2019
DOI: 10.1007/s40201-019-00359-w
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Propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR) assay for rapid detection of viable and viable but non-culturable (VBNC) Pseudomonas aeruginosa in swimming pools

Abstract: Lack of culturability in the viable but non-culturable (VBNC) bacteria and the ability to regain infectivity in favourable conditions is one of the new challenges of public health providers for Pseudomonas aeruginosa monitoring in environmental samples. Propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) is one of the promising methods for timely detection of VBNC pathogens in environmental samples. We developed and used a method for the first time to detection of VBNC P. aeruginosa in swimmi… Show more

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Cited by 29 publications
(15 citation statements)
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“…The PMA-qPCR technology has been applied to the detection of plant pathogen cells [ 26 , 29 , 30 ]. Despite the fact that F .…”
Section: Discussionmentioning
confidence: 99%
“…The PMA-qPCR technology has been applied to the detection of plant pathogen cells [ 26 , 29 , 30 ]. Despite the fact that F .…”
Section: Discussionmentioning
confidence: 99%
“…The use of PMAxx combined with qPCR is therefore one possible solution for Leptospira measurement; it is time saving and avoids overestimation. By detecting non-permeable and VBNC bacteria, this method pinpointed and prevented a Leptospira outbreak originating from environmental water [ 58 ]. These data could be useful for quantitative microbiological risk assessment (QMRA) approaches.…”
Section: Discussionmentioning
confidence: 99%
“…One promising approach to resolving this overestimation issue is propidium monoazide qPCRs (PMA-qPCRs). PMA is a DNA intercalating molecule that penetrates only membrane-damaged or membrane-compromised cells (i.e., dead cells), binds to DNA within the dead cells, and inhibits DNA amplification during subsequent qPCRs ( Nocker et al, 2007 ; Li and Chen, 2013 ; Golpayegani et al, 2019 ; Guo et al, 2021 ). The DNA or genetic markers of Legionella within membrane-intact (i.e., live, including culturable and VBNC) cells are not bound to PMA and are normally amplified during qPCRs.…”
Section: Key Features Of Legionella That Make It A...mentioning
confidence: 99%