Properties of purified salivary ribonuclease, and salivary ribonuclease levels in children with cystic fibrosis and in heterozygous carriers

Bardoń, A; Shugar, David

doi:10.1016/0009-8981(80)90051-0

Cited by 24 publications

(17 citation statements)

References 18 publications

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“…Degraded RNA poses a challenge to microarray‐based transcriptome profiling and validation via quantitative PCR . The current T7 IVT amplification method relies on the presence of 3′poly(A) tail or random priming, which may severely shorten transcripts and over‐represent long transcripts .…”

Section: Discussionmentioning

confidence: 99%

Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility

Hidayat

Milne

Cullinan

et al. 2017

J of Periodontal Research

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Section: Discussionmentioning

confidence: 99%

Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility

Hidayat

Milne

Cullinan

et al. 2017

J of Periodontal Research

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“…Our data suggest that different persons have different concentrations and species of long RNAs, possibly because saliva harbors different microorganisms that have different amounts and types of endo-and exoribonucleases, which affect the stability and length of salivary RNA. Alternatively, but not mutually exclusively, there are at least 2 known types of human nucleases that are active in saliva (8,11 ), and different individuals may have different amounts of these nucleases. The presence of partially degraded RNA suggests that endonucleases rather than exonucleases are primarily involved in the degradation of salivary RNA, because exonucleases are usually processive, degrading the target RNA substrates completely or giving rise to significant degradation on one end of the mRNA, as has been shown for plasma RNA (26 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of RNA in Saliva

et al. 2006

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Background:We have previously shown that human mRNAs are present in saliva and can be used as biomarkers of oral cancer. In this study, we analyzed the integrity, sources, and stability of salivary RNA. Methods: We measured the integrity of salivary RNA with reverse transcription followed by PCR (RT-PCR) or RT-quantitative PCR (RT-qPCR). To study RNA entry sites into the oral cavity, we used RT-PCR analysis of salivary RNA from the 3 major salivary glands, gingival crevice fluid, and desquamated oral epithelial cells. We measured stability of the salivary ␤-actin mRNA by RT-qPCR of salivary RNA incubated at room temperature for different periods of time. We measured RNA association with other macromolecules by filtering saliva through pores of different sizes before performing RT-qPCR. To assess RNA-macromolecule interaction, we incubated saliva with Triton X-100 for different periods of time before performing RT-qPCR. Results: In most cases, we detected partial-to fulllength salivary mRNAs and smaller amounts of middle and 3 gene amplicons compared with the 5. RNA was present in all oral fluids examined. Endogenous salivary ␤-actin mRNA degraded more slowly than exogenous ␤-actin mRNA, with half-lives of 12.2 and 0.4 min, respectively (P <0.001). Salivary RNA could not pass through 0.22 or 0.45 m pores. Incubation of saliva with Triton X-100 accelerated degradation of salivary RNA. Conclusions: Saliva harbors both full-length and partially degraded forms of mRNA. RNA enters the oral cavity from different sources, and association with macromolecules may protect salivary RNA from degradation.

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“…The salivary types were well defined as to origin and flow rate and it was therefore hoped that the results would give us more information about our earlier findings in whole saliva (4)(5)(6).…”

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confidence: 98%

“…RNAase is easy to obtain and analyse in different body fluids (3)(4)(5)(6)(7), e.g. the saliva (3)(4)(5)(6).…”

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confidence: 99%

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Ribonuclease in Different Types of Saliva from Cystic Fibrosis Patients

et al. 1985

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The flow rate, the activity of ribonuclease (RNAase) and the concentration of protein were determined in whole saliva and in parotid and submandibular saliva from patients with cystic fibrosis (CF) and from healthy controls, both before and after stimulation of the salivary secretion. Lower flow rates were found in all types of saliva from CF-patients than in control saliva. Increased activity of RNAase was found in CF saliva, both before and after stimulation of the secretion. The concentration of protein was also increased, but to a lesser degree. No correlation was found between either RNAase activity or protein concentration and severity of the disease or age of the children. It is therefore unlikely that these disturbances are secondary to progression of the disease. Considerable variations in RNAase activity and protein concentration were observed, especially within the CF group, and therefore despite the significant increase in these variables in CF their estimation is of limited diagnostic value.

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Properties of purified salivary ribonuclease, and salivary ribonuclease levels in children with cystic fibrosis and in heterozygous carriers

Cited by 24 publications

References 18 publications

Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility

Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility

Characterization of RNA in Saliva

Ribonuclease in Different Types of Saliva from Cystic Fibrosis Patients

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