Prophylactic efficacy of Boerhavia diffusa L. aqueous extract in toluene induced reproductive and developmental toxicity in Drosophila melanogaster

Pb, Benil; Rani, Sreeja; Kim, Young Ock; Al-Ghamdi, Abdullah Ahmed; Elshikh, Mohamed S.; Al-Dosary, Monerah A.; Hatamleh, Ashraf Atef; Arokiyaraj, Selvaraj; Kim, Hak-Jae

doi:10.1016/j.jiph.2019.07.020

Cited by 14 publications

(24 citation statements)

References 22 publications

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“…The reproductive value of flies was measured using two fitness parameters: fecundity and fertility, which can be influenced by factors like fly genotype, body size, age, and mate, as well as environmental conditions [ 38 ]. In the present study, supplementation of A.L.…”

Section: Resultsmentioning

confidence: 99%

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

Kushalan

D’Souza

Aloysius

et al. 2022

IJERPH

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Curculigo orchioides is used in Indian and Chinese traditional medicinal systems for various health benefits. However, its toxicological effects are mostly unknown. This study assesses the potential toxicity of aqueous leaf (A.L.) extract of C. orchioides using Drosophila melanogaster as an experimental model. Preliminary phytochemical tests were followed by the Fourier transform infrared (FTIR) tests to identify the functional group in the A.L. extract of C. orchioides. Drosophila larvae/adults were exposed to varying concentrations of C. orchioides A.L. extract through diet, and developmental, lifespan, reproduction, and locomotory behaviour assays were carried out to assess the C. orchioides toxicity at organismal levels. The cellular toxicity of A.L. extract was examined by analysing the expression of heat shock protein (hsps), reactive oxygen species (ROS) levels, and cell death. The FTIR analysis showed the presence of functional groups indicating the presence of secondary metabolites like saponins, phenolics, and alkaloids. Exposure to A.L. extract during development resulted in reduced emergence and wing malformations in the emerged fly. Furthermore, a significant reduction in reproductive performance and the organism’s lifespan was observed when adult flies were exposed to A.L. extract. This study indicates the adverse effect of C. orchioides A.L. extract on Drosophila and raises concerns about the practice of indiscriminate therapeutic use of plant extracts.

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Section: Resultsmentioning

confidence: 99%

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

Kushalan

D’Souza

Aloysius

et al. 2022

IJERPH

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“…[ 27 ], may have very different toxic effects than applying the compound in solution or mixing it with the agar plates. The latter is for example applied in a recent DART study using fruit flies where exposure to toluene is established by adding the compound to agar medium [ 28 ]. For correct exposure the container must be kept closed so that the compound cannot escape [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Towards a reporting guideline for developmental and reproductive toxicology testing in C. elegans and other nematodes

Voet¹,

Teunis

Haar

et al. 2021

Toxicology Research

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Implementation of reliable methodologies allowing Reduction, Refinement, and Replacement (3Rs) of animal testing is a process that takes several decades and is still not complete. Reliable methods are essential for regulatory hazard assessment of chemicals where differences in test protocol can influence the test outcomes and thus affect the confidence in the predictive value of the organisms used as an alternative for mammals. Although test guidelines are common for mammalian studies, they are scarce for non-vertebrate organisms that would allow for the 3Rs of animal testing. Here, we present a set of 30 reporting criteria as the basis for such a guideline for Developmental and Reproductive Toxicology (DART) testing in the nematode Caenorhabditis elegans. Small organisms like C. elegans are upcoming in new approach methodologies for hazard assessment; thus, reliable and robust test protocols are urgently needed. A literature assessment of the fulfilment of the reporting criteria demonstrates that although studies describe methodological details, essential information such as compound purity and lot/batch number or type of container is often not reported. The formulated set of reporting criteria for C. elegans testing can be used by (i) researchers to describe essential experimental details (ii) data scientists that aggregate information to assess data quality and include data in aggregated databases (iii) regulators to assess study data for inclusion in regulatory hazard assessment of chemicals.

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“…Administration of the extract and standard control drug were done following previous methods. 46 , 49 , 64 , 65 For acute study models, 10-day-old flies in replicates of 10 flies per plastic fly vial (Genesee Scientific, 32-116, USA), were starved for 15 h to induce appetite, after which they were fed 1 g of standard yeast/cornmeal/agar media without extract (negative or mutant controls and wild-type controls for acute study), or 1 g of standard media mixed directly with 0.6 g/mL, 0.8 g/mL, or 1 g/mL extract (extract tests for acute study), or 1 g of standard media mixed with 0.3 mg/mL SV (standard controls for acute study), and for the acute tests, the flies were allowed to feed for 1, 2, or 3 h (each treatment was tested for 1-3 h) before being transferred into individual empty plastic fly vials (Genesee Scientific, 32-116, USA) that were capped with a cotton plug. The flies were allowed to sit undisturbed for 20 min before tests were conducted.…”

Section: Methodsmentioning

confidence: 99%

Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models

Ssempijja

Dare

Bukenya

et al. 2023

J Evid Based Complementary Altern Med

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Imperata cylindrica is a globally distributed plant known for its antiepileptic attributes, but there is a scarcity of robust evidence for its efficacy. The study investigated neuroprotective attributes of Imperata cylindrica root extract on neuropathological features of epilepsy in a Drosophila melanogaster mutant model of epilepsy. It was conducted on 10-day-old (at the initiation of study) male post-eclosion bang-senseless paralytic Drosophila ( parabss1) involved acute (1-3 h) and chronic (6-18 days) experiments; n = 50 flies per group (convulsions tests); n = 100 flies per group (learning/memory tests and histological examination). Administrations were done in 1 g standard fly food, per os. The mutant flies of study ( parabss1) showed marked age-dependent progressive brain neurodegeneration and axonal degeneration, significant (P < 0.05) bang sensitivity and convulsions, and cognitive deficits due to up-regulation of the paralytic gene in our mutants. The neuropathological findings were significantly (P < 0.05) alleviated in dose and duration-dependent fashions to near normal/normal after acute and chronic treatment with extract similar to sodium valproate. Therefore, para is expressed in neurons of brain tissues in our mutant flies to bring about epilepsy phenotypes and behaviors of the current juvenile and old-adult mutant D. melanogaster models of epilepsy. The herb exerts neuroprotection by anticonvulsant and antiepileptogenic mechanisms in mutant D. melanogaster due to plant flavonoids, polyphenols, and chromones (1 and 2) which exert antioxidative and receptor or voltage-gated sodium ion channels’ inhibitory properties, and thus causing reduced inflammation and apoptosis, increased tissue repair, and improved cell biology in the brain of mutant flies. The methanol root extract provides anticonvulsant and antiepileptogenic medicinal values which protect epileptic D. melanogaster. Therefore, the herb should be advanced for more experimental and clinical studies to confirm its efficacy in treating epilepsy.

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Prophylactic efficacy of Boerhavia diffusa L. aqueous extract in toluene induced reproductive and developmental toxicity in Drosophila melanogaster

Cited by 14 publications

References 22 publications

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

Toxicity Assessment of Curculigo orchioides Leaf Extract Using Drosophila melanogaster: A Preliminary Study

Towards a reporting guideline for developmental and reproductive toxicology testing in C. elegans and other nematodes

Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models

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