Properties of a trichlorodibenzo-p-dioxin-dechlorinating mixed culture with a Dehalococcoides as putative dechlorinating species

Ballerstedt, Hendrik; Hantke, Janina; Bunge, Michael; Werner, Birgit; Gerritse, Jan; Andreesen, Jan R.; Lechner, Ute

doi:10.1016/s0168-6496(03)00282-4

Cited by 57 publications

(32 citation statements)

References 70 publications

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“…A TrCDD-dechlorinating river-sediment culture yielded "Dehalococcoides" sp. as a putative dehalorespiring species 12) . A "Dehalococcoides"-containing mixed culture reductively dechlorinated 1,2,4-TrCDD and 1,2,3-TrCDD, forming the main products 1,3-DCDD and 2-MCDD from the former congener and 2,3-DCDD from the latter, with carbon isotope fractionation taking place during the sequential reductive dechlorination 40) .…”

Section: Pcdd/f-dehalogenating Bacteria and Consortiamentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called dehalorespiration or halorespiration. Dehalorespiring bacteria may play important roles in the geochemical cycle with organohalogens in nature and have great promise in their application to the bioremediation of haloorganic contaminants derived from anthropogenic sources. During the past decade, a number of dehalorespiring microorganisms, including a unique group of strictly dehalorespiring bacteria, "Dehalococcoides", have been isolated and characterized at phylogenetic, physiologic, and genetic levels. Also, new perspectives of dehalorespiring bacteria have emerged based on information about genomics and molecular microbial ecology. This review article focuses on up-to-date knowledge of the biodiversity of dehalorespiring bacteria and reductively dehalogenating microbial consortia with special emphasis on those capable of transforming polychlorinated biphenyls and dioxins.

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Section: Pcdd/f-dehalogenating Bacteria and Consortiamentioning

confidence: 99%

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Hiraishi

2008

Microb. Environ.

103

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“…Probably, there exists a large group of dehalorespiring bacteria within the phylum Chloroflexi, including "Dehalococcoides" species and their phylogenetic relatives reported to date as uncultured dechlorinating bacteria 15,40,57,58,60) . This assembly of dehalo-respiring bacteria, termed here the "Dehalococcoides" group, may be omnipresent in a wide range of contaminated environments and contribute to the natural attenuation of PCDD/Fs and other chlorinated contaminants 6,15,22) . However, little is known about the potential capacity of natural microbial communities to transform PCDD/Fs and the relationship between this biological process and the population dynamics of the "Dehalococcoides" group of bacteria.…”

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confidence: 99%

Biotransformation of Polychlorinated Dioxins and Microbial Community Dynamics in Sediment Microcosms at Different Contamination Levels

et al. 2005

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Semi-anaerobic microcosms containing different levels of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed by seeding with different mass ratios of lake sediment and dioxin-contaminated soil and incubating with organic medium for 1 year. In all microcosms, PCDD/Fs were reduced as a first-order reaction with similar removal rate coefficients, and only trace amounts of less chlorinated congeners were produced as the intermediate and end products. This apparent complete dechlorination of PCDD/Fs seemed to be due to a combination of reductive dechlorination of PCDD/Fs and oxidative degradation of the dechlorinated products. Total cell counting, 16S rRNA gene clone library analyses and quinone profiling showed that the microcosms contained relatively constant total populations with members of the phyla Bacteroidetes, Firmicutes and Proteobacteria (especially "Deltaproteobacteria") as the major constituents, independent of pollution levels. Quantitative real-time PCR with a specific primer set showed that the population density of "Dehalococcoides" and its phylogenetic relatives was highly correlated with the concentration of PCDD/Fs present. Some "Dehalococcoides" strains were isolated from the microcosms by repeated enrichment with chloroaromatics as the terminal electron acceptor. However, these isolates did not match with the major "Dehalococcoides"-related clones directly PCR-amplified. The results of this study suggest that PCDD/Fs in natural environments under given conditions are transformed with similar half-reduction rates independent of their concentrations, and a wide variety of "Dehalococcoides"-related bacteria play the primary role in this process.

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“…The DNA was eluted in 50–100 μl water and served as template (1 μl) for polymerase chain reactions (PCR) using the HotStarTaq Polymerase (Qiagen, Hilden, Germany) according to the instructions of the manufacturer and 40 cycles of amplification. The following primers were used to detect Dehalococcoides : DET730f/DET1350r (Ballerstedt et al ., 2004), the latter with one degenerate position to match also members of the Pinellas group of Dehalocococides mccartyi ; Dehalobacter : DRE445f (Ballerstedt et al ., 2004)/DRE1248r (5′‐GGCTTCGCTTCCGTCTG‐3′); and Desulfitobacterium : DES436f (5′‐TGTCTTCAGGGACGAACG‐3′)/DES1027r (5′‐CTCATAGCTCCCCGAAGG‐3′). The latter three primers were designed using the ARB software (Ludwig et al ., 2004).…”

Section: Methodsmentioning

confidence: 99%

Desulfitobacterium contributes to the microbial transformation of 2,4,5‐T by methanogenic enrichment cultures from a Vietnamese active landfill

Lechner

Türkowsky

Dinh

et al. 2018

Microbial Biotechnology

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SummaryThe herbicide 2,4,5‐trichlorophenoxyacetic acid (2,4,5‐T) was a major component of Agent Orange, which was used as a defoliant in the Vietnam War. Little is known about its degradation under anoxic conditions. Established enrichment cultures using soil from an Agent Orange bioremediation plant in southern Vietnam with pyruvate as potential electron donor and carbon source were shown to degrade 2,4,5‐T via ether cleavage to 2,4,5‐trichlorophenol (2,4,5‐TCP), which was further dechlorinated to 3,4‐dichlorophenol. Pyruvate was initially fermented to hydrogen, acetate and propionate. Hydrogen was then used as the direct electron donor for ether cleavage of 2,4,5‐T and subsequent dechlorination of 2,4,5‐TCP. 16S rRNA gene amplicon sequencing indicated the presence of bacteria and archaea mainly belonging to the Firmicutes, Bacteroidetes, Spirochaetes, Chloroflexi and Euryarchaeota. Desulfitobacterium hafniense was identified as the dechlorinating bacterium. Metaproteomics of the enrichment culture indicated higher protein abundances of 60 protein groups in the presence of 2,4,5‐T. A reductive dehalogenase related to RdhA3 of D. hafniense showed the highest fold change, supporting its function in reductive dehalogenation of 2,4,5‐TCP. Despite an ether‐cleaving enzyme not being detected, the inhibition of ether cleavage but not of dechlorination, by 2‐bromoethane sulphonate, suggested that the two reactions are catalysed by different organisms.

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Properties of a trichlorodibenzo-p-dioxin-dechlorinating mixed culture with a Dehalococcoides as putative dechlorinating species

Cited by 57 publications

References 70 publications

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Biodiversity of Dehalorespiring Bacteria with Special Emphasis on Polychlorinated Biphenyl/Dioxin Dechlorinators

Biotransformation of Polychlorinated Dioxins and Microbial Community Dynamics in Sediment Microcosms at Different Contamination Levels

Desulfitobacterium contributes to the microbial transformation of 2,4,5‐T by methanogenic enrichment cultures from a Vietnamese active landfill

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