Properties of a New Food Supplement Containing Actinia equina Extract

Lanza, Marika; Casili, Giovanna; Torre, Giovanna Loredana La; Giuffrida, Daniele; Rotondo, Archimede; Esposito, Emanuela; Ardizzone, Alessio; Rando, Rossana; Bartolomeo, Giovanni; Albergamo, Ambrogina; Vadalà, Rossella; Salvo, Andrea

doi:10.3390/antiox9100945

Cited by 11 publications

(7 citation statements)

References 56 publications

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“…Histological evaluation was performed as previously described by Lanza et al 60 . The vaginal tissues were fixed with 10% neutral formalin, embedded in paraffin, and sectioned at 7 µm.…”

Section: Methodsmentioning

confidence: 99%

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Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

Lanza

Scuderi²,

Capra³

et al. 2023

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Bacterial vaginosis (BV) is a common vaginal dysbiosis characterized by a malodorous discharge and irritation. The imbalance of the vaginal microbiota plays a key role in the development of BV. It has been demonstrated that Gardnerella vaginalis (GV), a facultative anaerobic bacillus, is involved in BV. Due to the rising number of antimicrobial-resistant species, recurrence of BV is becoming more frequent in women; thus, alternative treatments to antibiotics are needed. Natural substances have recently shown a great efficacy for the treatment of vaginal dysbiosis. Thus, this study aimed to investigate the beneficial effect of a product containing pea protein (PP), grape seed extract (GS) and lactic acid (LA) in an in vivo model of Gardnerella vaginalis-induced vaginosis by intravaginal administration of GV suspension (1 × 106 CFU/20 µL saline). Our results demonstrated that the product containing PP, GS and LA significantly reduced GV proliferation. More specifically, it significantly preserved tissue architecture and reduced neutrophil infiltration, inflammatory markers and sialidase activity when used both as a pre- or a post-treatment. Moreover, the product displayed strong bioadhesive properties. Therefore, our data suggested that the product containing PP, GS and LA could be used as alternative preventive or curative treatment for the management of BV.

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“…Histological evaluation was performed as previously described by Lanza et al 60 . The vaginal tissues were fixed with 10% neutral formalin, embedded in paraffin, and sectioned at 7 µm.…”

Section: Methodsmentioning

confidence: 99%

“…MPO activity, an index of polymorphonuclear cell accumulation, was determined as previously described by Lanza et al 60 . The rate of change in absorbance was measured spectrophotometrically at 650 nm.…”

Section: Methodsmentioning

confidence: 99%

Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

Lanza

Scuderi²,

Capra³

et al. 2023

Sci Rep

Self Cite

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“…MPO levels were assessed as previously described [ 35 ]. The change in absorbance was calculated via a spectrophotometer at 650 nm.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of the Radical Scavenger, Tempol, to Reduce Inflammation and Oxidative Stress in a Murine Model of Atopic Dermatitis

et al. 2023

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Atopic dermatitis (AD) is the most common chronically relapsing inflammatory skin disease, predominantly common in children; it is characterized by an eczematous pattern generally referable to skin dryness and itchy papules that become excoriated and lichenified in the more advanced stages of the disease. Although the pathophysiology of AD is not completely understood, numerous studies have demonstrated the complex interaction between genetic, immunological, and environmental factors, which acts to disrupt skin barrier function. Free radicals play a key role by directly damaging skin structure, inducing inflammation and weakening of the skin barrier. Tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) is a membrane-permeable radical scavenger, known to be a stable nitroxide, which exhibits excellent antioxidant effects in several human disorders, such as osteoarthritis and inflammatory bowel diseases. Considering the few existing studies on dermatological pathologies, this study aimed to evaluate tempol, in a cream formulation, in a murine model of AD. Dermatitis was induced in mice via dorsal skin application of 0.5% Oxazolone, three times a week for two weeks. After induction, mice were treated with tempol-based cream for another two weeks at three different doses of 0.5%, 1% and 2%. Our results demonstrated the ability of tempol, at the highest percentages, to counteract AD by reducing the histological damage, decreasing mast cell infiltration, and improving the skin barrier properties, by restoring the tight junction (TJs) and filaggrin. Moreover, tempol, at 1% and 2%, was able to modulate inflammation by reducing the nuclear factor kappa-light-chain-enhancer of the activated B cell (NF-κB) pathway, as well as tumor necrosis factor (TNF)-α and interleukin (IL)-1β expression. Topical treatment also attenuated oxidative stress by modulating nuclear factor erythroid 2-related factor 2 (Nrf2), manganese superoxide dismutase (MnSOD), and heme oxygenase I (HO-1) expression levels. The obtained results demonstrate the numerous advantages provided by the topical administration of a tempol-based cream formulation, in reducing inflammation and oxidative stress through modulation of the NF-κB/Nrf2 signaling pathways. Therefore, tempol could represent an alternative anti-atopic approach to treating AD, thereby improving skin barrier function.

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“…The ultrasound-assisted extraction was repeated with a fresh n-hexane/ethyl acetate mixture for three cycles; the upper organic phases were pooled and dried under nitrogen. The residue was dissolved in 1 mL of methyl-terz-butyl-ether (MTBE)/methanol (1:1, v/v), filtered through 0.45 µm PTFE, and analyzed by HPLC following a method we used for other carotenoid determinations [31,32].…”

Section: Carotenoid Extraction and Analysis By High-performance Liqui...mentioning

confidence: 99%

Profile of Carotenoids and Tocopherols for the Characterization of Lipophilic Antioxidants in “Ragusano” Cheese

et al. 2021

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Lipophilic antioxidants such as carotenoids and tocopherols are appreciated components in food because of their potential health benefits. The aim of this study was to describe the composition of these microconstituents in “Ragusano”, a typical Sicilian historical pasta filata cheese, and to compare them during two different production seasons. Specifically, the tocopherols’ composition was evaluated by high-performance liquid chromatography coupled to a fluorescence detector (HPLC-FD); whereas the contents of three main carotenoids were determined by high-performance liquid chromatography coupled to a diode array detector with atmospheric pressure chemical ionization and mass spectrometry (HPLC-DAD-APCI-MS). The scope included studying the influence of dietary supplementation on the potential enrichment of “Ragusano” in antioxidants. The main results regarding the composition of lipophilic vitamins of 56 “Ragusano” cheeses, collected in winter and spring, revealed that α-tocopherol was the predominant component amongst tocopherols and carotenoids, while β-carotene prevailed among the carotenoids. The cheeses obtained in spring turned out to contain larger amounts of antioxidants, both tocopherols and carotenoids, while the dietary supplementation with minerals-vitamins led to a barely detectable increase of antioxidants compared to a measured control group.

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Properties of a New Food Supplement Containing Actinia equina Extract

Cited by 11 publications

References 56 publications

Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

Effect of a combination of pea protein, grape seed extract and lactic acid in an in vivo model of bacterial vaginosis

Efficacy of the Radical Scavenger, Tempol, to Reduce Inflammation and Oxidative Stress in a Murine Model of Atopic Dermatitis

Profile of Carotenoids and Tocopherols for the Characterization of Lipophilic Antioxidants in “Ragusano” Cheese

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