Propofol Inhibits Lung Cancer A549 Cell Growth and Epithelial‐Mesenchymal Transition Process by Upregulation of MicroRNA-1284

Abstract

Propofol has been widely used in lung cancer resections. Some studies have demonstrated that the effects of propofol might be mediated by microRNAs (miRNAs). This study aimed to investigate the effects and mechanisms of propofol on lung cancer cells by regulation of miR-1284. A549 cells were treated with different concentrations of propofol, while transfected with miR-1284 inhibitor, si-FOXM1 and their negative controls. Cell viability, migration, invasion, the expression of miR-1284, FOXM1 and epithelial mesenchymal transition (EMT) factors were respectively detected by CCK8, Transwell, qRT-PCR and western blot assays. In addition, the regulatory and binding relationships among propofol, miR-1284 and FOXM1 were assessed, respectively. Results showed that propofol suppressed A549 cells viability, migration and invasion, up-regulated E-cadherin, while down-regulated N-cadherin, Vimentin and Snail expressions. Moreover, propofol significantly promoted the expression of miR-1284. MiR-1284 suppression abolished propofol-induced decreases of cell viability, migration and invasion, and increased FOXM1 expression and the luciferase activity of FOXM1-wt. Further, miR-1284 negatively regulated FOXM1 expression. FOXM1 knockdown reduced cell viability, migration and invasion by propofol treatment plus miR-1284 suppression. In conclusion, our study indicated that propofol could inhibit cell viability, migration, invasion and EMT process in lung cancer cells by regulation of miR-1284.

Propofol inhibits pancreatic cancer proliferation and metastasis by up‐regulating miR‐328 and down‐regulating ADAM8

“…In fact, several previous studies have implicated the functions of propofol on miRNA expression. For example, miR‐372, miR‐195, miR‐1284, miR‐206, miR‐9 and miR‐21 were all stated to be affected by propofol to control cell growth, migration and invasion . Additionally, here ADAM8 was identified to directly target to miR‐328.…”

“…For example, miR-372, miR-195, miR-1284, miR-206, miR-9 and miR-21 were all stated to be affected by propofol to control cell growth, migration and invasion. [14][15][16][17][18][19] Additionally, here ADAM8 was identified to directly target to miR-328. Our results implicate that propofol exerts an inhibitory effect on pancreatic cancer cell growth, migration and invasion by enhancing miR-328 which targets ADAM8.…”

The mechanism of propofol in cancer development: An updated review

“…It is well known that epigenetic alterations or genetic modulation of epigenetic regulators plays important roles in carcinogenesis . Many studies show that the anticancer effect of propofol is potentially due to regulation of the epigenetic pathway in cancer …”

“…Liu et al. proved that propofol inhibited lung cancer cell growth and EMT through upregulation of miR‐1284 . miR‐218 acted as a tumor suppressor in many cancers, and propofol could upregulate miR‐218 expression.…”

“…6 Liu et al proved that propofol inhibited lung cancer cell growth and EMT through upregulation of miR-1284. 7 miR-218 acted as a tumor suppressor in many cancers, 8,9 and propofol could upregulate miR-218 expression. Xu et al proved that propofol inhibited glioma cell proliferation and invasion by upregulating miR-218 expression.…”