Propionibacterium australiense sp. nov. Derived from Granulomatous Bovine Lesions

Bernard, Kathryn; Shuttleworth, Lucas A.; Munro, C.; Forbes-Faulkner, J.C.; Pitt, D.; Norton, J. H.; Thomas, A.D.

doi:10.1006/anae.2000.0408

Cited by 59 publications

(32 citation statements)

References 6 publications

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“…The two NML strains were also tested using the API Coryne kit in parallel to conventional methods that included casein, tyrosine, and starch hydrolysis (2). Products of fermentation for 91-0031 and 92-0360 were analyzed as described previously (3).…”

Section: Methodsmentioning

confidence: 99%

Corynebacterium nigricans sp. nov.: Proposed Name for a Black-Pigmented Corynebacterium Species Recovered from the Human Female Urogenital Tract

et al. 2003

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Six independent isolates of an unusual black-pigmented Corynebacterium species (strains CN-1, CN-2, CN-3415, W70124, 91-0032, and 92-0360) were recovered from the human female urogenital tract. Four of the six source patients had complications of pregnancy, including spontaneous abortion, preterm labor, and low amniotic fluid volume at the time of the pathogen isolation. One isolate was recovered from a vaginal ulcer. All six strains yielded black-pigmented colonies on sheep blood agar, chocolate agar, and colistin-nalidixic acid agar after 24 to 48 h of incubation at 35°C. The dry, adherent colonies pitted the agar surface. The cells were coccobacillary to rod-shaped, catalase positive, nonmotile, and nonlipophilic. Only five of six isolates were available for characterization. Biochemical and chemotaxonomic studies revealed that the strains belong to the genus Corynebacterium but differ from known corynebacterial species. Comparative 16S rRNA gene sequence analysis showed that the strains are closely related and form a new subline within the genus Corynebacterium. We propose the name Corynebacterium nigricans sp. nov. for this group of coryneforms. The type strain of Corynebacterium nigricans is CN-1. It is deposited in the American Type Culture Collection (assigned strain number ATCC 700975) and in the Institute Pasteur collection (assigned strain number CIP 107346).The female urogenital tract is a natural habitat for a multitude of commensal microbes, including diphtheroids. In many instances, these microbes and their metabolites serve as barriers against colonization by harmful pathogens that may cause different diseases. The reported commensal organisms of the female urogenital tract are species of Lactobacillus, Staphylococcus, Streptococcus, Neisseria, Mycoplasma, Ureaplasma, enteric organisms, Acinetobacter, Capnocytophaga, Enterococcus, Bacteroides, Porphyomonas, Prevotella, Fusobacterium, Mobiluncus, Peptostreptococcus, Propionibacterium, Actinomyces, Bifidobacterium, Candida, and coryneform bacteria (8). Like other resident microbes of the human body, some of the vaginal microbiota also are opportunistic pathogens, which become pathogenic when the host immune status is compromised, suppressed, or under immunologic stress. A number of bacterial species are associated with urogenital-related diseases, and among the Corynebacterium species, C. lipophiloflavum, C. amycolatum, C. glucuronolyticum, C. riegelii, and C. urealyticum have been implicated (4, 6-8). The CDC coryneforms group 4 and C. lipophiloflavum are reported to cause urinary tract infections and bacterial vaginosis, respectively (5, 9).Through the use of nucleic acid-based detection and characterization methods, such as 16S rRNA gene sequence analysis, many new pathogenic organisms are continually being identified, characterized, and linked to diseases of known and unknown etiology. This approach has been successfully applied in our laboratory to identify and characterize a number of fastidious microorganisms (12,(15)(16)(17)). An unusual ...

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Section: Methodsmentioning

confidence: 99%

Corynebacterium nigricans sp. nov.: Proposed Name for a Black-Pigmented Corynebacterium Species Recovered from the Human Female Urogenital Tract

et al. 2003

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“…australiense came from granulomatous bovine lesions [12] and P. acidifaciens from human carious dentine [13]. Recently, a new species isolated from human humerus, P. humerusii, has been proposed [14].…”

Section: Introductionmentioning

confidence: 99%

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

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“…The organism did not grow in PY (peptone-yeast extract) broth alone or augmented with dextrose, serum, Tween 80, bile, or formate-fumarate. No growth or negative results were obtained for a panel of more than 30 PY sugars and substrates used for identification of anaerobes (1). No growth was obtained with an API 32A panel (bioMérieux).…”

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confidence: 98%

“…No growth was obtained with an API 32A panel (bioMérieux). Cellular fatty acid (CFA) analysis was performed in duplicate, as described previously, except that growth from 5 BBA plates was harvested after 5 days of growth anaerobically at 35°C, combined into 1 broth containing PY with dextrose plus Tween, incubated for 48 h, and then extracted (1). For each attempt, the organic phase required further concentration to bolster counts.…”

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confidence: 99%

Novel Fastidious, Partially Acid-Fast, Anaerobic Gram-Positive Bacillus Associated with Abscess Formation and Recovered from Multiple Medical Centers

et al. 2013

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We report a novel anaerobe causing abscess in four patients at three hospitals. In the clinical specimen, bacilli were branching, Gram positive, and acid fast. The organism grew slowly and was not identified by 16S rRNA sequencing. Our findings support the description of a new genus and species of the suborder Corynebacterineae. CASE REPORTSC ase 1. A 65-year-old male with metastatic prostate cancer diagnosed in Cleveland, OH, in March 2009 presented 1 June 2011 with an enlarging mass adjacent to a surgical incision at T3-8 from previous spinal stabilization at the time of diagnosis. Medications included prednisone (5 mg daily), docetaxel, zoledronic acid, and leuprolide. There was no significant travel history, exposure to animals, or sick contacts. He denied trauma to the upper back and had a sedentary life. A physical exam revealed a weight of 130 kg, blood pressure of 106/78, pulse of 160/min (atrial fibrillation), temperature of 38.6°C, and respiratory rate of 22/min. He was unaware of his fever. The mass was minimally warm to the touch, mildly erythematous, fluctuant, nondraining, and nonpainful. The white blood cell (WBC) count was 12,370/l with 77% neutrophils.The magnetic resonance imaging (MRI) and computed tomography (CT) scan were consistent with thoracic fluid collection and abscess. Surgical incision and drainage produced a large amount of purulent, nonodorous fluid. Pedicle screws placed previously were solidly in position, and there was no evidence of bony destruction. Partial hardware removal and debridement of necrotic tissue were performed. A swab of the subfascial tissue, fluid, and hardware were submitted for culture. A beaded Gram-positive, branching bacillus and inflammatory cells were present on Gram stains of all material submitted. Modified Kinyoun stain was positive. (Fig. 1a and b).Vancomycin started prior to surgery was switched to ampicillin-sulbactam but changed to meropenem due to suspicion of Nocardia. Blood cultures produced no growth. The white blood cell count normalized, and he was afebrile by postoperative day 2. His surgical site healed well, and he was discharged postoperative day 7 on meropenem. After 4 weeks, home antibiotic therapy was switched to sulfamethoxazole-trimethoprim (SXT) at 800 to 160 mg with two tablets 3 times per day (TID). Docetaxel chemotherapy was resumed.Anaerobic cultures of the fluid and hardware grew after 13 days of incubation. Cultures performed from swabs of tissue did not grow, nor was growth obtained from any specimen submitted for culture for mycobacteria, fungi, and aerobic bacteria. By partial 16S rRNA sequencing, the organism was Ͻ95% identical to Dietzia, Tsukamurella, and Corynebacterium spp. The 16S rRNA sequence (1,445 bp) was later submitted to GenBank. With these results, therapy was changed to amoxicillin-clavulanate (1,000/ 62.5 mg twice daily).Five weeks postoperatively, a scant amount of clear yellow nonodorous fluid was noted draining from a pinhole-sized opening of a 3-cm fluctuant, nonerythematous collection at the surgical si...

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Propionibacterium australiense sp. nov. Derived from Granulomatous Bovine Lesions

Cited by 59 publications

References 6 publications

Corynebacterium nigricans sp. nov.: Proposed Name for a Black-Pigmented Corynebacterium Species Recovered from the Human Female Urogenital Tract

Corynebacterium nigricans sp. nov.: Proposed Name for a Black-Pigmented Corynebacterium Species Recovered from the Human Female Urogenital Tract

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Novel Fastidious, Partially Acid-Fast, Anaerobic Gram-Positive Bacillus Associated with Abscess Formation and Recovered from Multiple Medical Centers

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