Properties of glucose sensors prepared by the electropolymerization of pyrroles containing phosphatidylcholine (II)

Yasuzawa, Mikito; Matsuki, Takashi; Mitsui, Hikari; Kunugi, Akira; Nakaya, Tadao

doi:10.1016/s0925-4005(99)00452-9

Cited by 21 publications

(20 citation statements)

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“…so-called biofouling which may include protein/platelet deposition, or the attachment of inflammatory response-related cells, is one of the major factors resulting in unpredictable and unexplainable behaviors of implanted glucose biosensors. The performance of implanted biosensors can greatly benefit from the use of more biocompatible outermost coatings such as hydrophilic alginate (Shichiri et al, 1989), phosphorylcholine-modified polyurethane (Yang et al, 2000), 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate (Nishida et al, 1995;Yasuzawa et al, 2000) and hydrogels (Suri et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Use of hydrogel coating to improve the performance of implanted glucose sensors

Wang

et al. 2008

Biosensors and Bioelectronics

101

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Section: Introductionmentioning

confidence: 99%

Use of hydrogel coating to improve the performance of implanted glucose sensors

Wang

et al. 2008

Biosensors and Bioelectronics

101

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“…Ortho isomer poly(o-phenylenediamine) of polyphenylenediamines is the most studied form, and is the subject of the present study. 38 Poly(o-phenylenediamine) films have been widely used on amperometric glucose, 26,30,[39][40][41][42][43][44][45] phenol, 46 L-lysine 47,48 and ethanol 49 biosensor for elimination of interference from electroactive species present in real samples. A literature search revealed that there were no reports of biosensor prepared by the polymerization conditions used in this study.…”

Section: (1)mentioning

confidence: 99%

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

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Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

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“…There is a growing interest in using electrochemically generated polymers, such as polypyrroles, in biosensor applications primarily as immobilization matrices because such allows the precise localization of enzymes and hence is ideal for enzyme deposition at miniature electrodes. [16][17][18][19][20][21][22][23][24][25][26] It seemed attractive to prepare N-substituted pyrrole polymers containing phosphatidylcholine, since the obtained electrogenerated film can be prospected to act as a hemocompatible material. The authors have reported that such pyrroles are useful for the immobilization of glucose oxidase (GOx) on platinum electrode and the obtained GOx-immobilized electrode functioned as a glucose sensor with good long-term stability.…”

Section: Introductionmentioning

confidence: 99%

“…The authors have reported that such pyrroles are useful for the immobilization of glucose oxidase (GOx) on platinum electrode and the obtained GOx-immobilized electrode functioned as a glucose sensor with good long-term stability. 24 This paper is mainly concerned with the synthesis and properties of such polymers. The characterization of the polymers was based on their IR spectra and elemental analysis.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Electropolymerization of Phosphorylcholine-Containing Pyrroles and Their Hemocompatible Properties

et al. 2010

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A series of N-substituted pyrroles having phosphorylcholine with different methylene chain lengths between pyrrole group and phosphorylcholine group were synthesized and their electropolymerizations were performed in aqueous solution. The methylene chains were trimethylene (n = 3), pentamethylene (n = 5), nonamethylene (n = 9), and undecamethylene (n = 11), for 3-(1-pyrrolyl)propyl-2-(trimethylammonium)ethyl phosphate (5a), 5-(1-pyrrolyl)pentyl-2-(trimethylammonium)ethyl phosphate (5b), 9-(1-pyrrolyl)nonyl-2-(trimethylammonium)ethyl phosphate (5c), and 11-(1-pyrrolyl)undecyl-2-(trimethylammonium)ethyl phosphate (5d), respectively. Although electropolymerized films were produced from all pyrrole derivatives, thick and black polymer films were prepared from 5a, 5b and 5c. The pyrrole derivative with long methylene-chain 5d provided only colorless or slightly blackish thin film. Hemocompatibilities of the polymers from 5a, 5b and 5c were evaluated by platelet rich plasma (PRP) contacting studies and scanning electron microscopy (SEM) observations. The results indicated that these polymers have excellent hemocompatibility. Scheme 1 General reaction route to synthesize 5a -5d.

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Properties of glucose sensors prepared by the electropolymerization of pyrroles containing phosphatidylcholine (II)

Cited by 21 publications

References 1 publication

Use of hydrogel coating to improve the performance of implanted glucose sensors

Use of hydrogel coating to improve the performance of implanted glucose sensors

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Synthesis and Electropolymerization of Phosphorylcholine-Containing Pyrroles and Their Hemocompatible Properties

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