Properties of ornithine decarboxylase-antizyme from mammary gland of lactating rats

Changes in ornithine decarboxylase and antizyme activities in developing mouse brain

“…(2) its inhibitory effect on ODC can be reversed by antizyme inhibitor; (3) its Mr (26000) agrees reasonably with reported values for antizymes from a variety of sources, such as rat liver (Heller et al, 1976;Fujita et al, 1984;Kitani & Fujisawa, 1984), mouse kidney (Hayashi et al, 1986), H-35 cells (Fong et al, 1976), L-1210 and mouse neuroblastoma cells (Heller et al, 1976), RK13 rabbit kidney cells (Murakami et al, 1986) and rat lymphoma cells (Richards et al, 1986), although antizymes of Mr 50000-90000 have also been found in rat thymus (Bishop et al, 1985), rat mammary gland (Hu & Brosnan, 1987) and rat lymphoma cells (Richards et al, 1986). Although we did not examine another important criterion for antizyme, namely its inducibility by polyamines (Fong et al, 1976), Laitinen et al (1985b) observed that intraventricular administration of spermine caused some increase in the amounts of free and total antizyme activities in mouse brain.…”

Rapid and regulated degradation of ornithine decarboxylase

“…Thus, antizymes of 50 kDa have been reported to be present in rat thymus [150] and mammary gland [151], and even antizyme of 90 kDa was observed in lymphoma cells [152]. Northern-blot analysis indicated that thymus extract contained a single hybridizable antizyme mRNA of a size identical with that of rat liver antizyme mRNA [73].…”