Properties of electrode-supported lipid cubic mesophase films with embedded gramicidin A: structure and ion-transport studies

Buta, A.; Nazaruk, Ewa; Dziubak, Damian; Szewczyk, Adam; Bilewicz, Renata

doi:10.1016/j.bioelechem.2021.108042

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…Research into X-ray scattering and circular dichroism revealed that the parameters of the lipid mesophase can change upon peptide addition and that the peptide conformation is dependent on LCP characteristics such as hydrophobic mismatch, the putative lateral pressure profile, and the intrinsic surface curvature of each lipid system. Properties of electrode-supported lipid cubic mesophase films with embedded gramicidin were recently studied [153]. LCPs with embedded proteins can reveal fundamental biological processes and protein-lipid interactions [135,150,154].…”

Section: Lipid Cubic Mesophases As Membranes For Embedding Potassium ...mentioning

confidence: 99%

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

Walewska

Krajewska

Stefanowska

et al. 2022

IJMS

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In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we focus on the critical evaluation of techniques used in the studies of mitochondrial potassium channels, describing their advantages and limitations. Functional analysis of mitochondrial potassium channels in comparison to that of plasmalemmal channels presents additional experimental challenges. The reliability of functional studies of mitochondrial potassium channels is often affected by the need to isolate mitochondria and by functional properties of mitochondria such as respiration, metabolic activity, swelling capacity, or high electrical potential. Three types of techniques are critically evaluated: electrophysiological techniques, potassium flux measurements, and biochemical techniques related to potassium flux measurements. Finally, new possible approaches to the study of the function of mitochondrial potassium channels are presented. We hope that this review will assist researchers in selecting reliable methods for studying, e.g., the effects of drugs on mitochondrial potassium channel function. Additionally, this review should aid in the critical evaluation of the results reported in various articles on mitochondrial potassium channels.

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Section: Lipid Cubic Mesophases As Membranes For Embedding Potassium ...mentioning

confidence: 99%

Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment

Walewska

Krajewska

Stefanowska

et al. 2022

IJMS

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“…Several successful electrochemical systems have been recently reported in which LCP is used to encapsulate various types of enzymes including hydrophilic 21 and membrane bound. 22,23 However, the effective development of LCP-entrapped enzyme electrodes requires the use of a redox shuttle molecule that facilitates the electron transfer between the enzyme and the electrode. With this purpose, water-soluble redox shuttles have been so far employed in the electrolyte, such as hydroquinone, 25 hexamine ruthenium, 26 and ferrocene carboxylic acid.…”

Section: ■ Introductionmentioning

confidence: 99%

“…LCP therefore represents an attractive platform for the development of enzymatic biosensing technology. Several successful electrochemical systems have been recently reported in which LCP is used to encapsulate various types of enzymes including hydrophilic and membrane bound. , …”

Section: Introductionmentioning

confidence: 99%

Development of Redox-Active Lyotropic Lipid Cubic Phases for Biosensing Platforms

Liu,

Lewis,

di Lorenzo

et al. 2023

Langmuir

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Enzyme-based electrochemical biosensors play an important role in point-of-care diagnostics for personalized medicine. For such devices, lipid cubic phases (LCP) represent an attractive method to immobilize enzymes onto conductive surfaces with no need for chemical linking. However, research has been held back by the lack of effective strategies to stably co-immobilize enzymes with a redox shuttle that enhances the electrical connection between the enzyme redox center and the electrode. In this study, we show that a monoolein (MO) LCP system doped with an amphiphilic redox mediator (ferrocenylmethyl)dodecyldimethylammonium bromide (Fc12) can be used for enzyme immobilization to generate an effective biosensing platform. Small-angle X-ray scattering (SAXS) showed that MO LCP can incorporate Fc12 while maintaining the Pn3m symmetry morphology. Cyclic voltammograms of Fc12/MO showed quasi-reversible behavior, which implied that Fc12 was able to freely diffuse in the lipid membrane of LCP with a diffusion coefficient of 1.9 ± 0.2 × 10–8 cm2 s–1 at room temperature. Glucose oxidase (GOx) was then chosen as a model enzyme and incorporated into 0.2%Fc12/MO to evaluate the activity of the platform. GOx hosted in 0.2%Fc12/MO followed Michaelis–Menten kinetics toward glucose with a K M and I max of 8.9 ± 0.5 mM and 1.4 ± 0.2 μA, respectively, and a linearity range of 2–17 mM glucose. Our results therefore demonstrate that GOx immobilized onto 0.2% Fc12/MO is a suitable platform for the electrochemical detection of glucose.

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