Prophylactic immunization to Helicobacter pylori infection using spore vectored vaccines

Abstract

Helicobacter pylori (HP) is a Gram-negative human pathogenic bacterium believed to be carried by an estimated 50% of the world's population. 1 Infection typically occurs in childhood and if untreated an individual will likely remain colonized for life. 2 Of those infected about 15% may go on to develop pathological symptoms, typically chronic gastritis and peptic ulcer disease (gastric and duodenal ulceration) with the potential for gastric cancer (adenocarcinoma and lymphoma). H. pylori infection is thought to be linked with more than 90% of gastric cancers and normally results if the infection is left untreated. 3 Gastric adenocarcinoma is now the 5th most common cancer and the 3rd in terms of cancer-related deaths. Unfortunately, by the time gastric cancer has developed it is nearly impossible to

Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia

“…[62]. Nevertheless, albeit without statistical significance, this observation is consistent with our previous study [16], which also demonstrated a 40 % reduction.…”

“…These results are consistent with previous studies conducted by Zhou et al . [62] using spores expressing the full-length UreB protein (CotC-UreB) and our recent studies employing spores expressing the full-length UreA protein (CotB-UreA) (strain PK82) or truncated UreB domain (CotB-UreB) (strain PK78) [16]. Both strains A1.13 and PK82 expressing CotB fused with full-length UreA protein, but CotB sequences were designed differently from amino acids 1 to 281 in A1.13 [31] and from amino acids 1 to 338 in PK82 [16].…”

“…To evaluate the expression of UreA in the B. subtilis strains, Western blotting and whole-spore enzyme-linked immunosorbent assay (ELISA) were performed as described in our previous study [16].…”

“…On the 62nd day, the mice were sacrificed and serum samples were collected via cardiac bleed. The analysis of immunological responses of these faecal and serum samples was performed as detailed in our recent study [16].…”

“…In our recent research, we have introduced an H. pylori HP34 strain in Vietnam for preclinical test of vaccine candidates [16] but this clarithromycin-resistant strain has not been analysed in terms of genomics and histopathology to identify the genotype and pathological mechanism, which can be coupled with and compared to wider geographical and disease infection contexts. This study aims to address the existing knowledge gap concerning H.…”