Propolis alleviated Cisplatin-induced hepatorenal oxidative stress and apoptosis

Abstract

The present research was planned to evaluate the protective effect of propolis water extract against cisplatin hepato-renal toxicity. Twenty-four male albino rats were divided into four equal groups. G1 (control) administered water only; G2 (propolis) administered (100 mg extract of propolis/kg body weight) orally by stomach tube at a once-daily dose for 14 days; G3 (cisplatin) administered once intraperitoneally (7.5 mg cisplatin/kg body weight) at day 10 of the experiment; and G4 (cisplatin+propolis) administered like G2 and G3. The serum biochemical analysis in the cisplatin group revealed an increase in AST, ALT, ALP, cholesterol, triglyceride, LDL-cholesterol, urea, and creatinine concentrations and a decrease in total protein and albumin concentrations. Moreover, cisplatin-treated rats significantly increased MDA levels and decreased SOD and CAT levels in hepatic and renal tissue compared to the control group. Administration of propolis (100 mg extract of propolis/kg.b.wt.) orally by stomach tube one hour before the second drug, which is once intraperitoneal (7.5 mg cisplatin/kg.b.wt.), at day 10 of the experiment (day number ten of the experiment, which continued for 14 days), reversed induced hepatorenal damage of the cisplatin group by exerting antioxidant and antiinflammatory effects. Histopathological and immuno-histochemical examinations showed the hepatorenal damage induced by cisplatin was attenuated by propolis. In conclusion, propolis may help to lessen the hepatorenal damage induced by cisplatin via its antioxidant and antiapoptotic activities.