Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia‐Reperfusion in the Rat

Önal, Özkan; Yetışır, Fahri; Sarer, A. Ebru Salman; Zeybek, Naciye Dilara; Önal, Ceren; Yürekli, Banu Şarer; Çelik, Hüseyin; Sirma, Ayse; Kılıç, Mehmet

doi:10.1155/2015/792016

Cited by 31 publications

(30 citation statements)

References 27 publications

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“…Ozone regulates cellular antioxidant systems, such as SOD, glutathione, and enzymatic reactions, preparing the host to confront the pathophysiologic conditions regulated by oxidative stress (Kesik et al, 2009). In this study, we demonstrated that ozone significantly increased some key antioxidant parameters, such as SOD, GSH‐Px, and CAT, which was consistent with the previous reports (Rodriguez et al, 2009; Onal et al, 2015). The cell injury caused by oxidative stress could increase MDA, and significant decrease of MDA after ozone treatment indicated that the mitochondrial oxidative damage induced by I/R injury was markedly reduced by ozone.…”

Section: Discussionsupporting

confidence: 93%

“…It can oxidize many microorganisms and its related toxins at different rates, and prepare the host to face physiopathological events regulated by reactive oxygen species (ROS) (Onal et al, 2015). Ozone has been proved to protect several organs in the animal models of I/R injury (Chen et al, 2008; Onal et al, 2015). However, it is not clear how ozone prevents or attenuates cerebral I/R injury.…”

Section: Discussionmentioning

confidence: 99%

“…Ozone, a reactive gas, has been proved to play an important role in inflammation as an immune effector, and has been used to treat many diseases (Meng et al, 2017). Pretreatment with small doses of ozone, has recently been proved to protect against I/R injury in animal models of cerebra, hepatic, renal, and cardiac ischemia (Chen et al, 2008; Onal et al, 2015). Furthermore, ozone may improve cerebral I/R injury by regulating inflammatory response and increasing blood flow (Clavo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

Cai

Zheng

et al. 2020

Cell Biology International

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Cerebral ischemia/reperfusion (I/R) injuries are common and often cause severe complications. Ozone has been applied for protecting I/R injury in animal models of several organs including cerebra, but the detailed mechanism remains unclear. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase measurement were used to determine the influence of ozone on cell activity and damage of SH‐SY5Y cells. Some redox items such as catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH‐Px), and superoxide dismutase (SOD) were measured by enzyme‐linked immunosorbent assay. The mitochondrial membrane potential (ΔΨm) was determined by JC‐1 assay. Cytochrome‐c (cyt‐c) level in the cytoplasm and mitochondrion was measured by western blotting. Apoptosis was determined by flow cytometry, and some apoptosis‐related molecules were detected by quantitative real‐time polymerase chain reaction and western blotting. Ozone alleviated oxidative damage by increasing GSH‐Px, SOD, CAT, and decreasing MDA. Ozone decreased mitochondrial damage caused by I/R injury and inhibited the release of cyt‐c from mitochondrion to cytoplasm in SH‐SY5Y cells. The cell apoptosis caused by I/R was inhibited by ozone, and ozone could decrease apoptosis by increasing the ratio of Bcl‐2/Bax and inhibiting caspase signaling pathway in SH‐SY5Y cells. Ozone has the ability of maintaining redox homeostasis, decreasing mitochondrion damage, and inhibiting neurocytes apoptosis induced by I/R. Therefore, ozone may be a promising protective strategy against cerebral I/R injury.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

Cai

Zheng

et al. 2020

Cell Biology International

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“…During the inflammatory response, there are changes in the ratio of leukocytes in circulation. Hypoxia occurring in situations of spinal cord ischemia may be seen biochemically due to acidosis, free radical damage and membrane disruption [15]. Medical ozone is a chemical agent that consists of three oxygen atoms and has antioxidant, angiogenic and vasodilator effects.…”

Section: Discussionmentioning

confidence: 99%

The ameliorative effect of ozone therapy on spinal cord ischemia in rabbits

Akman¹

2020

Ann Clin Anal Med

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Aim: Research in applications of O3 treatment for spinal cord ischemia is a rich area of study. The effects of O3 treatment on spinal cord ischemia have not been studied much to date. The aim of this study is to investigate the role of prophylactic administration of O3 on the recovery of ischemic changes in terms of oxidative stress markers in lysosomal and mitochondrial enzymatic activities in the spinal cord of male rabbits. Material and Method: Twenty-four New Zealand White (NZW) adult male rabbits (weight: 2.5-3 kg) were obtained from Saki Yenilli Experimental Animals Center (Ankara, Turkey). The rabbits were housed in plastic cages at a temperature of 21 ± 2 °C, with 12 hours light/12 hours dark cyclus, fed with standard rabbit diet (Bil-Yem Ltd. Co., Ankara, Turkey) and water ad libitum for the duration of the experiment. Rabbits were randomly divided into 4 groups of 6 rabbits. The first group was accepted as control and the second group was treated with 50 μg/mL (20 mL) ozone by rectal insufflation 5 times per day. On the 10th day, animals were sacrificed. The third sham group had infrarenal abdominal aorta cross-clamping (IAACC) performed and were sacrificed 24 hours later. The fourth group was treated with 50 μg/mL ozone (20 mL) by rectal insufflation 5 times per day and on the 10th day, IAACC was performed. Twenty-four hours later the animals were sacrficed. Results: When data obtained from the biochemical analysis were investigated (Table 1) in the groups with induced ischemia, the expected reduction in SOD, CAT, and GPX enzyme activities was observed and an increase in MPO activity was observed. The statistical difference between ozone administered groups and the control group was significant (p < 0.05). When the ischemia+ozone group is compared with the ischemia group, the results are significant and a statistical difference is observed (p < 0.05). When GPX values in the group with ischemia-induced were investigated, the enzyme activity values were very low. A serious increase was observed in the ozone administered treatment group (p < 0.05). Discussion: Ozone (O3) is a molecule carrying three oxygen atoms forming as a result of exposure of oxygen to high energy electric currents and ultraviolet (UV) light in nature. It is found as a gas at room temperature. It is colorless, and has a characteristic smell. As ozone has a high degree of oxidation strength, it is called "active oxygen" in medicine. Due to its ability to increase blood circulation, it is used for treatment, not just of disorders related to circulation, but as an aid to the renewal of organic functions disrupted by many diseases.

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“…Sections were examined and scored by pathologists blinded to each other's observations. Pathological damage to the intestinal tissues in each group was analyzed semiquantitatively: 0 indicated normal mucosal villi; 1 indicated the subepithelial space above the villi; 2 indicated moderate elevation of the superior cortex of the lamina propria; 3 indicated large epithelial uplift on both sides of the villi (a few tips may fall off); 4 indicated villus shedding, lamina propria exposure, and telangiectasia; and 5 indicated lamina propria calving, hemorrhage, and ulcer [20][21][22].…”

Section: Histologymentioning

confidence: 99%

ADAR1 Alleviates Inflammation in a Murine Sepsis Model via the ADAR1-miR-30a-SOCS3 Axis

Zhou

Liu

Zhao

et al. 2020

Mediators of Inflammation

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Adenosine deaminase acting on double-stranded RNA 1 (ADAR1) mediates adenosine-to-inosine (A-to-I) RNA editing events. ADAR1 is highly expressed in “septic” macrophages and in small intestinal tissues of mice with sepsis. Overexpression of ADAR1 suppresses inflammation and intestinal damage. However, the specific underlying mechanism is unclear. This study was conducted to explore how microRNA (miRNA) regulates the anti-inflammatory mechanism of macrophages following ADAR1 upregulation. A murine sepsis model was established by cecal ligation and puncture (CLP). Mice were randomly assigned to sham, CLP, and CLP+ADAR1 groups. Hematoxylin and eosin (HE) staining and fluorescence isothiocyanate-dextran were used to evaluate intestinal injury and permeability. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting, and Luminex assays were performed to detect changes in the expression of inflammatory cytokines. Adenoviruses were used to express ADAR1 in RAW 264.7 cells. Ribonucleoprotein immunoprecipitation analysis was conducted to detect the binding of ADAR1 and miRNAs. A dual-luciferase reporter assay was used to detect the binding of miRNAs and regulatory factors. We observed that ADAR1 significantly increased the expression of suppressor of cytokine signaling 3 (SOCS3) in macrophages and reduced the expression of interleukin-6 in macrophages and the serum, thereby reducing intestinal permeability and mucosal injury in mice with sepsis. The RNA-ribonucleoprotein immunoprecipitation binding assay and qRT-PCR demonstrated a direct interaction between ADAR1 and pri-miR-30a. The luciferase assay demonstrated that SOCS3 was significantly inhibited by miR-30a-5p, the mature product of miR-30a. Thus, ADAR1 exerts a protective effect against sepsis by reducing inflammation and organ damage via the ADAR1-miR-30a-SOCS3 axis.

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Prophylactic Ozone Administration Reduces Intestinal Mucosa Injury Induced by Intestinal Ischemia‐Reperfusion in the Rat

Cited by 31 publications

References 27 publications

Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

The ameliorative effect of ozone therapy on spinal cord ischemia in rabbits

ADAR1 Alleviates Inflammation in a Murine Sepsis Model via the ADAR1-miR-30a-SOCS3 Axis

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