-glucosidases. Finally, it highlights the potential biotechnological applications of β-glucosidases.

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Properties of a major β-glucosidase-BGL1 from Aspergillus niger NII-08121 expressed differentially in response to carbon sources

Cited by 59 publications

References 17 publications

Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Production and Characterization of Highly Thermostableβ-Glucosidase during the Biodegradation of Methyl Cellulose byFusarium oxysporum

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

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