Prophylactic and Curative Effects of Thymoquinone against CCL4-Induced Hepatotoxicity in Rats

Khither, Hanane; Sobhi, Widad; Mosbah, Ahmed; Benboubetra, Mustapha

doi:10.9734/ejmp/2018/38830

Cited by 9 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, Thymohydroquinone, the reduced form of TQ acts as hydroxyl and superoxide radical's electron donner which attacks the cell membrane polyunsaturated fatty acids. This clarifies the powerful ROS scavenging capacity of TQ (Khither et al 2018). Additionally, El-Mahmoudy et al (2002) elucidated the ability of TQ to inhibit the formation of NO by downregulation of the inducible nitric oxide synthase expression.…”

Section: Discussionmentioning

confidence: 64%

Ameliorative effect of thymoquinone against acrylamide-induced hepatotoxicity in male rats

Ghonim¹

2023

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

Acrylamide (ACR) is a food toxicant detected in over-thermal cooked foods, especially carbohydrate-rich foodstuffs. ACR-induced toxicity in different organs in the body. The current study was planned to evaluate the possible ameliorative effect of Thymoquinone (TQ) against ACR-induced hepatotoxicity. Twenty-eight adult male rats were divided into four groups (seven rats/group) Control group received saline only, TQ group (20 mg/kg b.wt/day/orally), ACR group (20 mg/kg b.wt/day/orally), ACR+TQ group (administered (TQ 20 mg/kg b.wt) + (ACR 20 mg/kg b.wt) orally and once daily) for 28 days. Blood samples for serum separation and hepatic tissue specimens were taken on the last day of the experiment for determination of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total cholesterol (CHO), and triacylglycerols (TAG) in addition to hepatic oxidative markers including malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH). Also, histopathological and immunohistochemical examinations of the liver tissue were performed. ACR caused liver damage indicated by increasing the activity of marker enzymes, lipids profile, and reduction of the hepatic antioxidant enzymes CAT, SOD, and GPx activities and GSH content with marked elevation in MDA concentration. Also, ACR induced histopathological and immunohistochemical alterations in the hepatic tissue. TQ, however, reduced the liver tissue's oxidative stress and hepatic damage induced by ACR. Overall, the obtained findings showed that TQ's antioxidant mechanism reduced ACR-induced liver damage.

show abstract

Section: Discussionmentioning

confidence: 64%

Ameliorative effect of thymoquinone against acrylamide-induced hepatotoxicity in male rats

Ghonim¹

2023

Benha Veterinary Medical Journal

View full text Add to dashboard Cite

show abstract

“…Similarly, Abdel-Wahab (3) stated that thymoquinone (TQ) administration greatly normalized suppressed enzymatic and non-enzymatic antioxidant levels and reduced hepatic biomarkers and lipid peroxidation (3). Besides, studies have revealed that thymohydroquinone, the reduced form of TQ, shows strong antioxidant properties by returning electrons to hydroxyl radicals (OH) and superoxide radicals, which attack polyunsaturated fatty acids in the cell membrane (27). Based on these findings, we concluded that NS plays a key role in cellular protective mechanisms and exerts hepatoprotective effects by preventing or reducing tissue destruction caused by AAinduced ROS, increasing the level of GSH and SOD, and preventing the formation of lipid peroxidation products.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effects of Nigella Sativa oil against acrylamide-induced liver injury in rats

Hatipoğlu¹,

ÖZSAN

DÖNMEZ³

et al. 2023

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

This study was designed to investigate the possible protective efficacy of Nigella sativa essential oil against acrylamide induced liver toxicity. Four groups of 32 male Wistar albino rats were formed. Control group was used as a control. acrylamide was administered to the rats in Group AA by gavage at a daily dose of 40 mg/kg. 10mg/kg dose of Nigella sativa essential oil was administered intraperitoneally to the rats in Group NS. In Group AA+NS, daily acrylamide and Nigella sativa essential oil applications were carried out simultaneously as mentioned above. The trial period lasted 15 days. While acrylamide increased malondialdehyde levels in liver tissue, it decreased glutathione and superoxide dismutase levels. In addition, liver pro-inflammatory cytokine levels increased due to acrylamide administration, while anti-inflammatory cytokine levels decreased due to acrylamide administration.: Concomitant administration of Nigella sativa essential oil with acrylamide significantly reduced oxidative stress and inflammation in liver tissues. In conclusion, our findings emphasized that Nigella sativa essential oil has a potential therapeutic role to prevent acrylamide-induced hepatotoxicity.

show abstract

“…It is a member of bioflavonoid with antioxidant and anti-inflammatory, properties 3,4 . Our previous study revealed the power of TQ as prophylactic and also curative treatment against CCl4induced hepatotoxicity in male rats 6 .…”

Section: Introductionmentioning

confidence: 98%

Effect of Thymoquinone as Prophylactic Treatment Against CCl4-Induced Hepatotoxicity on Antioxidants Status

Khither

MOSBAH²,

MADOUI³

et al. 2020

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Objective: The present study aims to study the effect of thymoquinone as prophylactic treatment against CCl4-induced hepatotoxicity on antioxidants status. Methodology: Hepatotoxicity was induced in rats by intraperitoneal administration of 3 ml/kg, 1:1 (V/V) mixture of CCl4 and olive oil after treatment for 7 days with TQ, using two doses. The method consists of studying the antioxidant effect of thymoquinone pretreatment by measuring superoxide dismutase (SOD) and catalase (CAT) activities, with reduced glutathione level in both plasma and liver homogenate. Results: The results revealed that hepatotoxicity is accompanied by significant decrease (p ≤ 0.01) of SOD and CAT activities with GSH level, in both plasma and liver homogenate. While prophylactic treatment using TQ at doses of 02.5 and 05 mg/kg increase significantly the status of the antioxidants, as dose dependent manner, in both plasma and liver homogenate. Conclusion: The results of this study show that thymoquinone has an antioxidant effect when it used as prophylactic treatment against CCl4-induced hepatotoxicity. Keywords: Thymoquinone, hepatotoxicity, CCl4, prophylactic and antioxidant.

show abstract

Prophylactic and Curative Effects of Thymoquinone against CCL4-Induced Hepatotoxicity in Rats

Cited by 9 publications

References 0 publications

Ameliorative effect of thymoquinone against acrylamide-induced hepatotoxicity in male rats

Ameliorative effect of thymoquinone against acrylamide-induced hepatotoxicity in male rats

Hepatoprotective effects of Nigella Sativa oil against acrylamide-induced liver injury in rats

Effect of Thymoquinone as Prophylactic Treatment Against CCl4-Induced Hepatotoxicity on Antioxidants Status

Contact Info

Product

Resources

About