Properties of porcine white adipose tissue and liver mitochondrial subpopulations

Koekemoer, Trevor; Oelofsen, Willem

doi:10.1016/s1357-2725(01)00064-4

Cited by 21 publications

(26 citation statements)

References 45 publications

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“…Nevertheless, the current results of mtDNA give further support to the idea that mtDNA copy number is greater in the heavy fractions than in the light ones [48], suggesting an active mtDNA replication throughout the mitochondrial differentiation process, which reflects the connection between mitochondrial subpopulations and the mitochondrial proliferation-differentiation process in cells [39][40][41][42][43].…”

Section: Discussionsupporting

confidence: 76%

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Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Alcolea¹,

Colom²,

Lladó³

et al. 2006

Cell Physiol Biochem

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In the current study, the mitochondrial proliferationdifferentiation process was investigated in rat embryo during the placentation process, straight after organogenesis, when there is an important oxidative metabolism activation. For this purpose, on gestational days 11, 12 and 13 we studied the mitochondrial DNA (mtDNA) content and the relative gene expression of proteins involved in mtDNA replication (mitochondrial single strand DNA binding protein (mtSSB)), mtDNA transcription (mitochondrial transcription factor A (TFAM)), as well as in mitochondrial function (cytochrome c oxidase subunit I (COXI)). The results indicated that during placentation important changes in mitochondrial proliferation-differentiation process take place in rat embryo. There is a great decrease in cellular mtDNA content and a rise in the ratio between TFAM and mtDNA accompanied by an increase in COXI gene expression. Thus, we can conclude that on gestational day 13 mitochondrial differentiation predominates over mitochondrial proliferation in embryo cells. Besides, our work reveals that in a physiological condition such as embryonic development the TFAM levels change in order to regulate the transcriptional activity of mtDNA.

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Section: Discussionsupporting

confidence: 76%

“…Mitochondrial DNA content Quantification of mtDNA content was performed in both homogenate and mitochondrial subpopulations of rat embryo (Figure 1 and 2), as an indicator of mitochondrial number and therefore, of mitochondrial proliferation [43,53].…”

Section: Mitochondrial Subpopulation Protein Contentmentioning

confidence: 99%

Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Alcolea¹,

Colom²,

Lladó³

et al. 2006

Cell Physiol Biochem

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“…In the present study, comparisons between mitochondria from control male and female rats confirmed our previous findings but also highlighted differences in liver mitochondrial number between sexes. Considering that mtDNA content is indicative of number of mitochondria (9,23), female rats showed a lower mitochondrial content per cell compared with males. However, the content of protein per mitochondria, and its specific O 2 consumption, indicates a greater machinery or differentiation state of these mitochondria in agreement with the higher membrane potential previously described in female liver mitochondria (20).…”

Section: Sexual Dimorphism In Liver Mitochondriamentioning

confidence: 99%

Sexual dimorphism in liver mitochondrial oxidative capacity is conserved under caloric restriction conditions

Valle¹,

Guevara²,

García-Palmer³

et al. 2007

American Journal of Physiology-Cell Physiology

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Valle A, Guevara R, García-Palmer FJ, Roca P, Oliver J. Sexual dimorphism in liver mitochondrial oxidative capacity is conserved under caloric restriction conditions. Am J Physiol Cell Physiol 293: C1302-C1308, 2007. First published July 25, 2007; doi:10.1152/ajpcell.00203.2007.-Caloric restriction (CR) without malnutrition has been shown to increase maximal life span and delay the rate of aging in a wide range of species. It has been proposed that reduction in energy expenditure and oxidative damage may explain the life-extending effect of CR. Sex-related differences also have been shown to influence longevity and energy expenditure in many mammalian species. The aim of the present study was to determine the sex-related differences in rat liver mitochondrial machinery, bioenergetics, and oxidative balance in response to short-term CR. Mitochondria were isolated from 6-mo-old male and female Wistar rats fed ad libitum or subjected to 40% CR for 3 mo. Mitochondrial O 2 consumption, activities of the oxidative phosphorylation system (complexes I, III, IV, and V), antioxidative activities [MnSOD, glutathione peroxidase (GPx)], mitochondrial DNA and protein content, mitochondrial H 2O2 production, and markers of oxidative damage, as well as cytochrome C oxidase and mitochondrial transcription factor A levels, were measured. Female rats showed a higher oxidative capacity and GPx activity than males. This sexual dimorphism was not modified by CR. Restricted rats showed slightly increased oxygen consumption, complex III activity, and GPx antioxidant activity together with lower levels of oxidative damage. In conclusion, the sexual dimorphism in liver mitochondrial oxidative capacity was unaffected by CR, with females showing higher mitochondrial functionality and ROS protection than males.

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“…Samples were centrifuged at 10,000 rpm for 5 min and the resulting supernatant was used for amplification. A quantitative PCR assay was adapted to the LightCycler technology from previous methods [28,29]. PCR was performed to amplify …”

Section: Glutathione Assaymentioning

confidence: 99%

Differences in Mitochondrial Function and Antioxidant Systems between Regions of Human Glioma

Santandreu

Brell

Gene

et al. 2008

Cell Physiol Biochem

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Metabolic features and oxidative stress have been extensively studied in cancer cells. However, comparative studies between cancer cell populations that coexist in human neoplastic tissue are not frequently available. The aim of the present study was to characterize markers of oxidative status and mitochondrial function in center vs. periphery of human fresh glioma samples; therefore, antioxidant systems, oxidative stress and mitochondrial parameters were assessed in gross total resections of gliomas. Mitochondrial protein and mitochondrial DNA content, enzymatic activities of mitochondrial oxidative and phosphorylative system, antioxidant mechanisms, mitochondrial H₂O₂ production, oxygen consumption and cellular oxidative damage were measured in human gliomas. Concentric regions of human glioma tissue showed similar mitochondrial structural markers; conversely, the functionality of their isolated mitochondria was significantly different. In this way, the tumor periphery exhibited higher respiratory rate and fewer antioxidant systems than tumor center. Our results have expanded previous investigations, which report the presence of cell populations with different oxidative susceptibility in human brain tumor samples. This is, to our knowledge, the first study to investigate metabolic differences in concentric regions of gross total resections of glioma. Interestingly, the cancer cell population that exhibits an increased antioxidant capacity within the tumor mass might be responsible for tumor resistance to chemotherapy and radiotherapy.

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Properties of porcine white adipose tissue and liver mitochondrial subpopulations

Cited by 21 publications

References 45 publications

Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Mitochondrial Transcription Factor A (TFAM) is Increased in Rat Embryo During Placentation and Associated with Mitochondrial Differentiation

Sexual dimorphism in liver mitochondrial oxidative capacity is conserved under caloric restriction conditions

Differences in Mitochondrial Function and Antioxidant Systems between Regions of Human Glioma

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