Properties of an SV40‐transformed hamster cell line resistant to actinomycin D

Ganglioside patterns of a cloned Simian virus 40-(SV40) induced hamster tumor cell (CI2TSV 5-S), its normal variant (CI 2TSV 5-R) which are C12TSV 5-S gradually adapted to grow in the presence of 2 µg/ml actinomycin D and exhibit certain normal phenotypic characteristics, and its back variant (C12TSV 5-RR), which are C12TSV5 -R cells grown in the absence of actinomycin D for more than 60 passages and which exhibit greater phenotypic similarity to CI2TSV 5-S cells, have been analyzed . All three cell lines contain N(acetylneuraminyl) galactosylglycosyl ceramide (hematoside, GM3), N-acetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosyl ceramide (GM2), and a higher ganglioside tentatively identified as disialohematoside . However, C12TSV5-R have more GM2 than C12TSV5-S whereas C12TSV5-RR contain an intermediate amount of GM2 . The amount of GM2 is correlative with the activity of UDP-N-acetylgalactosamine : hematoside N-acetylgalactosaminyl transferase in the extract of the three cell lines and with their agglutination by wheat germ agglutinin .

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“…(28), with the actinomycin D-sensitive and -resistant hamster tumor cells, which appeared after our work had been submitted for publication .…”

Section: Characteristics Of the Cell Linesmentioning

confidence: 99%

“…The data described in Table I are consistent with similar observations made in a paper by Wicker et al . (28), with the actinomycin D-sensitive and -resistant hamster tumor cells, which appeared after our work had been submitted for publication .…”

Section: Characteristics Of the Cell Linesmentioning

confidence: 99%

Ganglioside Patterns and Phenotypic Characteristics in a Normal Variant and a Transformed Back Variant of a Simian Virus 40-Induced Hamster Tumor Cell Line

Nigam

Lallier

Brailovsky

1973

The Journal of Cell Biology

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Incorporation de l'antigène tumoral de transplantation spécifique du SV40 (TSTA‐SV40) dans le virus de la stomatite vésiculleuse cultivé en cellules de hamster transformées par le SV40

Ansel¹

1974

Intl Journal of Cancer

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INCORPORATION O F SV40-TUMOR SPECIFIC TRANSPLANTATION ANTIGEN (SV40-TSTA) IN VESICULAR STOMATITIS VIRUS GROWN IN SV40 TRANSFORMED HAMSTER CELLS Highly purified and inactivated VS V ( VSVl TS V-5 C1.2) grown in S V40 transjornzed hamster cells, TSV-5 C1.2, induces in hamster a transplantation immunity against host-tumor cells. Immunization with VSVlTSV-5 C1.2 gave more than a 10-fold protection against TS V-5 CI.2 tumor cells. The transplantation immunity confered by the immunization is specific for tumor cells which possess S V40-TSTA. V S V/ TS V-5 C1.2 protects hamsters against another SV40-transformed cell line, EHS Vi-C1.1. In contrast, immunization with VS VIEHB, a purified preparation of' VS V grown in spontaneously transjormed hamster cells, EHB, gave no protectionto TSV-5 C1.2 cell challenge. It was concluded that the presence of S V40-TSTA activity in V S V/ TS V-5 C1.2 virions was due to incorporation of this tumor cell antigen in the viral envelope. The existence of cell antigens in VSV/TSV-5 C1.2 and VSVIEHB preparations was also shown in vitro by Cr5I release assay. The anti-VSVITSV-5 CI.2 rabbit serum reacted specifically with S V40-transformed cells. TS V-5 CI .2 and EHS Vi-CI .I.

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Actinomycin‐D‐resistant in vitro mouse cell line derived from a methylcholanthrene‐induced sarcoma: Decrease of malignancy and antigenic characteristics

Belehradek

Biedler

Thonier

et al. 1974

Intl Journal of Cancer

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The tumorigenic EPO clonal cell line, derived from a methylcholanthrene-induced murine sarcoma, was exposed to increasing concentrations of actinomycin D and gave rise to a subline, resistant to 0.02 mug of actinomycin D per ml of medium, which was designated EPO/ADj. Drug resistance was accompained by a striking decrease of tumorigenic capacity as determined by the tumor take incidence and tumor growth rate in syngeneic C57BL/6 mice (normal and X-irradiated), as well as by tumor take incidence in the cheek pouch of cortisone-treated weanling Syrian hamsters. One of the tumors obtained after inoculation of EPO/ADj cells into syngeneic mice was reexplanted in culture and developed as the EPO/ADj/T subline. It was found to be relatively resistant to actinomycin D as compared to the parental EPO cells. The morphology of cells changed with actinomycin-D-resistance: EPO/ADj cells were more spread and flattened and less overlapping than the original fibroblast-like EPO cells. EPO/ADj/T cells, however, had an aspect similar to that of EPO cells. No major differences were seen between the karyotypes of EPO, EPO/ADj and EPO/ADj/T cells. Tumor-specific antigen(s), characteristic of drug-sensitive, parental EPO line, was expressed in the resistant EPO/ADj and EPO/ADj/T lines, as shown by: (a) transplantation resistance to challenge with EPO cells in syngeneic mice pretreated with EPO/ADj cells; and (b) cross-reactivity in indirect immunofluorescence tests performed with sera from syngeneic animals hyperimmunized with EPO or EPO/ADj cells. Furthermore, an additional surface antigen(s), absent from EPO cells, was demonstrated in EPO/ADj and EPO/ADj/T cells by means of immunofluorescence tests performed with specific anti-EPO/ADj syngeneic sera previously absorbed on EPO or EPO/ADj cells.

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Properties of an SV40‐transformed hamster cell line resistant to actinomycin D

Cited by 12 publications

References 10 publications

Ganglioside Patterns and Phenotypic Characteristics in a Normal Variant and a Transformed Back Variant of a Simian Virus 40-Induced Hamster Tumor Cell Line

Ganglioside Patterns and Phenotypic Characteristics in a Normal Variant and a Transformed Back Variant of a Simian Virus 40-Induced Hamster Tumor Cell Line

Incorporation de l'antigène tumoral de transplantation spécifique du SV40 (TSTA‐SV40) dans le virus de la stomatite vésiculleuse cultivé en cellules de hamster transformées par le SV40

Actinomycin‐D‐resistant in vitro mouse cell line derived from a methylcholanthrene‐induced sarcoma: Decrease of malignancy and antigenic characteristics

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