Properties of bacterial cellulose produced in grape medium by native isolate Gluconacetobacter sp

Abstract

During the production of grape wine, the occurrence of thick leathery pellicle at the air‐liquid interface was found as a contaminant. The pellicle produced was investigated with a view to use as biodegradable polymer. The bacterium that is responsible for the pellicle production was isolated, characterized and identified as Gluconacetobacter sp. Pellicle was produced in pasteurized grape extract as well as in HS medium by the isolated organism in static conditions. The purified film was subjected for Fourier transform infrared spectroscopy and C13 solid NMR spectroscopy analysis, which confirmed the pellicle to be a cellulosic material. Scanning Electron Micrograph showed ultra fine network structure along with cells. The films were tested for its physicomechanical characters, barrier and thermal properties. The films of 25‐μ thickness showed very high tensile strength (41.158 MPa) and elongation of 0.987 mm. The thermal properties of the films were characterized by Differential scanning calorimetry and Thermo gravimetric analysis. The melt peak temperature was found to be 111.65°C. The percentage of weight loss was found to be 20% at 327.86°C. Barrier properties (oxygen transmission rate and water vapor transmission rate), indicated a high oxygen barrier but low water barrier. This is the first report on the barrier properties of bacterial cellulose from Gluconacetobacter sp. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Isolation and identification of cellulose-producing strain Komagataeibacter intermedius from fermented fruit juice

“…were isolated from kombucha tea, one of fermented beverages. Rani, Udayasankar, and Appaiah (2011) found that the pellicle formed in contaminated grape wine. The bacterium forming the pellicle production was isolated, characterized and identified as Gluconacetobacter sp.…”

Bacterial Cellulose Fibers

“…Films of 25 μm thickness were found to have an unusually high tensile strength of 41 MPa and elongation of 0.98 mm. The films had low oxygen permeability but high water permeability [11Ran]. Figure 61.10 shows an SEM image of the bacterial cellulose generated by the cells.…”

“…Morphologically, the fibers had a rough surface with pores in the middle that were responsible for the poor tensile strength. Bacterial cellulose had unique properties such as high porosity, high purity and crystallinity, good mechanical properties and high water holding capacity, excellent biodegradability, and biocompatibility [12Wan] which make it [11Ran] preferable for applications in batteries, sensors, electrical devices, and antistatic coating. To utilize these advantages, bacterial cellulose in nanofiber form was blended with poly(aniline) and made into composites with flake shaped morphology with high electrical conductivity.…”

Isolation and Identification of a Cellulose-Producing Bacterial Strain from the Genus Bacillus

“…[5][6][7][8]. At present, bacterial strains such as Agrobacterium, Rhizobium, Acetobacter, Aerobacter, Gluconacetobacter have been reported for BC production under optimum bioprocess conditions [9][10][11][12]. Acetobacter xylinum is one of the most extensively studied sources of BC [13].…”