Properties of two amide‐based hydrophilic interaction liquid chromatography columns

Abstract

Hydrophilic interaction liquid chromatography is a separation technique suitable for the separation of moderately and highly polar compounds. Various stationary phases (SPs) for hydrophilic interaction liquid chromatography are commercially available. While the SPs based on the same type of ligand are available from different providers, they can display a distinct retention characteristics and separation selectivity. The current work is focused on characterization and comparison of the separation systems of two amide-based HPLC columns from two producers, i.e. XBridge Amide column and TSK gel Amide-80 column. Several characterization procedures (tests) were used to investigate the differences between these columns. The chromatographic behavior of selected analytes indicates that multimodal interactions are responsible for retention and separation on these columns. Multiple testing approaches were used in order to reveal subtle differences between the SPs. Both amide-based columns showed certain differences in retention, selectivity, and plate counts. Based on the tests used in this study, we conclude that the investigated columns provide a different degree of H-bonding interactions.

Protonation of polyaniline‐coated silica stationary phase affects the retention behavior of neutral hydrophobic solutes in reversed‐phase capillary liquid chromatography

“…Both retention (partitioning and adsorption) models illustrate the overall retention behavior and seem to corroborate the retention data of the neutral hydrophobic solutes used in this study. Therefore, based on the findings of this study and on the published literature , we presume a multimodal retention mechanism for PANI‐SiO 2 .…”

Chromatographic behavior of new deazapurine ribonucleosides in hydrophilic interaction liquid chromatography

“…, which indicated preference of partitioning. Thus, adsorption was preferred on modified columns (that can offer more interaction types ). The same results were obtained at buffer pH 4.70.…”

Effect of Buffer Constituents on Retention and Separation in Achiral and Chiral HPLC Systems with β-Cyclodextrin-Based Stationary Phase

“…The intercept c in the LFER equation is characteristic of the given system but it does not reflect any interaction [9][10][11][12][13]. The regression coefficients of the LFER equation were obtained from a series of retention measurements of the set of 32 structurally different solutes (they were selected to cover a wide range of chemical and structural properties) with known descriptors (see supporting information Table S1).…”