Properties of density gradient-fractionated peripheral blood leukocytes from cattle infected with bovine leukemia virus

Kenyon, S.J.; Piper, Charles E.

doi:10.1128/iai.16.3.898-903.1977

Cited by 40 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spontaneous proliferation of PBMC from persistently lymphocytotic BLV-infected cows is IL-2 and viral antigen dependent. PBMC from persistently lymphocytotic cows spontaneously incorporate [ 3 H]thymidine when placed in culture, predominantly due to proliferation of B lymphocytes (27,28). In 14 individual experiments, spontaneous [ 3 H]thymidine uptake of PBMC from persistently lymphocytotic cows was 2.5 to 50 times that of uninfected cows measured in the same assay (combined data presented in Fig.…”

Section: Resultsmentioning

confidence: 85%

B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2

Trueblood

Brown

Palmer

et al. 1998

J Virol

View full text Add to dashboard Cite

Bovine leukemia virus (BLV)-induced persistent lymphocytosis is characterized by a polyclonal expansion of CD5+ B lymphocytes. To examine the role of the cytokine microenvironment in this virus-induced B-lymphocyte expansion, the expression of interleukin-2 (IL-2), IL-4, IL-10, and gamma interferon (IFN-γ) mRNA, was measured in stimulated peripheral blood mononuclear cells from persistently lymphocytotic BLV-infected cows, nonlymphocytotic BLV-infected cows, and uninfected cows. IL-2 and IL-10 mRNA expression and IL-2 functional activity were significantly increased when peripheral blood mononuclear cells from persistently lymphocytotic cows were stimulated with concanavalin A (ConA). Additionally, during persistent lymphocytosis, peak IL-2 and IL-10 mRNA expression was delayed, and elevated expression was prolonged. To determine the potential biologic importance of increased IL-2 and IL-10 expression, the response of isolated B lymphocytes from persistently lymphocytotic cows to human recombinant cytokines and to cytokine-containing supernatants from isolated T lymphocytes was examined. While recombinant human IL-10 (rhIL-10) did not consistently induce detectable changes, rhIL-2 increased viral protein (p24) and IL-2 receptor expression in isolated B lymphocytes from persistently lymphocytotic cows. Additionally, rhIL-2 and supernatant from ConA-stimulated T lymphocytes enhanced B-lymphocyte proliferation. The stimulatory activity of the T-lymphocyte supernatant could be completely inhibited with a polyclonal anti-rhIL-2 antibody. Finally, polyclonal anti-rhIL-2 antibody, as well as anti-BLV antibody, inhibited spontaneous proliferation of peripheral blood mononuclear cells from persistently lymphocytotic cows, demonstrating that the spontaneous lymphoproliferation characteristic of BLV-induced persistent lymphocytosis is IL-2 dependent and antigen dependent. Collectively, these findings strongly suggest that increased T-lymphocyte expression of IL-2 in BLV-infected cows contributes to development and/or maintenance of persistent B lymphocytosis.

show abstract

Section: Resultsmentioning

confidence: 85%

B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2

Trueblood

Brown

Palmer

et al. 1998

J Virol

View full text Add to dashboard Cite

show abstract

“…According to some reports, since surface immunoglobulin-positive (SIg') cells increase in EBL cattle, these tumour cells are of the B-cell lineage (1,6,17,18). O n the contrary, another report showed that peripheral blood lymphocytes (PBL) of cattle with EBL showed a decrease in SIg' cells (10).…”

Section: Introductionmentioning

confidence: 99%

Frequency of Lymphocytes bearing surface Membrane Immunoglobulins in Leukaemic Cattle

Koyama¹,

T²,

Yoshikawa³

et al. 1987

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

Summary The percentages of surface immunoglobuline‐positive (SIg+) cells in peripheral blood lymphocyte (PBL) and lymph nodes of 20 normal cattle were compared with those in the PBL, tumourous lymph nodes and tumour tissues of 33 cases of enzootic bovine leukosis (EBL). EBL animals were classified into three groups: one showing high SIg+ cell percentages in the lymphoid organs (SIgH group), one with low percentages (SIgL group) and one with intermediate values (SIgM group). Most of the EBL animals belonged to either the SIgH or the SIgL group. The number of such animals was 13:13 for PBL, 12:14 for the tumourous lymph nodes and 14:19 for tumour tissues. The number of SIgM animals was very small. Most of the animals with SIgH or SIgL tumour tissue were classified into the same SIg group for the lymphoid organs. The SIg+ cell percentages in all the specimens were low in the SIgL group: 13.2 ± 9.1% and 6.5 ± 6.0%, respectively, in the tumour tissue, as determined by antibovine Ig and IgG sera. Of the 31 EBL animals, 21 exhibited persistent lymphocytosis. Nine of these 21 were in the SIgH group, and 11, in the SIgL group, with no special bias found. These results indicate that the tumours were composed of surface immunoglobulin‐negative cells in some EBL animals. Zusammenfassung Häufigkeit von Lymphocyten mit Oberflächenmembran‐Immunglobulinen bei leukämischen Rindern Der prozentuale Anteil Oberflächen‐Immunglobulin positiver (SIg+) Zellen an peripheren Blutlymphocyten (PBL) und Lymphknoten von 20 normalen Rindern wurde mit dem an PBL, tumorösen Lymphknoten und Tumorgewebe von 33 Fällen enzootischer boviner Leukose (EBL) verglichen. Die EBL‐Tiere wurden in drei Gruppen eingeteilt: eine mit hoher Prozentzahl SIg+‐Zellen in den lymphoiden Organen (SIgH‐Gruppe), eine mit niedriger Prozentzahl (SIgL‐Gruppe) und eine mit mittleren Werten (SIgM‐Gruppe). Die meisten der EBL‐Tiere gehörten entweder zur SIgH‐ oder zur SIgL‐Gruppe. Die Anzahl dieser Tiere betrug 13:13 für PBL, 12:14 für tumoröse Lymphknoten und 14:19 für Tumorgewebe. Die Anzahl von SIgM‐Tieren war sehr gering. Die meisten Tiere mit SIgH‐ oder SIgL‐Tumorgewebe gehörten bezüglich lymphoider Organe in die gleiche SIg‐Gruppe. Der prozentuale Anteil von SIg+‐Zellen in Tumorgeweben aller Tiere der SIgL‐Gruppe, der mittels Antiseren gegen bovines Ig und IgG bestimmt wurde, betrug nur 13,2 ± 9,1% bzw. 6,5 ± 6,0%. Von den 33 EBL‐Tieren wiesen 21 eine persistierende Lymphocytose auf. Von diesen 21 Tieren gehörten 9 zur SIgH‐ und 11 zur SIgL‐Gruppe. Diese Ergebnisse weisen darauf hin, daß die Tumoren bei einigen EBL‐Tieren aus Oberflächen‐Immunglobulin negativen Zellen gebildet wurden.

show abstract

“…1985). In contrast to the HTLVs, however, B-cells are the principal target of BLV infection (Kenyon & Piper 1977b).…”

Section: Introductionmentioning

confidence: 99%

Evidence for BoLA‐linked resistance and susceptibility to subclinical progression of bovine leukaemia virus infection

Lewin

Bernoco

1986

Animal Genetics

View full text Add to dashboard Cite

The role of the bovine major histocompatibility complex in bovine leukaemia virus (BLV) infection and disease progression was investigated in a herd of Shorthorn cattle (n = 117). The frequency of cows that were seropositive to BLV-glycoprotein antigen was 51%. Twenty-three per cent of the seropositive cows were lymphocytotic. At the herd level, relative resistance to BLV-dependent B-cell proliferation and lymphocytosis among seropositive cows was associated with bovine lymphocyte antigen (BoLA)-DA7, whereas susceptibility was associated with BoLA-DA12.3. These associations were also confirmed at the family level, where BoLA phenotypes were used as haplotypic markers. Among the offspring of one BoLA-heterozygous sire (n = 33), resistance segregated with the DA7 haplotype and susceptibility with the DA12.3 haplotype. In this sire group, maternal transmission of the BoLA-w8 allele was associated with increased susceptibility to B-cell proliferation and lymphocytosis in w8/DA12.3 heterozygotes. These data provide the first evidence that subclinical progression of BLV infection is under the control of the BoLA complex, and suggest that the BoLA system can be used to select for resistance to B-cell proliferation and the development of lymphocytosis in BLV-infected herds.

show abstract

Properties of density gradient-fractionated peripheral blood leukocytes from cattle infected with bovine leukemia virus

Cited by 40 publications

References 6 publications

B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2

B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2

Frequency of Lymphocytes bearing surface Membrane Immunoglobulins in Leukaemic Cattle

Evidence for BoLA‐linked resistance and susceptibility to subclinical progression of bovine leukaemia virus infection

Contact Info

Product

Resources

About