Properties of secretory acid phosphatase from lupin roots under phosphorus-deficient conditions

Wasaki, Jun; Ando, Michiko; Ozawa, Keisuke; Omura, Masanori; Osaki, Mitsuru; Ito, Hiroyuki; Matsui, Hirokazu; Tadano, Toshiaki

doi:10.1007/978-94-009-0047-9_87

Cited by 22 publications

(21 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results suggest that APase is synthesized as secretory protein mainly in mature proteoid roots, and is subsequently released into the rhizosphere. Similarly, Wasaki et al (1997) reported predominant expression of a cDNA clone with 76% homology to purple APase of Phaseolus vulgaris in lateral roots of P-de®cient white lupin.…”

Section: Discussionmentioning

confidence: 92%

Physiological adaptations to phosphorus deficiency during proteoid root development in white lupin

Neumann

Massonneau

Martinoia

et al. 1999

Planta

394

242

View full text Add to dashboard Cite

Release of large amounts of citric acid from specialized root clusters (proteoid roots) of phosphorus (P)-de®cient white lupin (Lupinus albus L.) is an ecient strategy for chemical mobilization of sparingly available P sources in the rhizosphere. The present study demonstrates that increased accumulation and exudation of citric acid and a concomitant release of protons were predominantly restricted to mature root clusters in the later stages of P de®ciency. Inhibition of citrate exudation by exogenous application of anion-channel blockers such as ethacrynic-and anthracene-9-carboxylic acids may indicate involvement of an anion channel. Phosphorus-de®ciency-induced accumulation and subsequent exudation of citric acid seem to be a consequence of both increased biosynthesis and reduced metabolization of citric acid in the proteoid root tissue, indicated by increased in-vitro activity and enzyme protein levels of phosphoenolpyruvate carboxylase (EC 4.1.1.31), and reduced activity of aconitase (EC 4.2.1.3) and root respiration. Similar to citric acid, acid phosphatase, which is secreted by roots and involved in the mobilization of the organic soil P fraction, was released predominantly from proteoid roots of P-de®cient plants. Also 33 Pi uptake per unit root fresh-weight was increased by approximately 50% in juvenile and mature proteoid root clusters compared to apical segments of non-proteoid roots. Kinetic studies revealed a K m of 30.7 lM for Pi uptake of non-proteoid root apices in P-sucient plants, versus K m values of 8.5±8.6 lM for non-proteoid and juvenile proteoid roots under P-de®cient conditions, suggesting the induction of a high-anity Pi-uptake system. Obviously, P-de®ciency-induced adaptations of white lupin, involved in P acquisition and mobilization of sparingly available P sources, are predominantly con®ned to proteoid roots, and moreover to distinct stages during proteoid root development.Dedicated to the memory of Prof. Horst Marschner, who initiated this cooperation project Abbreviations: APase = acid phosphatase; P = phosphorus; Pi = inorganic phosphate; PEPC = phosphoenolpyruvate carboxylase

show abstract

Section: Discussionmentioning

confidence: 92%

Physiological adaptations to phosphorus deficiency during proteoid root development in white lupin

Neumann

Massonneau

Martinoia

et al. 1999

Planta

394

242

View full text Add to dashboard Cite

show abstract

“…The action of this enzyme under P-deficient conditions implies the increase in hydrolysis of organic P in order to create more free inorganic P (Ruiz, Belakbir, and Romero 1996). Wasaki et al (1997) observed that the P deficiency induced the synthesis of phosphatase acid in the roots of lupine plants (Lupinus polyphyllus). Römer and Fahnin (1998) indicated that phosphatase activity in the root was augmented when the P status diminished in the shoot of endogamic lines of Lolium multiflorum.…”

Section: Bioindicators Of Pmentioning

confidence: 98%

Physiological and Nutritional Evaluation of the Application of Phosphite as a Phosphorus Source in Cucumber Plants

Constán-Aguilar

Sánchez-Rodríguez

Rubio-Wilhelmi

et al. 2013

Communications in Soil Science and Plant Analysis

View full text Add to dashboard Cite

The aim of this study was to determine how different phosphite and phosphate rates applied together affect the growth and morphology parameters in cucumber plants as well as to characterize the nutritional and physiological state of phosphorus (P). Different foliar application rates of phosphite were applied to analyze subsequent biomass production, the relative growth rate, morphological parameters, P bioindicators, P forms, and P-use efficiency. The data suggest that the application of phosphite as a P fertilizer at a rate of ≥0.5 mM would be an appropriate and effective strategy under suboptimal conditions of phosphate in the growth medium, represented by 0.5 mM in our work, as it improved the growth parameters, number of flowers, leaf area, nutritional state of P, incorporation of P in structural organs, and P-use efficiency by the plant. The results indicate that the validity of the foliar use of phosphite as a P fertilizer in cucumber plants was subject to phosphate availability in the culture medium.

show abstract

“…The increase in H 2 O 2 -scavenging enzyme peroxidase might be attributed to rapid diffusivity of H 2 O 2 produced in the cytosol. The results on increased activity of phosphatase at excess Zn are substantiated by the work on lupin (Lupinus angustifolius L.) (Wassaki et al 1997), suggesting that P deficiency induces the synthesis of acid phosphatase.…”

Section: Resultsmentioning

confidence: 81%

Zinc Stress–Induced Changes in Biochemical Parameters and Oil Content of Mustard

Chatterjee

Khurana

2007

Communications in Soil Science and Plant Analysis

View full text Add to dashboard Cite

An experiment with mustard (Brassica campestris L.) cv T 59 was conducted in a glasshouse under controlled culture conditions to determine the effect of variable zinc (Zn) on biomass, reproductive yield, seed oil, and some aspects of metabolism. Plants were raised in refined sand, and Zn was administered at variable levels ranging from acute deficiency to excess (0.00065 to 65 mg Zn L 21 ). At 0.00065 mg Zn L 21 , symptoms of Zn deficiency were depression in growth, short internodes, chlorosis and necrosis of mature leaves, along with development of purple pigmentation at leaf margins, which later covered the entire lamina. Biomass, economic yield, and seed oil content were highest at 0.065 mg Zn L 21 . Zinc at less and more than 0.065 mg Zn L 21 reduced vegetative yield, pod and seed weight, concentration of chlorophyll (a and b), sugars, starch, and protein and elevated the concentration of phenols and activity of acid phosphatase and peroxidase in mustard leaves. The activity of carbonic anhydrase (except at 65 mg Zn L 21 ), aldolase, and leaf Zn increased with an increase in Zn from deficiency to excess whereas ribonuclease activity was related inversely to Zn supply. In addition to various changes in metabolism of mustard, Zn at higher levels (6.5 and 65 mg Zn L 21 ) induced symptoms of toxicity on old leaves as interveinal chlorosis, necrosis, and reduction in growth and leaf size. Phosphorus (P) content in leaves decreased concomitantly with an increase in Zn supply from 0.00065 to 65 mg Zn L 21 . The values of deficiency, threshold of deficiency, threshold of toxicity, and toxicity in leaves were respectively 18, 25, 200, and 380 mg Zn g 21 dry matter. The activity of aldolase and carbonic anhydrase appear to be reliable biological indicators for evaluating the Zn status of mustard.

show abstract

Properties of secretory acid phosphatase from lupin roots under phosphorus-deficient conditions

Cited by 22 publications

References 17 publications

Physiological adaptations to phosphorus deficiency during proteoid root development in white lupin

Physiological adaptations to phosphorus deficiency during proteoid root development in white lupin

Physiological and Nutritional Evaluation of the Application of Phosphite as a Phosphorus Source in Cucumber Plants

Zinc Stress–Induced Changes in Biochemical Parameters and Oil Content of Mustard

Contact Info

Product

Resources

About