Properties of DNA

Pedersen, Ronnie O.; Marchi, Alexandria N.; Majikes, Jacob M.; Nash, Jessica A.; Estrich, Nicole A.; Courson, David S.; Hall, Carol K.; Craig, Stephen L.; LaBean, Thomas H.

doi:10.1007/978-3-642-31107-9_10

Cited by 7 publications

(9 citation statements)

References 126 publications

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“…Deoxyribonucleic acid (DNA) has a broad range of physical, chemical, and biological properties making this biomolecule highly suitable for biosensor technologies. Among the most critical properties of DNA for a biosensor is its flexibility, easy synthesis, facile chemistry to attach to diverse platforms, simple regeneration and high specificity due to unique sequences of nucleotides [ 55 , 56 ]. However, several advantages and disadvantages of DNA biosensors have been identified.…”

Section: Graphene-based Nanomaterials and Deoxyribonucleic Acid (Dna)mentioning

confidence: 99%

Recent advances in graphene-based biosensor technology with applications in life sciences

et al. 2018

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Graphene’s unique physical structure, as well as its chemical and electrical properties, make it ideal for use in sensor technologies. In the past years, novel sensing platforms have been proposed with pristine and modified graphene with nanoparticles and polymers. Several of these platforms were used to immobilize biomolecules, such as antibodies, DNA, and enzymes to create highly sensitive and selective biosensors. Strategies to attach these biomolecules onto the surface of graphene have been employed based on its chemical composition. These methods include covalent bonding, such as the coupling of the biomolecules via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide reactions, and physisorption. In the literature, several detection methods are employed; however, the most common is electrochemical. The main reason for researchers to use this detection approach is because this method is simple, rapid and presents good sensitivity. These biosensors can be particularly useful in life sciences and medicine since in clinical practice, biosensors with high sensitivity and specificity can significantly enhance patient care, early diagnosis of diseases and pathogen detection. In this review, we will present the research conducted with antibodies, DNA molecules and, enzymes to develop biosensors that use graphene and its derivatives as scaffolds to produce effective biosensors able to detect and identify a variety of diseases, pathogens, and biomolecules linked to diseases.

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Section: Graphene-based Nanomaterials and Deoxyribonucleic Acid (Dna)mentioning

confidence: 99%

Recent advances in graphene-based biosensor technology with applications in life sciences

et al. 2018

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“…Over several decades, scientists have studied and discovered geometries of double-stranded DNA and RNA (Figure 1A). 14 Biomolecular engineers have made use of structural knowledge gleaned from nature as well as leveraging nucleic acids' propensity for programmed assembly by hybridization and self-folding to create a wide spectrum of useful artificial molecules and supramolecular assemblies. In this section, we will describe advances in both DNA and RNA structural engineering that have led to the array of functional assemblies described in later sections.…”

Section: Molecular Self-assembly Of Nucleic Acid Nanostructuresmentioning

confidence: 99%

“…Biological DNA structure is relatively simple and dominated by the rules of Watson–Crick base-pairing, while RNA displays more diverse and complex structural motifs in its larger biological assemblies such as the ribosome. Over several decades, scientists have studied and discovered geometries of double-stranded DNA and RNA (Figure A) . Biomolecular engineers have made use of structural knowledge gleaned from nature as well as leveraging nucleic acids’ propensity for programmed assembly by hybridization and self-folding to create a wide spectrum of useful artificial molecules and supramolecular assemblies.…”

Section: Molecular Self-assembly Of Nucleic Acid Nanostructuresmentioning

confidence: 99%

“…(A) Geometry of nucleic acid double helices: (left) B-form helix, normal geometry expected for dsDNA under physiologic-like solution conditions, and (right) A-form helix, normal geometry of dsRNA and special cases of dsDNA. Adapted with permission from ref . Copyright 2014 Springer-Verlag Berlin Heidelberg.…”

Section: Molecular Self-assembly Of Nucleic Acid Nanostructuresmentioning

confidence: 99%

“…(B) Four-arm (Holliday) junction schematics in 2D and 3D showing relative rotation of double-helices and double crossover (DX) complex shown in 2D and 3D schematics demonstrating rigid, coplanar ordering of paired double-helices. Adapted with permission from ref . Copyright 2014 Springer-Verlag Berlin Heidelberg.…”

Section: Molecular Self-assembly Of Nucleic Acid Nanostructuresmentioning

confidence: 99%

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Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

et al. 2021

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Researchers have worked for many decades to master the rules of biomolecular design that would allow artificial biopolymer complexes to self-assemble and function similarly to the diverse biochemical constructs displayed in natural biological systems. The rules of nucleic acid assembly (dominated by Watson–Crick base-pairing) have been less difficult to understand and manipulate than the more complicated rules of protein folding. Therefore, nucleic acid nanotechnology has advanced more quickly than de novo protein design, and recent years have seen amazing progress in DNA and RNA design. By combining structural motifs with aptamers that act as affinity handles and add powerful molecular recognition capabilities, nucleic acid-based self-assemblies represent a diverse toolbox for use by bioengineers to create molecules with potentially revolutionary biological activities. In this review, we focus on the development of self-assembling nucleic acid nanostructures that are functionalized with nucleic acid aptamers and their great potential in wide ranging application areas.

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pH-Driven Actuation of DNA Origami via Parallel I-Motif Sequences in Solution and on Surfaces

2017

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As bottom up DNA nanofabrication creates increasingly complex and dynamic mechanisms, the implementation of actuators within the DNA nanotechnology toolkit has grown increasingly important. One such actuator, the I-motif, is fairly simple in that it consists solely of standard DNA sequences and does not require any modification chemistry or special purification beyond that typical for DNA oligomer synthesis. This study presents a new implementation of parallel I-motif actuators, emphasizing their future potential as drivers of complex internal motion between substructures. Here we characterize internal motion between DNA origami substructures via AFM and image analysis. Such parallel I-motif design and quantification of actuation provide a useful step toward more complex and effective molecular machines.

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Properties of DNA

Cited by 7 publications

References 126 publications

Recent advances in graphene-based biosensor technology with applications in life sciences

Recent advances in graphene-based biosensor technology with applications in life sciences

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

pH-Driven Actuation of DNA Origami via Parallel I-Motif Sequences in Solution and on Surfaces

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