Properties of recombinant endo-β-1,6-glucanase from Trichoderma harzianum and its application in the pustulan hydrolysis

Volkov, Pavel V.; Рубцова, Е. А.; Рожкова, А. М.; Синицына, О. А.; Зоров, И. Н.; Kondratyeva, E. G.; Sinitsyn, A. P.

doi:10.1016/j.carres.2020.108211

Cited by 9 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Excessive exposure to the gene encoding β-1,3glucanase at the early stages of mycoparasitism was discovered during the expression of T. harzianum and Fusarium solani transcriptome interaction [38]. The capacity to regulate bio-T. virens by Pythium ultimum is also reduced when the Tv bgn-3-gene encoding the enzyme β-1,6-glucanase is removed [46]. Trichoderma soluble in fluid cultures having a wall of lyophilised cells of the pathogenic Fusarium culmorum strain as the only carbon source has been reported to have increased chitinolytic and glucanolytic activity [47].…”

Section: Synthesis Of Cell Wall Degrading Compounds (Cwdcs)mentioning

confidence: 99%

“…Antibiosis was observed in Trichoderma, which synthesises a variety of antagonistic compounds that break down the cell wall, e.g. pectinase, xylanase, cellulase, lipase, amylase, glucanase and protease, as well as other flexible metabolites or volatile compounds (VOCs) like antibiotics such as trichodermin, gliotoxin, viridin, peptaibols [46]. These and several other secondary metabolites have been isolated and characterised, with over 370 belonging to different groups of potent antagonists [41,52].…”

Section: Production Of Antibiotics and Other Antifungal Compoundsmentioning

confidence: 99%

“…However, sugar and sucrose levels are high in Trichoderma [40] and are utilised as a carbon source [34]. Trichoderma has superior competition and nutrient absorption ability compared to other fungi genera [46]. This process is linked to gluconic, citric, and fumaric acid production, which reduces soil pH and increases phosphate and microelement (iron, manganese, and magnesium) synthesis [76].…”

Section: Competition For Vital Nutrients and Spacementioning

confidence: 99%

See 2 more Smart Citations

Trichoderma: A Review of Its Mechanisms of Action in Plant Disease Control

Oyesola,

Tonjock,

Bello

et al. 2024

Preprint

View full text Add to dashboard Cite

Trichoderma has been widely studied for its potential as a biocontrol agent against plant pathogenic organisms. Trichoderma's biological control mechanisms include competition, modification of environmental conditions, antibiosis, induction of plant defensive mechanisms, and mycoparasitism. Trichoderma species are known to produce a variety of secondary metabolites that have antifungal activity. These metabolites include peptaibols, gliotoxin, and trichokonins. Trichoderma also produces chitinases and β-1,3-glucanases that can degrade the cell walls of fungal pathogens. In addition to direct antagonism against fungal pathogens, Trichoderma can also induce systemic or localised resistance in plants, which is achieved through the production of elicitors such as chitin oligosaccharides and β-glucans that activate plant defence responses. Trichoderma can also form mutualistic associations with plants. In these associations, Trichoderma colonises the roots of plants and promotes plant growth by increasing nutrient uptake and inducing systemic resistance. Using Trichoderma as a biocontrol agent has several advantages over conventional crop protection techniques based on applying synthetic pesticides.

show abstract

Section: Synthesis Of Cell Wall Degrading Compounds (Cwdcs)mentioning

confidence: 99%

Section: Production Of Antibiotics and Other Antifungal Compoundsmentioning

confidence: 99%

Section: Competition For Vital Nutrients and Spacementioning

confidence: 99%

See 1 more Smart Citation

Trichoderma: A Review of Its Mechanisms of Action in Plant Disease Control

Oyesola,

Tonjock,

Bello

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Similarly, most characterized endo-glucanases hydrolyze polysaccharides into cello-oligosaccharides, such as G2, G3, G4 and G5 [32,33,34,24]. However, some glucanases have product profiles unlike normal endo-acting glucanases.…”

Section: A C C E P T E Dmentioning

confidence: 99%

Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen

Zhu,

Bai,

et al. 2024

Anim Biosci

View full text Add to dashboard Cite

This study aimed to identify and characterize a novel endo-β-glucanase, IDSGLUC9-4, from the rumen metatranscriptome of Hu sheep. Methods:A novel endo-β-glucanase, IDSGLUC9-4, was heterologously expressed in Escherichia coli and biochemically characterized. The optimal temperature and pH of recombinant IDSGLUC9-4 were determined. Subsequently, substrate specificity of the enzyme was assessed using mixed-linked glucans including barley β-glucan and Icelandic moss lichenan. Thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) analyses were conducted to determine the products released from polysaccharides and cello-oligosaccharides substrates. Results:The recombinant IDSGLUC9-4 exhibited temperature and pH optima of 40 °C and pH 6.0, respectively. It exclusively hydrolyzed mixed-linked glucans, with significant activity observed for barley β-glucan (109.59 ± 3.61 μmol•mg -1 •min -1 ) and Icelandic moss lichenan (35.35 ± 1.55 μmol•mg -1 •min -1 ). TLC and HPLC analyses revealed that IDSGLUC9-4 primarily released cellobiose, cellotriose, and cellotetraose from polysaccharide substrates. Furthermore, after 48 h of reaction, IDSGLUC9-4 removed most of the glucose, indicating transglycosylation activity alongside its endo-glucanase activity.

show abstract

“…β-Glucanases, together with microbial cell wall β-glucans, play a major role in host–pathogen interactions in many pathogenic species, to the extent that β-glucanases have been used as biosensors for the detection of pathogens [ 18 ]. β-1,6-Glucanases are produced by bacteria growing on fungal cell walls as carbon source [ 19 ], but they are also present in fungi [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Zerva

Mohammadi

Dimopoulos

et al. 2023

Waste Biomass Valor

View full text Add to dashboard Cite

Alternative sweeteners, such as steviol glucosides from the plant Stevia rebaudiana Bertoni, are becoming increasingly popular for the design of next-generation foodstuffs. However, the bitter aftertaste of native steviol glucosides is one of the main reasons behind consumer reluctance towards stevia-containing products. Biocatalysis could be a sustainable solution to this problem, through addition of glucosyl moieties to the molecule. Glycoside hydrolases are enzymes performing transglycosylation reactions, and they can be exploited for such modifications. In the present work, the commercial β-glucanase Finizym 250L® was employed for the transglycosylation of stevioside. After optimization of several reaction parameters, the maximal reaction yield obtained was 19%, with barley β-glucan as the glycosyl donor. With the aim to develop a sustainable process, β-glucan extracts from different fungal sources were prepared. Pulsed Electric Field pretreatment of mycelial biomass resulted in extracts with higher β-glucan content. The extracts were tested as alternative glucosyl donors, reaching up to 15.5% conversion yield, from Pleurotus-extracted β-glucan. Overall, in the present work a novel enzymatic process for the modification of stevioside is proposed, with concomitant valorization of β-glucans extracted from fungal biomass, potentially generated as a byproduct from other applications, in concert with the principles of circular economy. Graphical Abstract

show abstract

Properties of recombinant endo-β-1,6-glucanase from Trichoderma harzianum and its application in the pustulan hydrolysis

Cited by 9 publications

References 32 publications

Trichoderma: A Review of Its Mechanisms of Action in Plant Disease Control

Trichoderma: A Review of Its Mechanisms of Action in Plant Disease Control

Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Contact Info

Product

Resources

About