1980
DOI: 10.1128/jb.142.2.433-438.1980
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Properties of beta-galactosidase III: implications for entry of galactosides into Klebsiella

Abstract: Klebsiella sp. strain CT-200 lacks both its plasmid-borne lac operon, which specifies ,-galactosidase I, and its chromosomal lac operon, which specifies flgalactosidase II, but it expresses a gene for a third,8-galactosidase, ,B-galactosidase Ill, constitutively. CT-200 was examined to determine whether there was a,galactoside permease associated with the ,B-galactosidase III gene. The failure of CT-200 to transport thiomethyl-fl-galactoside, o-nitrophenyl-fl-D-galactopyranoside, phenyl-,B-galactoside, lactulo… Show more

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Cited by 4 publications
(5 citation statements)
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“…BGase-III is inducible by y?-methyl-galactoside in strain RE1755A (Hall, 1979), and it hydrolyzes phenyl-/?-galactoside rapidly enough to permit growth (Hall, 1980). Strain RE1755A was mutagenized, and the mutagenized culture was grown in the presence of /?-methyl-galactoside to induce BGase-III synthesis.…”
Section: (Ii) Isolation Of a Temperature Sensitive Ptsl Mutantmentioning
confidence: 99%
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“…BGase-III is inducible by y?-methyl-galactoside in strain RE1755A (Hall, 1979), and it hydrolyzes phenyl-/?-galactoside rapidly enough to permit growth (Hall, 1980). Strain RE1755A was mutagenized, and the mutagenized culture was grown in the presence of /?-methyl-galactoside to induce BGase-III synthesis.…”
Section: (Ii) Isolation Of a Temperature Sensitive Ptsl Mutantmentioning
confidence: 99%
“…Strain RE 1755 does synthesize an inducible /?-galactosidase designated /?galactosidase-III (BGase-III) (Hall & Reeve, 1977). Synthesis of /?-galactosidase-III is inducible by lactose and by /?-methyl-galactoside (Hall, 1979), but strain RE 1755 is unable to utilize lactose because (i) BGase-III has very low activity toward lactose, and (ii) strain RE1755 possesses no /?-galactoside permease (Hall, 1980). Strain RE1755A is a streptomycin resistant nalidixic acid resistant mutant of strain RE1755 (Hall, 1979).…”
Section: Introductionmentioning
confidence: 99%
“…To elucidate the novel metabolic pathway for lactose metabolism involving P-BGase and to understand the pleiotropic mutation, we investigated lactose transport in Klebsiella strains. A previous report from this laboratory has described the absence of a,-galactoside permease as part of the BGase-III operon (5). Since (i) strain CT-1 grows on lactose and (ii) lactose induced P-BGase synthesis in both strains RE1755A and CT-1 (4), both strains must possess a lactose-specific transport system not associated with the BGase-III operon.…”
mentioning
confidence: 93%
“…(0.0015) (5). This is expected if strain CT-1 utilizes both hexose sugars released by hydrolysis of lactose.…”
mentioning
confidence: 94%
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