Properties of rabbit antiragweed and antihorseradish peroxidase homocytotropic antibodies

The formation of homocytotropic antibodies (IgE) as determined by immunochemical characteristics against penicilloyl in rabbits was shown. The production of such antibodies to azidocilloyl-human serum albumin (AzO₁₀-HSA) in alum was found optimal using 1 mg of antigen in the tested range of 0.01–8 mg. At the lowest dose (0.01 mg) only haemagglutinating but no IgE antibodies were formed. Immunization with azidocilloyl-bovine γ-globulin (AzO₉-BGG) resulted in a slower increase in antibody levels than was caused by AzO₁₀-HSA. Univalent benzylpenicilloyl-ε-aminocaproate or ampicillin given together with the antigen upon immunization decreased the levels of penicilloyl specific IgE and haemagglutinating antibodies, but induced the formation of IgE antibodies against the carrier molecule. Further, the administration of penicilloyl specific IgG antibodies diminished formation of both IgE and haemagglutinating antibodies, but no antibodies specific for the carrier were formed. The usefulness of this animal model for the experimental study of penicillin allergy is discussed.

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“…These rabbit antibodies had properties similar to the human IgE anti bodies, which is in agreement with previous reports [1,8].…”

Section: Discussionsupporting

confidence: 81%

Formation of Homocytotropic Antibodies with Penicilloyl Specificity in the Rabbit

Rodell¹,

Ahlstedt²,

Olsson³

et al. 1976

Int Arch Allergy Immunol

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Rabbit homocytotrophic antibody response to horseradish peroxidase: The role of contaminating antigens

1975

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Detection of antibody-forming cells and humoral antibody to horseradish peroxidase

Burke

Harris

Stollar

et al. 1979

Journal of Immunological Methods

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Properties of rabbit antiragweed and antihorseradish peroxidase homocytotropic antibodies

Cited by 3 publications

References 26 publications

Formation of Homocytotropic Antibodies with Penicilloyl Specificity in the Rabbit

Formation of Homocytotropic Antibodies with Penicilloyl Specificity in the Rabbit

Rabbit homocytotrophic antibody response to horseradish peroxidase: The role of contaminating antigens

Detection of antibody-forming cells and humoral antibody to horseradish peroxidase

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