Properties of Anti-CA125 antibody layers on screen-printed carbon electrodes modified by gold and platinum nanostructures

Baradokė, Aušra; Jose, Bincy; Pauliukaitė, Rasa; Forster, Robert J.

doi:10.1016/j.electacta.2019.03.081

Cited by 31 publications

(23 citation statements)

References 29 publications

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“…Despite the improved detection performance of non-faradaic electrochemical immunosensors, it has been reported that changing one parameter of the antibody-electrode ('bioelectronic') interface can drastically affect the detection performance; therefore, this should be carefully studied. Although some electrochemical parameters of the bioelectronic interface, such as the electrode surface area [11], the electrode material [12], or the linker concentration [13], have already been reported to drastically affect the resulting sensitivity, only limited information has been reported on biological components, such as the antibody probe density and the antigen analyte incubation time.…”

Section: Introductionmentioning

confidence: 99%

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

et al. 2020

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Electrochemical sensors based on antibody-antigen recognition events are commonly used for the rapid, label-free, and sensitive detection of various analytes. However, various parameters at the bioelectronic interface, i.e., before and after the probe (such as an antibody) assembly onto the electrode, have a dominant influence on the underlying detection performance of analytes (such as an antigen). In this work, we thoroughly investigate the dependence of the bioelectronic interface characteristics on parameters that have not been investigated in depth: the antibody density on the electrode's surface and the antigen incubation time. For this important aim, we utilized the sensitive non-faradaic electrochemical impedance spectroscopy method. We showed that as the incubation time of the antigen-containing drop solution increased, a decrease was observed in both the solution resistance and the diffusional resistance with reflecting boundary elements, as well as the capacitive magnitude of a constant phase element, which decreased at a rate of 160 ± 30 kΩ/min, 800 ± 100 mΩ/min, and 520 ± 80 pF × s (α-1) /min, respectively. Using atomic force microscopy, we also showed that high antibody density led to thicker electrode coating than low antibody density, with rootmean-square roughness values of 2.2 ± 0.2 nm versus 1.28 ± 0.04 nm, respectively. Furthermore, we showed that as the antigen accumulated onto the electrode, the solution resistance increased for high antibody density and decreased for low antibody density. Finally, the antigen detection performance test yielded a better limit of detection for low antibody density than for high antibody density (0.26 μM vs 2.2 μM). Overall, we show here the importance of these two factors and how changing one parameter can drastically affect the desired outcome.

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Section: Introductionmentioning

confidence: 99%

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

et al. 2020

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“…The detection of the same CA 125 biomarker was recorded on another immunosensor based on SPEs modified with AuNPs (“popcorn” nanostructures with 566 nm) and PtNPs (140 nm). The influence of the two nanomaterials on the electrochemical parameters have been compared, and the PtNPs presented the highest surface area leading to a lower limit of detection [ 242 ]. Carcinoembryonic antigen was determined on an immunosensor based on a SPE modified in one-step preparation with GO (electrochemically reduced) and AuNPs (140 nm—deposited by the electrochemical method).…”

Section: Metal Nanoparticles and Carbon-based Nanomaterials In (Bio)sensors Designmentioning

confidence: 99%

Metal Nanoparticles and Carbon-Based Nanomaterials for Improved Performances of Electrochemical (Bio)Sensors with Biomedical Applications

et al. 2021

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Monitoring human health for early detection of disease conditions or health disorders is of major clinical importance for maintaining a healthy life. Sensors are small devices employed for qualitative and quantitative determination of various analytes by monitoring their properties using a certain transduction method. A “real-time” biosensor includes a biological recognition receptor (such as an antibody, enzyme, nucleic acid or whole cell) and a transducer to convert the biological binding event to a detectable signal, which is read out indicating both the presence and concentration of the analyte molecule. A wide range of specific analytes with biomedical significance at ultralow concentration can be sensitively detected. In nano(bio)sensors, nanoparticles (NPs) are incorporated into the (bio)sensor design by attachment to the suitably modified platforms. For this purpose, metal nanoparticles have many advantageous properties making them useful in the transducer component of the (bio)sensors. Gold, silver and platinum NPs have been the most popular ones, each form of these metallic NPs exhibiting special surface and interface features, which significantly improve the biocompatibility and transduction of the (bio)sensor compared to the same process in the absence of these NPs. This comprehensive review is focused on the main types of NPs used for electrochemical (bio)sensors design, especially screen-printed electrodes, with their specific medical application due to their improved analytical performances and miniaturized form. Other advantages such as supporting real-time decision and rapid manipulation are pointed out. A special attention is paid to carbon-based nanomaterials (especially carbon nanotubes and graphene), used by themselves or decorated with metal nanoparticles, with excellent features such as high surface area, excellent conductivity, effective catalytic properties and biocompatibility, which confer to these hybrid nanocomposites a wide biomedical applicability.

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“…Recently, Baradoke et al [26] constructed Screen-printed carbon electrodes were electroplated with gold or platinum nanostructures and used as an antibody immobilization platform for CA125. In this works, nanostructured surfaces have been used as a tool for the design of immunosensors and analyses in electrochemical kinetics such as antibody immobilization, electrode surface blocking and antigen binding.…”

Section: Cancer Antigen 125 (Ca125)mentioning

confidence: 99%

Current and Prospective of Breast Cancer Biomarkers

Benjamin¹,

Lima²

2021

Molecular Biotechnology

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Biomarkers have shown great promise over the past decade the process of drug development more effective and have become an integral part of diagnosis of diseases. Biosensors were integrated with biomarker detection and point-of-care detection for signal amplification, high specificity and sensitivity, rapid response time, low cost, simplicity and multi-analytical testing. In order to detect more sensitively, these particular biomarkers have been explored with the possibility of real-time measurements in order to develop simple and compact systems which can analyze complex specimens. Various biosensors including electrochemical biosensors have recently been developed based on disease-specific biomarkers in the diagnosis of cancer disease. The main objective of the book chapter is to review research with new materials/methods in electrochemical biosensing techniques to detection of breast cancer biomarkers and evaluating latest techniques for detection of important analytes in real samples. In this book chapter, the recent development of electrochemical biosensors of breast cancer biomarkers will be reviewed. Furthermore, recent and future trend application of breast cancer biomarkers will be discussed.

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Properties of Anti-CA125 antibody layers on screen-printed carbon electrodes modified by gold and platinum nanostructures

Cited by 31 publications

References 29 publications

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

Probing antibody surface density and analyte antigen incubation time as dominant parameters influencing the antibody-antigen recognition events of a non-faradaic and diffusion-restricted electrochemical immunosensor

Metal Nanoparticles and Carbon-Based Nanomaterials for Improved Performances of Electrochemical (Bio)Sensors with Biomedical Applications

Current and Prospective of Breast Cancer Biomarkers

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