Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Arpornmaeklong, Premjit; Pripatnanont, Prisana; Suwatwirote, Narisorn

doi:10.1016/j.ijom.2007.11.014

Cited by 96 publications

(90 citation statements)

References 43 publications

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“…This indicates that 50Chit/50Col composition provides maximum stiffness to scaffold struts probably by the higher degree of formation of a complex between Col and Chit. The increase of compressive modulus of collagen-chitosan composite scaffolds was reported in the literature where better material stabilisation in terms of compression was achieved at a ratio of 1:2 chitosan-collagen (Arpornmaeklong, Pripatnanont, & Suwatwirote, 2008;Chicatun et al, 2011).…”

Section: Mechanicsmentioning

confidence: 55%

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Martinez

Blanco

Davidenko

et al. 2015

Carbohydrate Polymers

123

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Section: Mechanicsmentioning

confidence: 55%

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Martinez

Blanco

Davidenko

et al. 2015

Carbohydrate Polymers

123

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“…40,41 Many researchers have attempted to measure the swelling ratio of materials using gravimetric method. 42,43 However, the method may give us inaccurate information since loss of solution such as dropping or absorption ability of blot paper during process. Therefore, in order to make up for weak point of gravimetric method, volumetric method which measures the amount of remaining volume of water (PBS in this study) in the system, was used supplementally.…”

Section: Resultsmentioning

confidence: 99%

Morphological property and in vitro enzymatic degradation of modified chitosan as a scaffold

et al. 2011

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Chitosan (CS) was proposed as a promising candidate scaffold for tissue engineering. However, some drawbacks of natural CS remain. The current study modified CS by conjugating thiol to CS polymer (Thio-CS) and substantiated its three-dimensional microstructure and physical properties such as swelling or degradation. The Thio-CS was obtained by CS modification using 2-iminothiolane-HCl (2-IT). Because of the formation of disulfide bonds between thiol moieties based on oxidation of the immobilized thiol groups of CS, Thio-CS exhibits in situ gelling properties according to the reducing amount of free thiol. The content of the thiol group was increased as the amount of 2-IT increased. The swelling test demonstrated that Thio-CS can absorb up to 3.5 times its weight of phosphate buffered saline within 1 h and that the pore size and amount significantly increased with incubation time. The Thio-CS enzymatic degradation rate according to velocity was investigated. The results showed that Thio-CS was more resistant to lysozyme as viscosity increased. Thio-CS sponges were fabricated using freezedrying. The lyophilized Thio-CS had a homogeneous honeycomb-like shape, and its pores were relatively smaller (<2 µm) than those of unmodified CS (>2 µm). These results suggest that Thio-CS might be a candidate regenerative therapeutic device.

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“…[8][9][10][11] To investigate the osteogenicity of these potential bone graft materials, osteoprogenitor cells are cultured in vitro on scaffolds generated from the graft material and are analyzed for their effect on the osteogenic differentiation and proliferation of the seeded osteoprogenitor cells. [12][13][14][15] Research in our laboratory has previously demonstrated that a titanium mesh and extracellular matrix (ECM) composite scaffold was conducive to the osteogenic differentiation and proliferation of mesenchymal stem cells (MSCs), an osteoprogenitor cell population. 16,17 In the bone marrow cavity, osteoprogenitor cells are in contact with other bone marrow cell populations, mineralized ECM, and biological factors.…”

mentioning

confidence: 99%

Osteogenic Differentiation of Mesenchymal Stem Cells on Pregenerated Extracellular Matrix Scaffolds in the Absence of Osteogenic Cell Culture Supplements

Thibault

Baggett

Mikos

et al. 2010

Tissue Engineering Part A

186

177

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This study utilized a full-factorial design to investigate the effect of four factors: presence of whole bone marrow cells, presence of in vitro-generated mineralized extracellular matrix (ECM), presence of dexamethasone, and variations in culture duration, on the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) cultured on a polymer scaffold. Electrospun poly(e-caprolactone) (PCL) fiber mesh scaffolds were seeded with rat MSCs and cultured in complete osteogenic medium for 12 days to generate constructs containing mineralized ECM. MSCs or MSCs and whole bone marrow cells were seeded onto decellularized ECM constructs (PCL=ECM) or plain PCL scaffolds and cultured statically for 4, 8, and 16 days in medium either with or without dexamethasone. After each culture period, the cell number was determined by DNA analysis, and the osteogenic differentiation state of the cells was determined by alkaline phosphatase activity and calcium assays. MSCs seeded onto PCL=ECM constructs and cultured in medium either with or without dexamethasone demonstrated similar amounts of calcium deposition after 16 days. A significant increase in cell number over time compared with all other groups was observed when whole bone marrow cells were cocultured with MSCs on PCL scaffolds in medium without dexamethasone. This study establishes that the osteogenic differentiation of MSCs seeded onto ECM-containing constructs is maintained even in the absence of dexamethasone and that the coculture of MSCs and whole bone marrow cells without dexamethasone and ECM enhances the proliferation of a cell population (or populations) present in the whole bone marrow.

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Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Cited by 96 publications

References 43 publications

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties

Morphological property and in vitro enzymatic degradation of modified chitosan as a scaffold

Osteogenic Differentiation of Mesenchymal Stem Cells on Pregenerated Extracellular Matrix Scaffolds in the Absence of Osteogenic Cell Culture Supplements

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