Properties of an sv40‐transformed african green monkey kidney (bsc‐1) cell line

Suárez, H. G.; Bourali, M.‐F.; Wicker, R.; Estrade, S.; Cassingéna, R.

doi:10.1002/ijc.2910090210

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…SV40 rescue b y U V S E -m e d i a t e d cell fusion was p e r f o r m e d as described in a previous report (22). Aliquots of the same m i x e d cell suspensions used for virus rescue e x p e r i m e n t s were seeded on eoverslips in L e i g h t o n tubes (105 cells/ tube) a n d i n c u b a t e d a t 37 ° C. T w e n t y -f o u r hours later, t h e coverslip cultures were e x a m i n e d for establishing t h e p r o p o r t i o n of h e t e r o k a r y o n s using as a m a r k e r t h e SV40 specific T-antigen.…”

Section: Rescue Of Infectious S V Do Virus or S V 40 D N Amentioning

confidence: 95%

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Suárez

Lavialle

Estrade

et al. 1978

Archives of Virology

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A somatic cell hybrid (Cl. 6d) was originated from the fusion of mouse 3T3-4E) and spontaneous yielder SV 40-transformed Chinese hamster (CHK/SVLP AG) cells. During the early stages of its history, the C1. 6d hybrid underwent a rapid chromosome loss, preferentially loosing hamster chromosomes. This was not a constant tendency of the hybrid cells. As the parental CHK)SVLP AG cells, the hybrid cells were always found 100 per cent SV40 T-antigen positive. While CHK/SVLP AG cells infectious SV 40 DNA, V-antigen and virus were regularly detected, in the hybrid cells only infectious DNA was occasionally detected. This was not due either to the loww of an essential Chinese hamster gene(s) or to the presence of an inhibiting mouse cell component(s); it was apparently the consequence of inability of the cells to properly activate the resident SV 40 genome(s). After superinfection with SV 40 DNA, the hybrid cells-though capable of synthesizing SV 40 V-antigen--were unable to ensure virus assembly. Experimental evidence was obtained suggesting that SV 40 maturation is dependent of a cellular function(s).

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Section: Rescue Of Infectious S V Do Virus or S V 40 D N Amentioning

confidence: 95%

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Suárez

Lavialle

Estrade

et al. 1978

Archives of Virology

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“…The large tumor antigen (T antigen) of simian virus 40 (SV40), the major regulatory protein product of the early region of the viral genome, functions in the viral life cycle to initiate DNA replication (83), enhance late region transcription (1,17,35,36,58,65), and down-regulate early region transcription (1,36,64,85). In addition to its role in regulating the program for propagation of the virus, T antigen exerts profound effects on cellular metabolism, including stimulation of cellular DNA synthesis (11,20,31,32,69,77,86), transcription of specific cellular genes (76)(77)(78), and in some cell lines morphological transformation (5,6,24,37,38,50,57,73,79,82,84).…”

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confidence: 99%

trans-dominant defective mutants of simian virus 40 T antigen

Farber¹,

Peden²,

Nathans³

1987

J Virol

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We constructed a collection of linker insertion mutants in the simian virus 40 (SV40) genome and studied several of these with changes limited to a part of the large T antigen gene corresponding to an amino acid sequence shared with other ATPases. Two of these mutants were found to have a novel phenotype in that they could not be complemented for plaque formation by a late-region deletion mutant. These two mutants, in contrast to other mutants in this region, were able to transform rat cells in culture at a frequency close to that of the wild-type gene. The noncomplementing mutants were found to be potent inhibitors of SV40 DNA replication despite the presence of wild-type T antigen in the transfected cells. This inhibition was shown to be the result of the introduced mutations in the large T antigen gene. We conclude that the large T antigens of the noncomplementing mutants can act as inhibitors of SV40 DNA replication.

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“…If virions become regularly detectable, this question will be studied by the infectious center technique (23).…”

Section: Assay For Activationmentioning

confidence: 99%

“…The interaction of animal cells with Simian Virus 40 (SV40) has been extensively studied (1,3,8,11,16,17,22,23,25). Infection by SV40 can be either productive (leading to virus replication and death of the host cell) or abortive (resulting in blockage of virus replication at an intermediate stage and survival of the host cell).…”

mentioning

confidence: 99%

Activation of the Viral Genome in Simian Virus 4-Transformed Nonpermissive Cells by Permissive Cell Extracts

Suárez

Sonenshein

Estrade

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

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Simian virus 40-transformed nonpermissive cells in which neither infectious viral DNA nor virions had previously been detected reproducibly yield low levels of infectious simian virus-40 DNA after treatment with extracts of normal permissive cells. Virions were obtained in one out of 37 experiments. Activation of the viral genome is detected only with normal permissive cell extracts and only in simian virus 40-transformed cell lines that are rescuable by heterokaryon formation or by treatment with chemicals. The activating factor(s) is insensitive to deoxyribonuclease and ribonuclease, but is sensitive to heat at 560 and to proteolytic enzymes.

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Properties of an sv40‐transformed african green monkey kidney (bsc‐1) cell line

Abstract: An SV40-transformed BSC-1 cell line (BSC-SV CI I J ) , that had been cloned

Cited by 12 publications

References 21 publications

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

Expression of ?early? and ?late? viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder SV 40-transformed Chinese hamster cell

trans-dominant defective mutants of simian virus 40 T antigen

Activation of the Viral Genome in Simian Virus 4-Transformed Nonpermissive Cells by Permissive Cell Extracts

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