1981
DOI: 10.1111/j.1348-0421.1981.tb00066.x
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Properties of the Biotin Transport System in Bifidobacterium breve N4

Abstract: Binding of biotin to resting cells of Bijidobacterium breve N4, which grew in a biotin-deficient medium, was independent of pH from I to 9 and of temperature below 50 C. It was not inhibited by metabolic inhibitors including sulfhydryl reagents, but it was inhibited by treatment with 80% ethanol or 5% trichloroacetic acid. It was also competitively inhibited by biotin-sulfone, but not by tetrahydrothiophene nor dethiobiotin. The binding constant was calculated to be 3.3 X 10 8 M-1•The amount of biotin unextrac… Show more

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