Properties of NADH oxidase fromLactobacillus delbrueckii sspbulgaricus

Yi, Xin; Kot, Eva; Bezkorovainy, Anatoly

doi:10.1002/(sici)1097-0010(199812)78:4<527::aid-jsfa149>3.0.co;2-l

Cited by 11 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…delbrueckii subsp. bulgaricus ATCC 11842 can reduce oxygen to H 2 O 2 with an NADH-dependent oxidase to eliminate or lower the level of oxygen (460). The same was found for Lb.…”

Section: Stress Associated With Technological Productionsupporting

confidence: 53%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

View full text Add to dashboard Cite

SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

show abstract

“…delbrueckii subsp. bulgaricus ATCC 11842 can reduce oxygen to H 2 O 2 with an NADH-dependent oxidase to eliminate or lower the level of oxygen (460). The same was found for Lb.…”

Section: Stress Associated With Technological Productionsupporting

confidence: 53%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

View full text Add to dashboard Cite

show abstract

“…Survival and growth of lactic acid bacteria under oxic conditions require activation of the NADH oxidase/peroxidase system (Sakamoto et al 1996;Yi et al 1998). NADH oxidase is thought to control the intracellular redox balance and protect cells from H 2 O 2 toxicity.…”

Section: Enzyme Activities In Relation To O 2 Sensitivitysupporting

confidence: 90%

Superoxide dismutase plays an important role in the survival of Lactobacillus sake upon exposure to elevated oxygen

Amanatidou¹,

Bennik²,

Gorris³

et al. 2001

Archives of Microbiology

View full text Add to dashboard Cite

In this study, the responses of two Lactobacillus sake strains to elevated oxygen concentrations at 8 degrees C were investigated. L. sake DSM 6333 (L. sake(sens)), unlike L. sake NCFB 2813 (L. sake(ins)), showed a low growth rate in the presence of 90% O(2) and a rapid loss in viability shortly after entry into stationary phase. The steady-state cytosolic superoxide radical (O(2)(-)) concentration in L. sake(sens) was 0.134 microM and in the oxygen-insensitive mutant LSUV4 it was 0.013 microM. The nine- to ten-fold decrease in the rate of O(2)(-) elimination in L. sake(sens) indicates the significance of the O(2)(-)-scavenging system in protecting against elevated O(2). The superoxide dismutase (SOD) activity was 10- to 20-fold higher in L. sake(ins) than in L. sake(sens), depending on the growth phase. An oxygen-insensitive mutant of L. sake(sens), designated as strain LSUV4, had a ten-fold higher SOD activity than the wild-type strain, which likely restored its oxygen tolerance. Damage to proteins in L. sake(sens) was evidenced by the increased protein carbonyl content and reduced activities of the [Fe-S]-cluster-containing enzymes fumarase and fumarate reductase. This study forms a physiological basis for understanding the significance of elevated oxygen stress as an additional method for inhibition of microbial growth in relation to food preservation.

show abstract

“…Previously, it has been suggested that L. delbrueckii, a close relative of L. johnsonii, produces H 2 O 2 via an NADH-dependent reaction that is enhanced by the addition of a flavin source (8,35,36). To identify the protein and gene involved in such proposed enzymatic reaction in L. johnsonii, we initiated its purification, using enzyme activity assays to track the enzyme during purification.…”

Section: Resultsmentioning

confidence: 99%

“…It has been established that these species endure oxidative stress as a consequence of endogenously produced H 2 O 2 (36,51,52). The enzymes identified here have characteristics that are in agreement with those observed previously in strains of the L. acidophilus group, including that the enzyme reaction consumes NADH, uses flavin as a cofactor, is constitutively expressed, and is not produced in response to molecular oxygen (8,35,36). In addition, the maximal reaction rate measured in cell extracts of L. johnsonii is in the same order of magnitude as the rate previously reported for L. delbrueckii (36).…”

Section: Discussionmentioning

confidence: 99%

“…bulgaricus, Lactobacillus kefiranofaciens, and Lactobacillus helveticus). Although many studies have reported on the H 2 O 2 production by species of the L. acidophilus group, our understanding of the enzymatic reactions and mechanisms underlying these observations remains limited to the notion that NADH and flavin are involved in the reaction (35,36) and that it is catalyzed by a protein that is constitutively expressed (8). The enzymes that catalyze the H 2 O 2 -generating reactions remain uncharacterized, to date.…”

mentioning

confidence: 99%

See 1 more Smart Citation

H ₂ O ₂ Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase

Hertzberger

Arents

Dekker

et al. 2014

Appl Environ Microbiol

141

106

View full text Add to dashboard Cite

e Hydrogen peroxide production is a well-known trait of many bacterial species associated with the human body. In the presence of oxygen, the probiotic lactic acid bacterium Lactobacillus johnsonii NCC 533 excretes up to 1 mM H 2 O 2 , inducing growth stagnation and cell death. Disruption of genes commonly assumed to be involved in H 2 O 2 production (e.g., pyruvate oxidase, NADH oxidase, and lactate oxidase) did not affect this. Here we describe the purification of a novel NADH-dependent flavin reductase encoded by two highly similar genes (LJ_0548 and LJ_0549) that are conserved in lactobacilli belonging to the Lactobacillus acidophilus group. The genes are predicted to encode two 20-kDa proteins containing flavin mononucleotide (FMN) reductase conserved domains. Reductase activity requires FMN, flavin adenine dinucleotide (FAD), or riboflavin and is specific for NADH and not NADPH. The K m for FMN is 30 ؎ 8 M, in accordance with its proposed in vivo role in H 2 O 2 production. Deletion of the encoding genes in L. johnsonii led to a 40-fold reduction of hydrogen peroxide formation. H 2 O 2 production in this mutant could only be restored by in trans complementation of both genes. Our work identifies a novel, conserved NADH-dependent flavin reductase that is prominently involved in H 2 O 2 production in L. johnsonii.

show abstract

Properties of NADH oxidase fromLactobacillus delbrueckii sspbulgaricus

Cited by 11 publications

References 10 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Superoxide dismutase plays an important role in the survival of Lactobacillus sake upon exposure to elevated oxygen

H ₂ O ₂ Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase

Contact Info

Product

Resources

About

Properties of NADH oxidase fromLactobacillus delbrueckii sspbulgaricus

Cited by 11 publications

References 10 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Superoxide dismutase plays an important role in the survival of Lactobacillus sake upon exposure to elevated oxygen

H 2 O 2 Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase

Contact Info

Product

Resources

About

H ₂ O ₂ Production in Species of the Lactobacillus acidophilus Group: a Central Role for a Novel NADH-Dependent Flavin Reductase