Propionibacterium freudenreichii CIRM-BIA 129 Osmoadaptation Coupled to Acid-Adaptation Increases Its Viability During Freeze-Drying

Kponouglo, Koffigan; Rabah, Houem; Bonnassié, Sylvie; Ossemond, Jordane; Pottier, Sandrine; Jardin, Julien; Briard‐Bion, Valérie; Marchand, Pierre; Blanc, Philippe; Jeantet, Romain; Jan, Gwénaël

doi:10.3389/fmicb.2019.02324

Cited by 17 publications

(10 citation statements)

References 87 publications

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“…Acid tolerance catabolic activities were also variable between species. All species contained L-asparaginase, which has been associated with acid tolerance in P. freudenreichii [ 94 ]. The arginine deiminase pathway and associated antiporter were identified in P. avidum and P. acnes , while the glutamate decarboxylase and associated antiporter were found in P. freudenreichii .…”

Section: Resultsmentioning

confidence: 99%

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

McCubbin

Gonzalez-Garcia

Palfreyman

et al. 2020

Genes

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Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. This combined approach gave us new insights into the evolution of Propionibacterium metabolism and led us to propose a novel, putative ferredoxin-linked energy conservation strategy. The pan-genomic approach highlighted key differences in Propionibacterium metabolism that reflect adaptation to their environment. Results were mathematically captured in genome-scale metabolic reconstructions that can be used to further explore metabolism using metabolic modeling techniques. Overall, the data provide a platform to explore Propionibacterium metabolism and a tool for the rational design of strains.

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Section: Resultsmentioning

confidence: 99%

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

McCubbin

Gonzalez-Garcia

Palfreyman

et al. 2020

Genes

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“…A wide range of genetic and environmental optimizations have been conducted to improve these properties [6,29]. Moreover, some optimizations of the growth and processing conditions allowed the improvement of resistance towards storage and towards several industrial processes, such as freeze-drying and spray-drying [30][31][32][33].…”

Section: Technological Importancementioning

confidence: 99%

Propionibacterium freudenreichii: General Characteristics and Probiotic Traits

Rodovalho¹,

Rodrigues²,

Jan³

et al. 2022

Prebiotics and Probiotics - From Food to Health

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Propionibacterium freudenreichii is a Gram-positive dairy probiotic bacterial species that has been used as a ripening starter in the production of Swiss-type cheese for a long time. It has been exploited for the optimization of cheese production, including ripening capacities and aroma compounds production, but also for the production of vitamin B12 and organic acids. Furthermore, it has emerged in the probiotics landscape owing to several beneficial traits, including tolerance to stress in the gastrointestinal tract, adhesion to host cells, anti-pathogenic activity, anticancer potential and immunomodulatory properties. These beneficial properties have been confirmed with in vitro and in vivo investigations, using several omics approaches that allowed the identification of important molecular actors, such as surface proteins, short-chain fatty acids and bifidogenic factors. The diversity within the species was shown to be an important aspect to take into consideration, since many of these properties were strain-dependent. New studies should dive further into the molecular mechanisms related to the beneficial properties of this species and of its products, while considering the complexities of strain diversity and the interactions with the host and its microbiota. This chapter reviews current knowledge on the possible impact of P. freudenreichii on human health.

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“…Such combinations in turn triggered intracellular accumulation of stress proteins, of compatible solute glycine betaine, as well as modulations of membrane fatty acids composition. These rearrangements led to enhanced survival of P. freudenreichii upon freeze-drying (Gaucher et al 2019c). Spray drying constitutes a more productive and efficient way to produce dried cultures, but a more stressing one, imposing severe oxidative and thermal challenges.…”

Section: Adaptation Of P Freudenreichii Ameliorates Its Stress Tolerance and Drying Survivalmentioning

confidence: 99%

“…Many studies thus aimed at improving this process by limiting bacterial death during spray-drying (Broeckx et al 2016). In our experience, dairy propionibacteria including P. freudenreichii are freeze-dried without major cell damage, so more than 50% survival is classically observed, whatever the growth medium used (Gaucher et al 2019c). However, the first attempts to spray-dry P. freudenreichii without specific adaptation after growth in YEL medium led to less than 4% survival (Huang et al 2016b).…”

Section: New Drying Technologies Are Developedmentioning

confidence: 99%

Improving the drying of Propionibacterium freudenreichii starter cultures

Jeantet

Jan

2021

Appl Microbiol Biotechnol

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Propionibacterium freudenreichii is a beneficial food-grade actinobacterium, widely implemented, and thus consumed, in various food products. As the main application, P. freudenreichii is used as a cheese-ripening starter, mostly in hard type cheeses. Indeed, during manufacture of "Swiss-type" cheeses (or opened-body cheeses), the technological process favors propionibacteria growth, as well as the corresponding propionic fermentation. This leads to the characteristic flavor of these cheeses, through the release of short chain fatty acids and through lipolysis, as well as to their specific texture. To fulfil this ripening, massive amounts of propionibacteria are industrially produced, dried and stored, prior to cheese making. Furthermore, P. freudenreichii is commercialized in various probiotic food supplements aiming at preserving intestinal health and comfort, in line with its ability to produce beneficial metabolites (short chain fatty acids, vitamins), as well as immunomodulatory compounds. Other industrial applications of P. freudenreichii include the production of food-grade vitamins of the B group, of trehalose, of conjugated linoleic acid, and of biopreservatives. For these different applications, maintaining survival and activity of propionibacteria during production, drying, storage and finally implementation, is crucial. More widely, maintaining live and active probiotic bacteria represents a challenge as the market for probiotic products increases. Probiotic bacteria are, for a bulk majority, freeze-dried, but spray drying is also more and more considered. Indeed, this process is both continuous and more cost-efficient, as it utilizes less energy compared to freeze-drying; on the other hand, it exposes bacteria to higher heat and oxidative stresses. Apart from process optimization and strain selection, it is possible to enhance the resistance of bacteria by taking advantage of their adaptation capacity. Indeed, P. freudenreichii stress tolerance can be boosted by different pretreatments applied before the drying step, thus considerably increasing its final survival. In particular, adaptation to hyperosmotic conditions improves stress tolerance, while the presence of osmoprotectants may mitigate this improvement. Thermal adaptation also modulates tolerance towards these technological challenges. The composition of the growth medium, including the ratio between the carbohydrates provided and the non-protein nitrogen, plays a key role in driving the accumulation of osmoprotectants. This, in turn, determines P. freudenreichii tolerance towards different stresses, and overall towards both freeze-drying and spray-drying. As an example, the accumulation of trehalose enhances its spray-drying survival, while the accumulation of glycine betaine enhances its freeze-drying survival. Growth of propionibacteria in hyperconcentrated whey was used to trigger multiple stress tolerance acquisition, underpinned by overexpression of key stress protein, accumulation of cytoplasmic storage compounds, and leading to enhanc...

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Propionibacterium freudenreichii CIRM-BIA 129 Osmoadaptation Coupled to Acid-Adaptation Increases Its Viability During Freeze-Drying

Cited by 17 publications

References 87 publications

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity

Propionibacterium freudenreichii: General Characteristics and Probiotic Traits

Improving the drying of Propionibacterium freudenreichii starter cultures

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